Protective effect of Cassia fistula fruit extract against bromobenzene-induced liver injury in mice

Abstract

<jats:p> In the present study, hepatoprotective effect of Cassia fistula fruit extract was investigated in mice. Animals were divided into six groups receiving normal saline (1), bromobenzene (460 mg/kg) alone (2) and together with increasing doses (200, 400, 600, 800 mg/kg) of a crude hydro-alcoholic extract of Cassia fistula fruit (3-6, respectively). All administrations were carried out orally, daily, for 10 days. On the 11th day, animals were sacrificed. Serum activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (γGT) were determined; serum levels of direct and total bilirubin were measured; furthermore, livers were prepared for histological examination. Our results showed that bromobenzene treatment alone elicited a significant increase in activities of AST, ALT, ALP (but not γGT), and it significantly elevated the levels of direct and total bilirubin. Co-treatment with Cassia fistula fruit extract, however, significantly and dose-dependently decreased the above-mentioned enzyme activities (with exception of γGT) and bilirubin levels, producing a recovery to the naive state. The protective effect of Cassia fistula fruit extract against liver injury evoked by bromobenzene was confirmed by histological examination as well. In conclusion, the Cassia fistula fruit extract has significant hepatoprotective effect in our murine model. </jats:p>

Protective Effects of the Hydroalcoholic Extract of Capparis spinosa L. Against Cyclophosphamide-Induced Nephrotoxicity in Mice

“…The solvent was filtered through a Whatman paper and then removed under vacuum in a rotary evaporator until dry. The percentage yield was 16% for a dried hydroalcoholic extract (w/w) (22,23).…”

Ameliorative effects of hydroalcoholic extract of Lavandula officinalis L. on cyclophosphamide-induced nephrotoxicity in mice

“…Material was shade-dried, powdered and also drenched in a 70% aqueous-ethanol solution in a big container for three days with occasional properly shaking. The solvent was filtered through a whatman paper and then was removed under vacuum in a rotary evaporator until dryness (27). The percentage yield was 16% for dried hydroalcoholic extract (w/w).…”

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“…Both extracts exhibited significant antioxidant activity in DPPH, Nitric oxide and Hydroxyl radical induced invitro assay methods. Both extracts showed Dose-Dependent protective effect against lipid peroxidation and free radical generation in liver and kidney homogenates 24,25 . Antioxidant activity of Cassia fistula Linn) flowers in alloxan induced diabetic rats 26 .…”