2015
DOI: 10.1590/1983-084x/14_055
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Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

Abstract: ABSTRACT:The plant Calendula officinalis L. is widely applied due to its medicinal properties, which are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical components in a hydroethanolic extract (HECO) from the leaves of Calendula officinalis L. using UV-VIS spectrophotometry and thin layer chromatography (TLC), as well as to identify and quantify the components related to its antioxidant capacity employing high performance liquid chromatographic (HPLC). The antioxidant… Show more

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Cited by 10 publications
(4 citation statements)
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“…Among the 9 plants in a study conducted by Nunes et al (2018), ethanolic extracts of leaves of L. microphylla showed the highest SPF value of 26.82, and S. galheirensis has the lowest SPF value of 5.40 28 . The aqueous ethanolic extract of C. officinalis L. showed low SPF values of 1.89 29 . Yasmeen and Gupta (2016) reported the methanolic barks, leaves, and fruit extracts of D .…”
Section: Resultsmentioning
confidence: 99%
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“…Among the 9 plants in a study conducted by Nunes et al (2018), ethanolic extracts of leaves of L. microphylla showed the highest SPF value of 26.82, and S. galheirensis has the lowest SPF value of 5.40 28 . The aqueous ethanolic extract of C. officinalis L. showed low SPF values of 1.89 29 . Yasmeen and Gupta (2016) reported the methanolic barks, leaves, and fruit extracts of D .…”
Section: Resultsmentioning
confidence: 99%
“…Deuschle (2015) found alkaloids, terpenoids, saponins, and volatile oils using thin‐layer chromatography, subsequently, they identified 25 mg/g of rutin and 6.09 mg/g of quercetin in the leaves of C. officinalis L. 29 . Raquel et al (2017) identify apigenin, catchehin, epicatechin, and gallic acid in the barks of S. brasiliensis by comparing the retention time of peaks analytical standards using HPLC–DAD analysis 32 .…”
Section: Resultsmentioning
confidence: 99%
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“…Evaluation of phenolic compounds in Calendula plants is an important task, as indicated by the known HPLC protocols found in the scientific literature. To separate target compounds, only RP C18 columns with varying lengths were used, such as 75 mm ProntoSIL-120-5-C18 [ 90 , 114 , 144 ]; 100 mm Phenomenex C18 [ 142 ], Zorbax SB-C18 [ 143 ], and LiChrosorb RP18 [ 100 , 153 ]; 150 mm Luna C18 [ 131 ], SiliaChrom C-18 [ 140 ], Eclipse XDB-C18 [ 146 ], Spherisorb S3 ODS-2 C18 [ 149 ], Zorbax Eclipse Plus C18 [ 151 ], and Aquity UPLC HSS T3 [ 154 ]; 220 mm Aquapore RP-300 [ 152 ]; 250 mm Shim-pack C-18 [ 96 ], Hypersil C18 [ 141 , 147 ], X-Bridge C18 [ 145 ], and Phenomenex C18 [ 148 ]; 300 mm Bondclone C18 [ 133 ]; and 1000 mm Hypersil Gold [ 75 ]. The presence of various eluents requires the frequent use of formic acid [ 75 , 96 , 142 , 150 , 151 ], acetic acid [ 133 , 141 , 148 ], phosphoric acid [ 140 , 146 ] as the polar eluent and methanol [ 133 , 140 ] and acetonitrile [ 96 , 114 , 141 , 142 ] as the non-polar eluent.…”
Section: Separation Of Calendula Metabolites By Gc...mentioning
confidence: 99%