Protection of Rats Against Dental Caries by Passive Immunization With Hen-egg-yolk Antibody (IgY)

Abstract

Hen-egg-yolk antibody (IgY) was prepared against Streptococcus mutans MT8148 serotype c that was cultivated in medium containing sucrose, and it was used in passive caries-immunity studies. Specific pathogen-free rats infected with S. mutans MT8148 (c) and fed with a cariogenic diet containing more than 2% immune yolk powder developed significantly lower caries scores than did the ones infected with the same strain and fed with a diet containing only control yolk powder obtained from non-immunized hens. Similar results were obtained in an experiment with rats infected with S. mutans JC-2 (c) strain. Rats provided a diet supplemented with 0.5% immune water-soluble protein fraction containing S. mutans-specific IgY and challenged with S. mutans MT8148 exhibited significantly fewer caries lesions, compared with control rats on the normal diet.

Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

“…Research conducted by Paau and Rong-Jiang [21] in 2006 showed that the IgY-anti-S. mutans toothpaste decreased the percentage of S. mutans among the total anaerobic bacteria found in both saliva and plaque samples. Similar to previous research, Otake et al [22] used anti-S. mutans whole-cell IgY in the form of frozen egg yolk in the rat diet. However, the colony count of S. mutans was not significantly different after this intervention.…”

“…Wen et al [27] reported that IgY was capable of impeding glucan synthesis by S. mutans and S. sobrinus. However, several of these studies did not use gel as a clinical application medium, instead relying on mouthwash or food and toothpaste [21,22]. Compared with mouthwash, pure IgY is dissolved in 10 ml of water [20].…”

“…The coating method was conducted once per day in the labial buccal part of the rats' teeth ( Figure 1). A plaque swab sample was taken from the anterior teeth for the colony forming unit (CFU) test [22]. To test the anti-cariogenic activity of IgY antibodies in vivo, the experimental rat caries model was used.…”

Functional Proteins and Peptides of Hen’s Egg Origin

“…Administration of IgY against S. mutans CA-GTase specifically inhibited insoluble glucansynthesizing CA-GTase, resulting in a significant reduction in the development of dental caries. Otake et al (1991) reported that anti-S. mutans CA-GTase IgY suppressed development of dental carries in rat model [42]. Hatta et al (1997) reported that the effectiveness of IgY with specificity to S. mutans prevented the colonization of mutans streptococci in the oral cavity of humans [43].…”

Antimicrobial and Antihaemolytic Activities of Crude Extracts of Some Commonly Used Tea and Coffee in Nigeria

“…Regular consumption of Tea and Coffee have been reported by Matheson et al (2011) to be associated with a likelihood of lower MRSA nasal carriage and thereby recommending it as a new, safe, inexpensive and easily accessible method to decrease MRSA nasal carriage. Tea contains substances with antimicrobial potentials such as caffeine, theobromine, xanthine and tannic acid (Elvin-Lewis et al, 1980;Otake et al, 1991). Toda et al (1989) reported the antimicrobial effect of coffee on Staphylococcus aureus, Salmonella typhi, Shigella dysentariae, Vibrio cholera, Vibrio parahaemolyticus and Yersinia enterolitica and attributed the bactericidal effect to the presence of tannic acid.…”