S. mutans Serotype c, C. albicans, Oral Hygiene, and Decayed, Missing, and Filled Teeth in Early Childhood Caries

Rahmah, Nurul; Bachtiar, Boy M.; Gultom, Ferry Pergamus; Soejoedono, Retno Damajanti; Bachtiar, Endang Winiati

doi:10.2174/1874210602014010731

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…Recent studies in vitro have shown that C.albicans prevent caries [30,31]. Although OHI-S showed no correlation with candida carriage in school children, a similar result was also done in preschool children [32].…”

Section: Discussionmentioning

confidence: 80%

Association between Candida species and caries index in children

Najeeb¹,

Muhammad²,

Mustafa³

2023

djm

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Background: candida species is one of the most important opportunistic oral fungal flora that contributed to oral candidal infections. Recently some studies indicate the association between increased caries incidence in children with oral candidal carriage. Objective: To candida species is one of the most important opportunistic oral fungal flora that contributed to oral candidal infections. Recently some studies indicate the association between increased caries incidence in children with oral candidal carriage. Patients and Methods: Sixty-one children with ages 6 to 12 years , DMFT, dmft, and OHI-S were measured and oral swabs were tested on three different surfaces (lingual, occlusal, and buccal) then cultured on SDA as a primary medium, and selective medium on CHROMagar, also germ tube test was done and for more precision, pure colonies were identified up to species based on their carbohydrate assimilation pattern by the VITEK 2 compact Results: 22 (36.1%) children with candida carriage, C.albicans 12, (54%), C.dubliniensis 4,( 18.18%), C.krusei 4, (18.18%), C. tropicalis 2, ( 9.1%). There was no association between candidal species in the oral cavity and caries index (DMFT, dmft). Conclusion: Non-significant weak negative correlation appeared between candidal species in the oral cavity and caries index in children. The most predominant candidal species was C.albicans.

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Section: Discussionmentioning

confidence: 80%

Association between Candida species and caries index in children

Najeeb¹,

Muhammad²,

Mustafa³

2023

djm

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“…In this study, paraffin-embedded tissue sections of the rat tongue were prepared and probed with the biotinylated aptamer (Ca-apt-1). For comparison, the paraffin-embedded tissue sections were also probed with PcAb 12 as a standard control. The results were further verified by light microscopy, and all images were edited for brightness and contrast by using Adobe Photoshop 7.0.…”

Section: Resultsmentioning

confidence: 99%

“…7 11 As a positive control for the immunohistochemistry assay, a rabbit polyclonal antiserum, anti- C. albicans (ATCC 10231), obtained from the Faculty of Veterinary IPB-University, Bogor, Indonesia was used. 12 This polyclonal antibody was purified by using Antibody Purification Kits (New England BioLabs, Massachusetts, United States) according to the manufacturer's instructions. A secondary antibody staining kit, including horseradish peroxidase (HRP)-conjugated horse anti-rabbit IgG antibody and DAB peroxidase substrate solution for color development, was purchased from GBI Labs (Golden Bridge International).…”

Section: Methodsmentioning

confidence: 99%

Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue

et al. 2021

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Objective This study aimed to validate the use of Ca-apt-1, an RNA aptamer, that we generated previously as a probe for immunostaining of Candida albicans in rat tongue paraffin-fixed tissue sections Material and Methods The performance of Ca-apt-1 as a detector molecule was compared with that of anti-C. albicans polyclonal antibody (PcAb), which was used as a positive control. Immunostaining images were visualized by light microscopy and were analyzed by using ImageJ software. Results Microscopic results demonstrated that Ca-apt-1 specifically recognized and immunostained C. albicans cells of rat tongue candidiasis, with a specificity comparable to that of PcAb. ImageJ analysis showed that the area (pixels) detected by Ca-apt-1 was wider than that detected by the antibody. This indicates that the binding affinity of Ca-apt-1 toward C. albicans was better than that of PcAb on paraffin-embedded tissues. Conclusion This study demonstrates that Ca-apt-1 can be used as a probe for immunostaining of fixed tissue sections for oral candidiasis diagnosis.

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S. mutans Serotype c, C. albicans, Oral Hygiene, and Decayed, Missing, and Filled Teeth in Early Childhood Caries

Cited by 2 publications

References 26 publications

Association between Candida species and caries index in children

Association between Candida species and caries index in children

Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue

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