Antiphotoaging Effects of 3,5-Dicaffeoyl-epi-quinic Acid via Inhibition of Matrix Metalloproteinases in UVB-Irradiated Human Keratinocytes

Abstract

UVB exposure is one of the causes of several skin complications including but not limited to premature aging, wrinkle formation, and hyperpigmentation. UV-induced skin aging is called photoaging, and oxidative stress-induced overexpression of matrix metalloproteinases (MMPs) is the main reason behind the photoaging-mediated collagen degradation. Natural origin inhibitors of MMPs are regarded as a promising approach to prevent or treat photoaging. Therefore, the present study investigated the protective effects of 3,5-dicaffeoyl-epi-quinic acid (DCEQA) in human HaCaT keratinocytes against UVB irradiation-related dysregulation of MMPs. Changes in the mRNA and protein expression and release of MMP-1, -2, and -9 were observed after UVB irradiation with or without DCEQA treatment. In addition, the effect of DCEQA on the activation of p38, JNK, and ERK MAPKs was analyzed. Treatment of UVB-irradiated HaCaT cells with 10 μM DCEQA significantly suppressed the overexpression of both mRNA and protein of MMP-1, -2, and -9 while slightly increasing the diminished type I procollagen production. UVB-induced activation of MAPKs was also ameliorated by DCEQA treatment in a dose-dependent manner. Results indicated that DCEQA treatment was able to protect keratinocytes from UVB-induced photoaging by inhibiting the stimulated production of MMPs and the related decrease in collagen production. It was suggested that DCEQA downregulated the collagen degradation via inhibition of MAPK activation, which resulted in decreased MMP activity.

Circ-140/chi-miR-8516/STC1-MMP1 Regulates αs1-/β-Casein Secretion and Lipid Formation in Goat Mammary Epithelial Cells

“…Furthermore, it is interesting to note that STC1 might not be a terminal regulator of involution, because MMP1 exhibits a strong signal of immunohistochemical localization in the mammary gland during late involution [ 6 ] and is regulated by STC1 [ 22 ]. Studies have shown the mediating role of MAPK activation in MMP1 upregulation [ 10 , 11 , 12 ], and we speculated that the indirect regulation of MMP1 by chi-miR-8516 might be achieved by the modulation of MAPK activation.…”

“…It is reported that milk produced by STC1 -overexpressing mother mice causes growth retardation in young mice [ 9 ]. Studies have shown the mediating role of MAPK activation in MMP1 upregulation [ 10 , 11 , 12 ], and we speculated that the indirect regulation of MMP1 by chi-miR-8516 might be achieved by the modulation of MAPK activation. Circular RNAs are a class of endogenous noncoding RNAs generated by non-classical alternative splicing that act as miRNA sponges and inhibit the process of miRNA-targeting genes [ 13 , 14 , 15 ].…”

The Modulatory Influence of Plant-Derived Compounds on Human Keratinocyte Function

“…Studies have examined the protective roles of single compounds on epidermal cells, including 3,5-dicaffeoyl-epiquinic acid obtained from Atriplex gmelinii [167], quercetin 3,7-dimethyl ether 40-glucoside from Nymphoides indica [163], youngiasides A and C from Youngia denticulatum [146], ixerisoside A from Ixeris dentata [168], α-, β-, γ-mangostins and gartanin from Garcinia mangostana [169]. Of these, 3,5-dicaffeoyl-epiquinic acid downregulates the expression of MMP-1, -2 and -9, whereas α-, β-, γ-mangostins and gartanin downregulate the expression of MMP-1 and -9.…”

Exploration of photoprotective and antibiotic activity of 20 wild Polypodiaceae ferns from Costa Rica

“…Commercial aqueous extract of the leaves of P. aureum (marketed as Fernblock®, Fernplus®, Fernmed®, and Fernage®) contain a standardized 0.6-1.3% total phenolic compounds concentration and 0.4-0.9% quinic acid concentration 4 . Mechanisms of action of these commercial products involve free radical scavenging and regulation of several genes, such as metalloproteinases 5 , tyrosinase inhibition 6 , and others. Also, natural phenolic compounds are known for absorbing UV radiation, similar to synthetic compounds, such as homosalate or dibenzalacetone do so.…”