Protective effect of Calendula officinalis extract against UVB-induced oxidative stress in skin: Evaluation of reduced glutathione levels and matrix metalloproteinase secretion

Fonseca, Yris Maria; Catini, Carolina Dias; Vicentini, Fabiana T. M. C.; Nomizo, Auro; Gerlach, Raquel Fernanda; Fonseca, Maria José Vieira

doi:10.1016/j.jep.2009.12.019

Cited by 133 publications

(105 citation statements)

References 26 publications

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“…Previously, other works had identified that the flowers of C. officinalis contain The HECO showed 33.90 mg/g of total polyphenols, 24.67 mg/g of total flavonoids and 27.30 mg/g of condensed tannins. According to Fonseca et al (2010), the extract of marigold flowers presented amounts of 28.6 mg/g of total polyphenols (gallic acid equivalents), and 18.2 mg/g of total flavonoids (quercetin equivalents). Therefore, the results obtained by the HECO in the present work were higher than those of the literature.…”

Section: Discussionmentioning

confidence: 99%

“…Organic filters can be either natural or synthetic, and are capable of absorbing ultraviolet radiation (Galano et al 2010). One of the current trends in cosmetology is the development of products with the greatest degree of natural ingredients possible.…”

Section: Discussionmentioning

confidence: 99%

“…The flavonoids include quercetin, rutin, narcissin, isorhamnetin and kaempferol (Fonseca et al 2010). Widely used in cosmetic formulations and in solar products due to its moisturizing properties, the extract of the flowers is also used to promote healing, and as an anti-inflammatory (Citadini-Zanette et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Studies conducted with different extracts of calendula have demonstrated anti-tumor, antiinflammatory, healing, and antioxidant properties (Fonseca et al 2010;Agatonovic-Kurstin & Loescher 2013). Also, considering the healing properties attributed to the plant, the angiogenic activity of ethanol extracts, hexanic, and dichlorometanic fractions of C. officinalis L. has been tested, and a positive effect on angiogenesis, inducing neovascularization was observed (Parente et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The antioxidant capacity of C. officinalis L. flower extract has been demonstrated in vitro by the DPPH method, and through UVB radiation exposure on the skin of mice (after oral administration), where skin protection was determined to be caused by glutathione (GSH) depletion prevention (Fonseca et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

Deuschle

Piana

et al. 2015

Rev. bras. plantas med.

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ABSTRACT:The plant Calendula officinalis L. is widely applied due to its medicinal properties, which are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical components in a hydroethanolic extract (HECO) from the leaves of Calendula officinalis L. using UV-VIS spectrophotometry and thin layer chromatography (TLC), as well as to identify and quantify the components related to its antioxidant capacity employing high performance liquid chromatographic (HPLC). The antioxidant capacity evaluation was performed using the DPPH method for superoxide and hydroxyl radicals. The photo-protective capacity was evaluated by UVspectrophotometry in order to determine the in vitro Sun Protection Factor(SPF). The results show the plant's strong antioxidant activity (DPPH and hydroxyl methods), which we believe to be related to the presence of flavonoids (24.67 mg/g), polyphenols (33.90 mg/g), condensed tannins (27.30 mg/g), and the amount of rutin (37.25 mg/g), and quercetin (6.09 mg/g) found during the study. The HECO presented a good antioxidant capacity, most likely due to the polyphenols, flavonoids, and tannins in its contents. However, the obtained SPF of 1.89 ± 0.05 does not allow the plant to be classified as a stand-alone sunscreen, and more studies are needed in order to test its ability to enhance sunscreens in existing cosmetic formulations.Keywords: Calendula officinalis. Marigold. Antioxidant. Phenolic Compounds. Photo-protection. RESUMO:Avaliação fitoquímica e capacidade antioxidante e fotoprotetora in vitro das folhas de Calendula officinalis L. A Calendula officinalis L. é uma planta amplamente utilizada por suas propriedades medicinais, principalmente dermatológica e ornamental. O objetivo deste estudo é avaliar constituintes fitoquímicos do extrato hidroetanólico das folhas de Calêndula officinalis L. (HECO) por espectrofotometria UV-visível e cromatografia em camada delgada (CCD), bem como detectar e quantificar os componentes relacionados com a capacidade antioxidante por Cromatografia Líquida de Alta Eficiência (CLAE). Posteriormente, foi avaliada a capacidade antioxidante pelo método do DPPH, radicais superóxido e hidroxila. Além disso, a capacidade fotoprotetora foi avaliada através de espectrofotometria UV para determinação do Fator de Proteção Solar in vitro (FPS). Os resultados evidenciaram que a planta apresentou uma excelente atividade antioxidante para o método do DPPH e do radical hidroxila, o que pode estar relacionada com a presença de flavonoides (24,67 mg/g), polifenóis (33,90 mg/g) e taninos condensados (27,30 mg/g) e da quantidade de rutina (37,25 mg/g) e quercetina (6,09 mg/g) encontrados neste estudo. O HECO apresentou uma notável capacidade antioxidante, provavelmente devido à presença de polifenóis, flavonoides e taninos. O valor do FPS encontrado (1,89 ± 0,05), não classifica a planta como um protetor solar isolado. No entanto, mais estudos são necessários para testar a capacidade de potencializar filtros solares em formulações cosméticas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%