Antioxidant, Anti-Inflammatory, and Analgesic Properties of Chemically Characterized Polyphenol-Rich Extract from Withania adpressa Coss. ex Batt

Abstract

The current work was undertaken to investigate the chemical composition, antioxidant, anti-inflammatory, and analgesic properties of a polyphenol-rich fraction from Withania adpressa Coss. ex Batt. After being extracted, the polyphenol-rich fraction was chemically characterized through use of high-performance liquid chromatography (HPLC). Antioxidant potency was assessed through the use of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant capacity (TAC). Inflammatory and analgesic properties were assessed in vivo through the use of carrageenan and heat stimulus assays, respectively. Chromatographic analysis of polyphenol-rich fraction revealed the presence of potentially bioactive phenols including epicatechin, apigenin, luteolin, quercetin, caffeic acid, p-coumaric acid, and rosmarinic acid. The polyphenol-rich fraction showed interesting anti-free-radical potency with a calculated IC50 value of 27.84 ± 1.48 µg/mL. At the highest dose used (1000 µg/mL), the polyphenol-rich fraction scored good total antioxidant capacity with a calculated value of 924.0 ± 28.29 µg EAA/mg. The polyphenol-rich fraction strongly alleviated the inflammatory effect of carrageenan injected into the plantar fascia of rats resulting in inhibition up to 89.0 ± 2.08% at the highest tested dose (500 mg/kg). The polyphenol-rich fraction showed a good analgesic effect wherein the delay in reaction time to a thermal stimulus caused by 500 mg/kg had a highly similar effect to that induced by Tramadol used as a positive control. The findings of the current work highlight the importance of polyphenol-rich fractions from W. adpressa Coss. ex Batt. as an alternative source of natural antioxidant, inflammatory, and analgesic drugs to control relative diseases.

The Phenolic Content of Pistacia lentiscus Leaf Extract and Its Antioxidant and Antidiabetic Properties

“…On the other hand, the diferent organs of the plant under study were tested as potential natural antioxidants, where the highest activity was observed in leaf extracts with IC 50 � 0.166 ± 0.001 mg/mL compared to the stems, fruits, and roots with the following values, 0.076 ± 0.001 mg/mL, 0.772 ± 0.043 mg/ml, and 0.370 ± 0.002 mg/mL, respectively [45]. Based on the literature, Ouahabi et al reported that the Pistacia lentiscus leaf extract possesses β-carotene bleaching activity comparable to the value obtained in our study, with an IC 50 value of 0.19 mg/ml [42]. Our obtained results have shown that the high polyphenol content could probably afect the oxidative power of the extract, which is in accordance with the literature search which indicated that higher antioxidant activity was related directly to the high amount of phenolic compounds [46].…”

“…Also, our fndings are consistent with those reported by Ouahabi et al; this applies to the leaves of Pistacia lentiscus collected in the eastern region of Morocco. It was found that the extract contains many phenolic compounds, such as catechin, quercetin, and p-coumaric acid, along with other notable compounds [42].…”

Mitigation of oxidative stress and inflammatory factors, along with the antibrowning and antimicrobial effects of cassia seed microbial fermentation solution

“…However, the medium and high-dosage groups display higher T-AOC levels than the model group ( p > 0.05). The antioxidant prowess of cassia seed fermentation solution can be attributed to its antioxidant constituents, such as polyphenols, known to inhibit free radical activity, thereby mitigating tissue damage and aging ( Salamatullah, 2022 ). This notably elevates the CAT content within zebrafish ( p < 0.01).…”

Plant-Derived Natural Products and Their Biomedical Properties: Recent Advances and Future Directions

“…Salamatullah investigated the chemical composition, as well as the antioxidant, anti-inflammatory, and analgesic properties, of a polyphenol-rich fraction from Withania adpressa Coss. ex Batt [9]. High-performance liquid chromatography (HPLC) was applied and revealed bioactive phenols including epicatechin, apigenin, luteolin, quercetin, caffeic acid, p-coumaric acid, and rosmarinic acid.…”