Protective Effect of Detoxified<i>Rhus verniciflua</i>Stokes on Human Keratinocytes and Dermal Fibroblasts against Oxidative Stress and Identification of the Bioactive Phenolics

Liu, Chunshan; Nam, Tae Gyu; Han, Min‐Woo; Ahn, Soo-Mi; Choi, Han Seok; Kim, Tae Young; Chun, Ock K.; Koo, Sung I.; Kim, Dae‐Ok

doi:10.1271/bbb.130236

Cited by 29 publications

(23 citation statements)

References 29 publications

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“…According to a previous report, RVS extracts detoxified via heating methods show high gallic acid contents [17]. This is consistent with our finding that DRVE, an allergen-free RVS extract detoxified by heating to a high temperature, possesses the highest gallic acid content.…”

Section: Discussionsupporting

confidence: 93%

“…Since then, the types of functional foods containing detoxified RVS extracts have been steadily increasing on a yearly basis. Detoxification methods include the removal of allergens from RVS using solvents [15], electron beam radiation [16], high temperature [17], and microorganisms [9, 13]. However, RVS extracts detoxified by solvents and irradiation are not suitable for use in food.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the main components and bioactivity of Rhus verniciflua Stokes extracts by different detoxification processing methods

Lee

Kim

et al. 2018

BMC Complement Altern Med

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BackgroundRhus verniciflua Stokes is an Asian tree species that is used as a food supplement and traditional medicine in Korea. However, its use is restricted by its potential to cause allergy. Thus, allergen-free R. verniciflua extracts are currently being marketed as a functional health food in Korea. In the present study, three different allergen-free R. verniciflua extracts (DRVE, FRVE, and FFRVE) were produced by detoxification of R. verniciflua, and their properties and constituents were compared.MethodsThe main components and properties (antibacterial, antioxidant, anticancer, and hepatic lipogenesis inhibitory effects) of the three allergen-free extracts were compared. Moreover, the major phenolic constituents of R. verniciflua, including gallic acid, fustin, fisetin, and quercetin, were analyzed in the three extracts.ResultsDRVE was superior to the two other extracts with regard to antioxidant activity, while FRVE was superior with regard to antimicrobial activity and suppression of hepatic lipogenesis. FRVE exhibited lipid-lowering effects by lowering sterol regulatory element-binding protein 1 and triglyceride levels, and promoting the activation of peroxisome proliferator-activated receptor and AMP-activated protein kinase in an in vitro model of non-alcoholic fatty liver.ConclusionsOverall, our findings demonstrate various differences among the three extracts. This suggests that functional and bioactive compounds present in R. verniciflua could be altered by the detoxification process, and this property could be considered in the development of functional health foods in the future.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Comparison of the main components and bioactivity of Rhus verniciflua Stokes extracts by different detoxification processing methods

Lee

Kim

et al. 2018

BMC Complement Altern Med

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“…Butein effect on ROS generation has been investigated in various physiological and pathological conditions with different cell types [14,21,29-36]. While butein has been reported to induce ROS generation in MDA-MB-231 breast cancer cells [14], its effect on ROS generation is inconsistent in different biological conditions [3,8,15,20,30,32].…”

Section: Introductionmentioning

confidence: 99%

“…Butein increased ROS level in particular cell types or experimental conditions, resulting in apoptotic cell death [14,21,31]. However, butein inhibited ROS level increased by particular environmental cues such as ethanol, H 2 O 2 and TNF-α [29,30,32-36]. Furthermore, it has been revealed that ROS regulates breast cancer progression [37,38].…”

Section: Introductionmentioning

confidence: 99%

Butein suppresses breast cancer growth by reducing a production of intracellular reactive oxygen species

