Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

Bachtiar, Endang W.; Afdhal, Anggraeni; Meidyawati, Ratna; Soejoedono, Retno Damajanti; Poerwaningsih, Erni

doi:10.1556/030.63.2016.2.2

Cited by 16 publications

(13 citation statements)

References 26 publications

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“…Os resultados obtidos identificaram que a IgY reduziu o potencial do S. mutans em formar biofilme e minimizou a interação com outras bactérias comumente encontradas na superfície dentária, tal qual a Actinomyces viscosus e a Lactobacillus spp. (Bachtiar et al 2016).…”

Section: Discussionunclassified

Atividade antimicrobiana e antibiofilme da imunoglobulina Y: uma revisão de literatura

Silva

Machado

2020

RSD

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A imunoglobulina Y (IgY), termo utilizado para referir-se ao principal anticorpo sérico em galinhas e presente em grande quantidade na gema do ovo, tem se mostrado altamente relevante no meio científico nas últimas décadas. Estudos recentes têm avançado ao estudar a relação da imunoglobulina com atuações antimicrobiana e antibiofilme. Sendo assim, o objetivo do presente estudo é analisar aspectos estruturais e funcionais da imunoglobulina Y bem como sua atividade antimicrobiana e antibiofilme. Para isso, foi realizada uma busca nas bases de dados PubMed/MEDLINE, Cochrane e EMBASE, utilizando os descritores MeSH/DeCS “Dental caries”, “IgY” e seus derivados, associados entre si pelo operador booleano “AND” e adaptados para cada base de dados. Além disso, foi feita uma busca complementar no Google Acadêmico e nas referências dos artigos selecionados. Após uma leitura crítica de títulos e resumos, 10 estudos foram selecionados. A revisão inclui estudos publicados nos últimos 20 anos. A imunoglobulina Y além de apresentar um potencial uso em tratamentos odontológicos, mostrou-se eficiente quanto à redução de patógenos como a Salmonella spp e Pseudomonas aeruginosa. Na cavidade oral, a imunoglobulina apresentou atuação seletiva para com a bactéria Streptococcus mutans. Entretanto, não há um consenso na literatura sobre a ação da IgY na candidíase, doenças nutricionais e metabólicas, neoplasias, amigdalite e o resfriado comum. Assim sendo, a imunoglobulina Y apresenta uma boa atuação antimicrobiana in vitro e in vivo em bactérias do biofilme, porém são necessários mais estudos a fim de elucidar sua ação contra outras bactérias e a aplicabilidade clínica da mesma.

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Section: Discussionunclassified

Atividade antimicrobiana e antibiofilme da imunoglobulina Y: uma revisão de literatura

Silva

Machado

2020

RSD

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“…To our knowledge, this is the first study to explore the effect of nicotine on the cariogenic potential of S. mutans in vivo . Regarding the sample sizes ranged from 6 to 10 rats for each group in most caries studies,29, 30, 31, 32, 33, 34 we took 16 rats and divided them into two groups (n = 8). The results of our study provide striking evidence that nicotine promotes the attachment of S. mutans to dental surface and the development of caries in vivo , and it appears to be a promotion factor for the development of dental caries.…”

Section: Discussionmentioning

confidence: 99%

Nicotine is a risk factor for dental caries: An in vivo study

Liu

Zhou

et al. 2018

Journal of Dental Sciences

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Background/purpose Streptococcus mutans is an important pathogen in the development of dental caries. Many studies have focused on the relationship between nicotine and S. mutans in vitro . The aim of this study was to investigate the effect of nicotine on the growth of S. mutans and its cariogenic potential in vivo . Materials and methods Sixteen male Specific-pathogen-free Wistar rats were divided into 2 groups (nicotine-treated and nicotine-untreated group) and infected with S. mutans . The S. mutans suspension was treated with 1 mg/mL nicotine in the nicotine-treated group. The Keyes method was used to evaluate sulcal caries of rats, and dental plaque on molar teeth was observed by scanning electron microscopy (SEM). Results Incidence of sulcal caries was higher in nicotine-treated group compared to nicotine-untreated group (42.7 ± 1.7 vs 37.3 ± 4.9, P = 0.009). Severity of caries increased with nicotine treatment. The slightly dentinal caries scores and moderate dentinal caries scores were higher in the presence of nicotine (P < 0.001). Increased number of S. mutans cells attached to dental surface was observed under SEM in the nicotine-treated group. Conclusion Nicotine would promote the attachment of S. mutans to dental surface, and further increase the incidence and severity of dental caries. Therefore, nicotine might be a risk factor for smoking-induced caries.

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“…The HRP-conjugated anti-rabbit IgG was discarded, and the plate was washed three times with a washing buffer, followed by the addition of 50 µL of TMB (3,3',5,5'tetramethylbenzidine, Sigma-Aldrich; St. Louis, MO), and the plate was incubated for 5 minutes at room temperature. Then, 50 μL of stop solution (1 N sulfuric acid) was added, followed by measuring the optical density at 450 nm in the ELISA reader (AccuReader, M965/M965+; Metertech, Taipei, Taiwan) [25,26].…”

Section: Elisamentioning

confidence: 99%

S. mutans Serotype c, C. albicans, Oral Hygiene, and Decayed, Missing, and Filled Teeth in Early Childhood Caries

Rahmah¹,

Bachtiar²,

Gultom³

et al. 2020

TODENTJ

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Purpose: The purpose of this study was to determine the quantity of Streptococcus mutans serotype c and Candida albicans antigens from dental plaque and their relationship with the Oral Hygiene Index Score (OHI-S) and Decayed, Missing, and Filled Teeth (DMFT) in Early Childhood Caries (ECC) and Severe Early Childhood Caries (S-ECC). Materials and Methods: This was a cross-sectional study, including 5 ECC and 32 S-ECC subjects. ECC was classified as a DMFT score < 4, and S-ECC was classified as a DMFT score ≥ 4 at 71 months of age. S. mutans serotype c and C. albicans antigens from dental plaque of ECC and S-ECC patients were measured using enzyme-linked immunosorbent assays. The optical density of S. mutans serotype c and C. albicans antigens was determined at 450 nm. Correlations between S. mutans serotype c and C. albicans antigens and OHI-S in ECC and S-ECC were analyzed. Results: There were no significant differences in S. mutans serotype c and C. albicans antigens between ECC and S-ECC patients or between patients with moderate and good OHI-S scores. There was a negative correlation between S. mutans serotype c and C. albicans antigens in ECC patients (r = -0,62; p < 0.05). Conclusion: The increase in C. albicans suppresses S. mutans serotype c growth in ECC but not in S-ECC. The findings of this study could be used to discover the molecular mechanisms involved in bacterial-fungal relationships and the contribution of new technologies to reduce ECC.

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Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

Cited by 16 publications

References 26 publications

Atividade antimicrobiana e antibiofilme da imunoglobulina Y: uma revisão de literatura

Atividade antimicrobiana e antibiofilme da imunoglobulina Y: uma revisão de literatura

Nicotine is a risk factor for dental caries: An in vivo study

S. mutans Serotype c, C. albicans, Oral Hygiene, and Decayed, Missing, and Filled Teeth in Early Childhood Caries

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