Protection of enzymes from photodegradation by entrapment within alumina

Shapovalova, Olga E.; Lévy, David; Avnir, David; Vinogradov, Vladimir V.

doi:10.1016/j.colsurfb.2016.07.020

Cited by 15 publications

(6 citation statements)

References 34 publications

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“…In our previous papers, we showed a promising approach to the creation of composite materials based on alumina and biomolecules by direct entrapment of the proteins within the sol-gel derived boehmite matrix (AlOOH) 25–27 . In this approach, the protein is immobilized in the xerogel boehmite cage during the room-temperature sol-gel transition 28,29 . It was shown, that it is possible to obtain systems both with complete immobilization of biomacromolecules and partial release of the proteins depending on the ratio of components and environmental conditions 26,27 .…”

Section: Introductionmentioning

confidence: 99%

“…It was shown, that it is possible to obtain systems both with complete immobilization of biomacromolecules and partial release of the proteins depending on the ratio of components and environmental conditions 26,27 . The entrapped proteins tightly interact with the walls of the ceramic matrix, which leads to an increase of their thermo- and photo-stability, so the structure of the immobilized protein can be preserved even at temperatures above the denaturation temperature under normal conditions 28–30 . Such a strategy of immobilization of protein molecules could be very promising in the development of hybrid systems with increased stability and shelf-life for the next generation of nanostructured drugs.…”

Section: Introductionmentioning

confidence: 99%

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Sol-gel derived boehmite nanostructures is a versatile nanoplatform for biomedical applications

Solovev

Prilepskii

Krivoshapkina

et al. 2019

Sci Rep

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Alumina is one of the most promising carriers for drug delivery due to the long history of its usage as a vaccine adjuvant. Sol-gel synthesis provides excellent conditions for entrapment of biomolecules within an inorganic cage providing stabilization of proteins under the extremal conditions. In this paper, we show in vitro investigation of monodisperse alumina xerogel nanocontainers (AXNCs) using bovine serum albumin as a model protein entrapped in sol-gel alumina building blocks. Particularly, dose and cell-type dependent cytotoxicity in HeLa and A549 cancer cell lines were employed as well as investigation of antibacterial effect and stability of AXNCs in different biological media. It was shown, that the release of entrapped protein could be provided only in low pH buffer (as in cancer cell cytoplasm). This property could be applied for anticancer drug development. We also discovered boehmite nanoparticles effect on horizontal gene transfer and observed the appearance of antibiotic resistance by means of exchanging of the corresponding plasmid between two different E. coli strains. The present work may help to understand better the influence of AXNCs on various biological systems, such as prokaryotic and eukaryotic cells, and the activity of AXNCs in different biological media.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sol-gel derived boehmite nanostructures is a versatile nanoplatform for biomedical applications

Solovev

Prilepskii

Krivoshapkina

et al. 2019

Sci Rep

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“…The advantage of such materials was demonstrated in numerous studies, where silica sol-gel systems not only preserved the activity of the bioactive dopants but also provided exceptional chemical and thermal stability to a variety of proteins, enzymes, and even whole cells against various deterioration processes [ 39 , 40 , 41 , 42 ]. The attention towards silica materials is mainly dictated by their relative cheapness, convenience, and simple synthetic conditions, but alternative materials such as alumina, titania, or iron oxide-based systems can also be used for this purposes [ 43 , 44 , 45 , 46 ]. In our work, we used the original approach toward entrapment of biomolecules within sol-gel magnetite matrix at mild conditions based on readily available water-based magnetite hydrosol.…”

Section: Discussionmentioning

confidence: 99%

Structural Rearrangements of Carbonic Anhydrase Entrapped in Sol-Gel Magnetite Determined by ATR–FTIR Spectroscopy

Ivanovski

Shapovalova

Drozdov

2022

IJMS

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Enzymatically active nanocomposites are a perspective class of bioactive materials that finds their application in numerous fields of science and technology ranging from biosensors and therapeutic agents to industrial catalysts. Key properties of such systems are their stability and activity under various conditions, the problems that are addressed in any research devoted to this class of materials. Understanding the principles that govern these properties is critical to the development of the field, especially when it comes to a new class of bioactive systems. Recently, a new class of enzymatically doped magnetite-based sol-gel systems emerged and paved the way for a variety of potent bioactive magnetic materials with improved thermal stability. Such systems already showed themself as perspective industrial and therapeutic agents, but are still under intense investigation and many aspects are still unclear. Here we made a first attempt to describe the interaction of biomolecules with magnetite-based sol-gel materials and to investigate facets of protein structure rearrangements occurring within the pores of magnetite sol-gel matrix using ATR Fourier-transform infrared spectroscopy.

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“…125 Alumina gel also protected carbonic anhydrase, acid phosphatase and Horseradish peroxidase from photogedradation. 126 Alumina gels of bovine serum albumin are used as a protein carrier for biomedical applications. 127 Sol-gel methods for enzyme entrapment have been demonstrated to form a wide range of active biocatalytic materials, particularly for hydrophobic enzymes such as lipase.…”

Section: Sol-gel Methodsmentioning

confidence: 99%