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doi:10.13040/ijpsr.0975-8232.5(6).2125-30

Cited by 2 publications

(4 citation statements)

References 13 publications

(13 reference statements)

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“…In this study, we have investigated the AQS potential of the renowned medicinal herb Cassia fistula [60] and explored its anti-quorum sensing activity against P. aeruginosa . QS inhibition has emerged as a promising target in a wide variety of bacterial infections following the emergence of antibiotic-resistant phenotypes, which has resulted in a search for new therapeutic alternatives [61].…”

Section: Discussionmentioning

confidence: 99%

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Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Peerzada

Shah

Kharkar

et al. 2023

Journal of Medical Microbiology

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Introduction. Resistance to antibiotics is leading to challenges in the treatment of microbial diseases. One amongst the various approaches to control these pathogens is quorum sensing (QS), which is used to rectify resistance issues. Blocking the bacterial QS circuit is the most reliable anti-virulence therapy to control pathogenicity-associated genes. Pseudomonas aeruginosa is a contagious bacterium that proliferates in the host by using signalling molecules like acyl-homoserine lactones; these molecules generate and disseminate toxins and virulence factors for increasing host infection. Hypothesis. The herb Cassia fistula is known to have antimicrobial, antidiabetic, anti-inflammatory, antitumor medicinal properties amongst others. We hypothesize that its crude extracts will inhibit the QS circuit of Pseudomonas aeruginosa (P. aeruginosa). Aim. The research work was aimed at evaluating anti-quorum sensing and anti-biofilm activity of various crude extracts from Cassia fistula against P. aeruginosa . Methodology. Various extraction methods and solvents were availed for maximum separation, and the extracts were screened for anti-quorum sensing activity. The most potent Fruit Ethyl acetate (FEE) extract at non-inhibitory concentrations was found to interrupt both short-chain (RhlI/R) and long-chain (LasI/R) QS circuits and other virulence factors (P<0.05) such as elastase, protease, rhamnolipids and pyocyanin levels in P. aeruginosa . Biofilm inhibitory properties of FEE were demonstrated using atomic force microscopy, scanning electron microscope and confocal laser microscope. Caenorhabditis elegans infection model (Paralytic assay) was developed to determine the protective role of FEE by reducing the pathogenicity of P. aeruginosa . Results. The study results suggest that hot crude FEE extract interfered in the QS circuit, leading to comprehensive debilitation of QS-controlled virulence factors. The extract reduced virulence factor production in P. aeruginosa at 4 mg ml-1 concentration whilst paradoxically promoting biofilm formation. Possibly, higher sugar content in the extract promoted clump formation of biofilm architecture by increasing exopolysaccharide production. Moreover, in vivo analysis of bacterial pathogenesis on Caenorhabditis elegans reveals a drastic increase in survival rates in FEE treated worms compared to untreated control. Conclusions. FEE showed promising QS inhibitory activity against P. aeruginosa . In the future, additional purification of crude FEE is required to remove carbohydrates, and pure isolated phytochemicals from FEE could be used as therapeutic agents to control QS-mediated infections in P. aeruginosa .

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Section: Discussionmentioning

confidence: 99%

“…Similarly, Sandasi et al . [70] reported biofilm enhancement by surface adhesion and micro-colonies induction effect of various medicinal plants ( Rosmarinus officinalis, Mentha piperita and Melaleuca alternifolia ) against Listeria monocytogenes in the presence of crude extracts [59, 60].…”

Section: Discussionmentioning

confidence: 99%

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Peerzada

Shah

Kharkar

et al. 2023

Journal of Medical Microbiology

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“…Cassia fistula Linn., a native to Pakistan, India, Myanmar and Sri Lanka, is a flowering plant of the family Fabaceae. It is widely known as Cana fistula, Amaltas, and Golden Shower [18,19]. C. fistula is a good source of naturally occurring biologically active secondary metabolites (anthraquinones, triterpenes, alkaloids, proanthocyanidins, catechins and flavonoids) which are used as dietary supplements, medicines and other valuable commercial products [20].…”

Section: Introductionmentioning

confidence: 99%

“…C. fistula is utilized to cure many ailments in traditional medicinal systems, including pruritus, leukoderma, haematemesis and obesity [21]. The antitussive, antipyretic, antimicrobial, antifungal, inflammation control and antioxidant potential of this plant have been reported [19,22]. The extraction process is a very important aspect to get maximum pharmacological output from a particular plant part.…”

Section: Introductionmentioning

confidence: 99%

Cassia fistula Leaves; UHPLC-QTOF-MS/MS Based Metabolite Profiling and Molecular Docking Insights to Explore Bioactives Role towards Inhibition of Pancreatic Lipase

Aabideen

Mumtaz

Akhtar

et al. 2021

Plants

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The present work was aimed at investigating hydroethanolic leaf extracts of Cassia fistula for their antioxidant and pancreatic lipase (PL) enzyme inhibitory properties. The most active extract was selected to profile the phytoconstituents by UHPLC-QTOF-MS/MS technique. Among the tested extracts, the 80% hydroethanolic extract exhibited the maximum levels of total phenolic and flavonoid contents (TPC and TFC) with a contribution of 201.3 ± 2.6 mg of gallic acid equivalent per gram of extract (GAE/g extract), and 116.3 ± 2.4 mg of rutin equivalent per gram of extract (RE/g extract), respectively. The same extract also showed promising 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and PL inhibitory activity with an IC50 (half maximal inhibitory concentration) of 30.5 ± 2.8 µg/mL and 17.31 ± 1.18 μg/mL, respectively. The phytochemical profiling of 80% hydroethanolic extract confirmed the presence of 23 metabolites of immense medicinal significance. Docking studies were conducted to investigate the potential interactions of compounds identified in the study. The docking study-based binding energy data and the interaction scheme both revealed the possible role of the identified compounds towards PL inhibitor. Moreover, energies of frontier molecular orbitals (FMOs), ionization potentials (IP), electron affinities (EA) and molecular electrostatic potentials (MEP) were also explored. The findings of the current work suggest that C. fistula is a promising natural source of antioxidant and antiobesity agents, which may be exploited to add pharmacological functionalities to food.

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Abstract: Cassia fistula Linn. (Family: Caesalpinaceae

Cited by 2 publications

References 13 publications

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Cassia fistula Leaves; UHPLC-QTOF-MS/MS Based Metabolite Profiling and Molecular Docking Insights to Explore Bioactives Role towards Inhibition of Pancreatic Lipase

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