Cho

Woo

2014

J Exp Clin Cancer Res

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BackgroundButein has various functions in human diseases including cancer. While anti-cancer effects of butein have been revealed, it is urgent to understand a unique role of butein against cancer. In this study, we demonstrate that butein inhibition of reactive oxygen species (ROS) production results in suppression of breast cancer growth.MethodsDifferent breast cancer cell lines were treated with butein and then subjected to cell viability and apoptosis assays. Butein-sensitive or -resistant breast cancer cells were injected into mammary fat pads of immunocompromised mice and then butein was injected. Breast cancer cells were categorized on the basis of butein sensitivity.ResultsButein reduced viabilities of different breast cancer cells, while not affecting those of HER2-positive (HER2+) HCC-1419, SKBR-3 and HCC-2218 breast cancer cells. Butein reduction of ROS levels was correlated with apoptotic cell death. Furthermore, butein reduction of ROS level led to inhibitions of AKT phosphorylation. N-acetyl-L-cysteine (NAC), a free radical scavenger, also reduced ROS production and AKT phosphorylation, resulting in apoptotic cell death. In contrast, inhibitory effects of both butein and NAC on ROS production and AKT phosphorylation were not detected in butein-resistant HER2+ HCC-1419, SKBR-3 and HCC-2218 cells. In the in vivo tumor growth assays, butein inhibited tumor growth of butein-sensitive HER2+ BT-474 cells, while not affecting that of butein-resistant HER2+ HCC-1419 cells. Moreover, butein inhibition of ROS production and AKT phosphorylation was confirmed by in vivo tumor growth assays.ConclusionsOur study first reveals that butein causes breast cancer cell death by the reduction of ROS production. Therefore, our finding provides better knowledge for butein effect on breast cancer and also suggests its treatment option.

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“…When keratinocytes were treated with RVE, the polyphenols were shown to increase resistance to oxidative stress and matrix metalloprotease (MMP)-13 expression, which are associated with aging, and showed a decrease in dermal fibroblasts. These results indicate the possible use of RVE as a substance to prevent the skin aging process8. While some in vitro studies related to aging have reported the suppression of MMP-13 due to the antioxidant effects of the RVE, there have been no in vivo studies presenting the anti-aging effects of RVE on the skin.…”

Section: Introductionmentioning

confidence: 89%

The Effect of Rhus verniciflua Stokes Extracts on Photo-Aged Mouse Skin

et al. 2017

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BackgroundRhus verniciflua Stokes (RV) has traditionally been used in Korea as an indigenous food (Rhus chicken soup) and as an herbal medicinal plant. While the anticancer, antimicrobial, and anti-inflammatory properties of RV have been actively studied in the medical field, its antioxidant effects in the skin that resist the reactive oxygen species in keratinocytes and fibroblasts is less understood.ObjectiveWe designed to evaluate the effects of R. verniciflua Stokes extract (RVE) on the photo-aged skin by an in vitro experiment using human fibroblasts and an in vivo experiment using a photo-aged murine model.MethodsFor the in vitro experiments, human fibroblasts irradiated with ultraviolet (UV) B were treated with RVE or vehicle, and the growth levels and the expression level of type 1 procollagen were compared. For the in vivo experiment, photo-aged mice irradiated with UVB and UVA were administered drinking water with or without RVE, and histological changes and the expression level of type 1 procollagen and matrix metalloprotease (MMP)-13 were compared.ResultsIn vitro experiments using fibroblasts irradiated with UVB showed that RVE promoted growth and significantly increased the expression of type 1 procollagen as compared to the control group. In the photo-aged mice, RVE increased collagen content in the dermis and promoted the synthesis of type 1 procollagen without any visible decrease in MMP-13 as compared to control group.ConclusionIn addition to the previously reported antioxidant effects of RVE, oral intake of RVE effectively inhibited photo-aging in hairless mice by enhancing collagen synthesis.

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Protective Effect of DetoxifiedRhus vernicifluaStokes on Human Keratinocytes and Dermal Fibroblasts against Oxidative Stress and Identification of the Bioactive Phenolics

Cited by 29 publications

References 29 publications

Comparison of the main components and bioactivity of Rhus verniciflua Stokes extracts by different detoxification processing methods

Comparison of the main components and bioactivity of Rhus verniciflua Stokes extracts by different detoxification processing methods

Butein suppresses breast cancer growth by reducing a production of intracellular reactive oxygen species

The Effect of Rhus verniciflua Stokes Extracts on Photo-Aged Mouse Skin

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