Protective effect of 4′, 5-dihydroxy-3′,6,7-trimethoxyflavone fromArtemisia asiaticaagainst Aβ-induced oxidative stress in PC12 cells

Abstract

Amyloid beta protein (Abeta)-induced free radical-mediated neurotoxicity is a leading hypothesis as a cause of Alzheimer's disease (AD). Abeta increased free radical production and lipid peroxidation in PC12 nerve cells, leading to apoptosis and cell death. The effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica on Abeta induced neurotoxicity was investigated using PC12 cells. Pretreatment with isolated 4',5-dihydroxy-3',6,7-trimethoxyflavone and vitamin E prevented the Abeta-induced reactive oxygen species (ROS). The 4',5-dihydroxy-3',6,7-trimethoxyflavone resulted in concentration-dependant decreased Abeta toxicity assessed by 3-(4, 5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. However, treatment with these antioxidants inhibited the Abeta-induced neurotoxic effect. Therefore, these results indicate that micromolecular Abeta-induced oxidative cell stress is reduced by 4,5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica.

Naringenin from <i>Citrus junos</i> Has an Inhibitory Effect on Acetylcholinesterase and a Mitigating Effect on Amnesia

“…The methanol extract of the ground C. junos with an inhibitory effect on AChE from the PC12 cell was fractionated by ethyl acetate [14]. The ethyl acetate fraction had the highest inhibitory effect on AChE activity ( fig.…”

Antiamnesic Effects of Ethyl Acetate Fraction from Chestnut (Castanea crenata var. dulcis) Inner Skin on Aβ25–35-Induced Cognitive Deficits in Mice

“…18 In brief, cells (10 4 cells/well on 96-well) were treated for 48 h with the indicated concentrations of the ethyl acetate extract of chestnut inner skin or vitamin C. The cells were then treated with or without 200 lM H 2 O 2 for 2 h. At the end of the treatment, cells were incubated in the presence of 50 lM DCF-DA in phosphate-buffered saline. Fluorescence was then quantified using a TECAN SER-NR 94572 fluorometer (San Jose, CA, USA) using 485-nm excitation and 530-nm emission filters.…”

Protective Effect of DetoxifiedRhus vernicifluaStokes on Human Keratinocytes and Dermal Fibroblasts against Oxidative Stress and Identification of the Bioactive Phenolics

“…The cytotoxicity was determined by an MTT reduction assay to obtain the maximal non-toxic concentration of the DRVS extract. 24) MTT is metabolized to an insoluble purple formazan by mitochondrial dehydrogenases which are active only in living cells. The cell viability was therefore assessed from the formation of the purple formazan metabolite which was rendered soluble by adding DMSO.…”