Protection of shrimp (Penaeus chinensis) against white spot syndrome virus (WSSV) challenge by double-stranded RNA

Penaeus monodon is protected against gill- associated virus by muscle injection but not oral delivery of bacterially expressed dsRNAs

“…The suite of dsRNAs was selected to include 2 targeted at the 5'-terminus and near 5'-terminus of the GAV genome to preferentially destroy sequence motifs needed for polymerase binding to perpetuate GAV genome replication. Moreover, whilst the genes encoding GAV structural proteins might be seen as reasonable targets to mount anti-viral RNAi responses, as found to be the case with WSSV (Kim et al 2007, Sarathi et al 2008 and other shrimp viruses, targeting such genes in okaviruses is expected to be inefficient. This inefficiency stems from such dsRNAs interacting not only with the genomic-length (+) and (-) sense RNAs, but also with either or both of the 2 (+) sense 3'-coterminal sub-genomic ssRNAs from which the virion structural proteins are translated, as well as their (-) sense subgenomic ssRNA counterparts, both of which are transcribed in higher abundance than the genomic-length RNAs , Sittidilokratna et al 2008.…”

Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477 Gene against WSSV Infection in Penaeus monodon

“…Protective efficacies of various dsRNA targeting structural and nonstructural protein coding genes against WSSV infection is evidenced by several studies (Kim et al, 2007;Robalino et al, 2004Robalino et al, , 2005Tirasophon et al, 2005;Yodmuang et al, 2006). Structural gene VP26 is one among the major component of the virus envelope (Zhang et al, 2002;Tang et al, 2007) involved in thecytoplasmic transport of the nucleocapsid by interacting with actin or cellular actin-binding proteins ( Xie and Yang 2005 ).…”

Characterization of White Spot Syndrome Virus Envelope Protein VP51A and Its Interaction with Viral Tegument Protein VP26

“…In the case of white spot syndrome virus (WSSV) (genus Whispovirus, family Nimaviridae) (37), a double-stranded DNA virus that has caused severe mortality and huge economic losses to the shrimp farming industry globally for more than a decade (5,19), proteomic methods have helped to identify a total of 58 structural proteins, over 30 of which are currently recognized as envelope proteins (13,31,44,47). Some of the WSSV envelope proteins involved in shrimp infection have been identified (12,14,34,36,41,43), and these envelope and other WSSV structural proteins have been used in various studies, including RNA interference-based gene knockdown to silence viral structural-protein gene expression (8,27,45), DNA and protein vaccination to elevate host immunity (25,29,36,39), and antibody neutralization techniques that neutralize the virus by preventing envelope proteins from interacting with host cell receptors (12,34,41,43).…”