Protective effect of 3,5‑dicaffeoyl‑epi‑quinic acid against UVB‑induced photoaging in human HaCaT keratinocytes

Abstract

derivatives of caffeoylquinic acid (cQa) have been studied and reported as potent bioactive molecules possessing various health benefits including antioxidant and anti-inflammatory activities. In the present study, the protective effect of 3,5-dicaffeoyl-epi-quinic acid (dceQa) isolated from Atriplex gmelinii on uVB-induced damages was investigated in human HaCaT keratinocytes. The effect of DCEQA against UVB-induced oxidative stress-mediated damages was determined measuring its ability to alleviate uVB-induced elevation of oxidative stress, proinflammatory response and antioxidant enzyme suppression through nuclear factor-like 2 (Nrf2). Treatment with DCEQA hindered the generation of intracellular reactive oxygen species. Increased levels of proinflammatory cytokines TNF-α, coX-2, il-6 and il-1β following UVB exposure were suppressed by the introduction of DCEQA. Additionally, DCEQA upregulated the mRNA and protein expression of antioxidant enzymes superoxide dismutase-1 and heme oxygenase-1 which were inhibited under UVB irradiation. Antioxidant enzyme regulation transcription factor Nrf2 was also upregulated in the presence of DCEQA. These results suggest that dceQa prevents photoaging via protection of keratinocytes from uVB irradiation by ameliorating the oxidative stress and pro-inflammatory response.

Antiphotoaging Effects of 3,5‐Dicaffeoyl‐epi‐quinic Acid via Inhibition of Matrix Metalloproteinases in UVB‐Irradiated Human Keratinocytes

“…e current study, therefore, employed an in vitro immortalized keratinocyte model, HaCaT cells, in order to investigate potential properties of DCEQA against UVB-mediated photoaging. In a previous study, DCEQA was found to protect keratinocytes against UVB irradiation via regulation of antioxidant defense mechanism: scavenging the ROS and stimulating the antioxidant enzyme production via Nrf2 pathway [22]. e current study added more insight into the suggested antiphotoaging effect of DCEQA by showing that treatment with DCEQA not only protected cells from UVB-induced cytotoxicity but also suppressed MMP expression while increasing the type I procollagen content.…”

“…Most of the compounds isolated from these natural products showed strong antioxidant effect and inhibited the enzymatic activities of MMPs in various occasions. e ROS scavenging effect of DCEQA was previously reported against UVB-induced elevation of the oxidative stress in human keratinocytes [22]. Chiang et al [30] showed that Coffea arabica extract and its major components, caffeic and chlorogenic acid, suppressed MMP expression via downregulation of MAPK pathway.…”

“…Up to date, CQA and CQA-based compounds have been exhibited to show important health benefits such as antioxidant [16], antihistaminic [17], antibacterial [18], antiviral [19], and anticancer [20] activities. As a part of our continuous effort to develop natural products with bioactivities, 3,5-dicaffeoyl-epi-quinic acid (DCEQA), a bioactive derivative of CQA, has been shown to possess antiobesity [21] and photoprotective antioxidant abilities [22]. e current study is aimed at evaluating the photoprotective effect of DCEQA against UVB-induced changes in skin cells and analyzing its possible mechanism of action for antiphotoaging potential.…”

Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro

“…DEQA was isolated, as reported earlier [ 21 ], and its structure is given in Figure 1 A. The cytotoxic effect of DEQA on HDF was evaluated by MTT test.…”

“…Caffeoylquinic acid and its derivatives have been reported to show antioxidant [ 17 ], antibacterial [ 18 ], antiviral [ 19 ] and antiobesic effects [ 20 ]. Previous studies have demonstrated the protective effect of 3,5-dicafeoyl-epi-qinic acid (DEQA) in UVB-irradiated keratinocytes [ 21 , 22 ]. However, its applicability for treating UVA-induced photoaging in human dermal fibroblast cells (HDFs) has not been reported.…”