Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro

Abstract

Cutaneous aging is divided into intrinsic and exogenous aging correspondingly contributing to the complex biological phenomenon in skin. Intrinsic aging is also termed chronological aging, which is the accumulation of inevitable changes over time and is largely genetically determined. Superimposed on this intrinsic process, exogenous aging is associated with environmental exposure, mainly to ultraviolet (UV) radiation and more commonly termed as photoaging. UV-induced skin aging induces increased expression of matrix metalloproteinases (MMPs) which in turn causes the collagen degradation. Therefore, MMP inhibitors of natural origin are regarded as a primary approach to prevent or treat photoaging. This study investigated the effects of 3,5-dicaffeoyl-epi-quinic acid (DEQA) on photoaging and elucidated its molecular mechanisms in UVA-irradiated human dermal fibroblasts (HDFs). The results show that treatment with DEQA decreases MMP-1 production and increases type I collagen production in UVA-damaged HDFs. In addition, treatment of UVA-irradiated HDFs with DEQA downregulates MMP-1, MMP-3 and MMP-9 expression via blocking MAPK-cascade-regulated AP-1 transcriptional activity in UVA-irradiated HDFs. Furthermore, DEQA relieves the UVA-mediated suppression of type I procollagen and collagen expression through stimulating TGF-β/Smad signaling, leading to activation of the Smad 2/3 and Smad 4 nuclear translocation. These results suggest that DEQA could be a potential cosmetic agent for prevention and treatment of skin photoaging.

Optimization of Cyclodextrin-Assisted Extraction of Phenolics from Helichrysum italicum for Preparation of Extracts with Anti-Elastase and Anti-Collagenase Properties

“…For example, one in vitro study on 3,5- O -dicaffeoylquinic acid isolated from Artemisia lavandulaefolia , found that it exhibited anti-rosacea effects [ 65 ]. Additionally, 3,5- O -dicaffeoylquinic acid demonstrated an anti-photoaging effect against skin damage induced by radiation in the UVA region (400–315 nm) and protected human dermal fibroblasts in vitro [ 66 ]. As 3,5- O -dicaffeoylquinic acid was the most prevalent compound in both extracts, this confirmed the potential of the prepared extracts for use in cosmetics.…”

“…However, as the essential oil components are highly lipophilic, it is not likely that their concentration in the CD-aqueous extracts would be sufficient to be solely responsible for the observed effects. It is interesting to note that 3,5- O -dicaffeoylquinic acid decreases interstitial collagenase production and increases type I collagen production in UVA-damaged human dermal fibroblasts [ 66 ].…”

Chemical Composition and Protective Effect of Young Barley (Hordeum vulgare L.) Dietary Supplements Extracts on UV-Treated Human Skin Fibroblasts in In Vitro Studies

“…The antiphotoaging activity showed derivates of detected quinic acid-3,5-Dicaffeoyl-epi-quinic acid. This effect was tested against skin damage induced by UVA and UVB radiation in human fibroblasts [ 85 , 86 ]. Published studies suggested that inositol may be effective in patients with moderate acne [ 87 ].…”

Protective effects of quercetin-3-glucosyl-(1-2)-rhamnoside from Schizophragma hydrangeoides leaves on ultraviolet A-induced photoaging in human dermal fibroblasts

“…Skin collagen is responsible for ECM structure and contributes to skin elasticity [23]. Therefore, we performed ELISA and western blotting to investigate the effects of Q-3-GR on procollagen type I production and protein expression upon UVA-induced photoaging.…”