Structural Rearrangements of Carbonic Anhydrase Entrapped in Sol-Gel Magnetite Determined by ATR–FTIR Spectroscopy

Ivanovski, Vladimir; Shapovalova, Olga E.; Drozdov, Andrey S.

doi:10.3390/ijms23115975

Cited by 7 publications

(3 citation statements)

References 53 publications

(69 reference statements)

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“…All MNPs are characterized by strong bands near 550 cm −1 with a shoulder at 630 cm −1 that correspond to Fe-O vibration and a broad band at 3000-3300 cm −1 due to -OH groups from adsorbed water molecules and Fe-OH groups. [46]. For spectra of hybrid materials, the broad band at 3500-3300 cm −1 is much more pronounced due to characteristic stretching vibrations of -OH and -N-H groups.…”

Section: Biomimetic Coating Of the Nanoparticlesmentioning

confidence: 98%

Bio-Inspired Surface Modification of Magnetite Nanoparticles with Dopamine Conjugates

et al. 2022

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Organically-coated nanomaterials are intensively studied and find numerous applications in a wide range of areas from optics to biomedicine. One of the recent trends in material science is the application of bio-mimetic polydopamine coatings that can be produced on a variety of substrates in a cost-efficient way under mild conditions. Such coatings not only modify the biocompatibility of the material but also add functional amino groups to the surface that can be further modified by classic conjugation techniques. Here we show an alternative strategy for substrates modification using dopamine conjugates instead of native dopamine. Compared to the classic scheme, the proposed strategy allows separation of the “organic” and “colloidal” stages, and simplified identification and purification steps. Modification with pre-modified dopamine made it possible to achieve high loading capacities with active components up to 10.5% wt. A series of organo-inorganic hybrids were synthesized and their bioactivity was analyzed.

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Section: Biomimetic Coating Of the Nanoparticlesmentioning

confidence: 98%

Bio-Inspired Surface Modification of Magnetite Nanoparticles with Dopamine Conjugates

et al. 2022

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“…The torque value was consistently higher than 10%. The viscosity of gels was determined by taking an average of three readings in a minute [17,18].…”

Section: Measurement Of Viscosity Of Mhg-flczmentioning

confidence: 99%

Design, Fabrication, in Vitro, and Ex-Vivo Permeation Study of Micro-Emulsified Hydrogel of Fluconazole (Mhg-Flcz) Using a Central Composite Design (Ccd)

GHOSH,

BASAK,

GANGULY

et al. 2024

Int J App Pharm

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Objective: The current study's objective was to develop and characterize a micro-hydrogel-based fluconazole (FLCZ) gel. A micro-hydrogel (Mhg) was prepared using different concentrations of Carbopol 940 (CP) and NaCMC using the modified swelling hydrogel method. Methods: A Preformulation study was performed using FTIR to confirm the drug and polymers were compatible with each other based on the functional group determination. 32 optimization procedures were used to develop formulations based on the response surface methodology. The prepared formulations were evaluated for entrapment efficiency, spreadability, viscosity, and visual examination using binocular microscopy and in vitro drug release using Franz diffusion cells. Results: The optimized formulation F2 reported entrapment efficiency of 65.09±0.41%, and viscosity of 11100±1.21 cps. The in vitro release of drug for the prepared formulations was performed for 8 h. and the optimized formulation showed better-controlled drug release compared to other formulations. It was observed that the optimized batch, percentage of drug permeability through the skin at 8 h of ex-vivo study shows 84.67±0.67% and in vitro drug release study (93.22%) through Franz diffusion cell, which suggests that the drug (Optimum batch) can easily penetrate through the skin and showed the highest drug release in a stipulated time interval. Conclusion: The use of an optimized Mhg-FLCZ gel formulation as it has excellent homogeneity, a pH that is close to that of the skin, and suitable thixotropic characteristics relates to that much more convenience than the conventional dosage form. The in vitro and ex-vivo study data proved its suitability as a better alternative to conventional products in the effective treatment of skin infections.

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“…The proteins have free movement at a local level, but the motion is highly restricted to the confined hollows, and most of the molecules are isolated and interact only with the matrix. Drozdov’s group immobilized CA into the pores of different sol–gel magnetite with singular magnetic properties using this method [ 147 ]. They studied the physical properties of the new material as well as the overall structure of the enzyme, mainly using infrared spectroscopy, concluding that the protein maintains its 3D arrangement in the generated nanoparticles.…”

Section: Improving Ca Performance: Enzyme Immobilizationmentioning

confidence: 99%

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

et al. 2023

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Direct biocatalytic processes for CO2 capture and transformation in value-added chemicals may be considered a useful tool for reducing the concentration of this greenhouse gas in the atmosphere. Among the other enzymes, carbonic anhydrase (CA) and formate dehydrogenase (FDH) are two key biocatalysts suitable for this challenge, facilitating the uptake of carbon dioxide from the atmosphere in complementary ways. Carbonic anhydrases accelerate CO2 uptake by promoting its solubility in water in the form of hydrogen carbonate as the first step in converting the gas into a species widely used in carbon capture storage and its utilization processes (CCSU), particularly in carbonation and mineralization methods. On the other hand, formate dehydrogenases represent the biocatalytic machinery evolved by certain organisms to convert CO2 into enriched, reduced, and easily transportable hydrogen species, such as formic acid, via enzymatic cascade systems that obtain energy from chemical species, electrochemical sources, or light. Formic acid is the basis for fixing C1-carbon species to other, more reduced molecules. In this review, the state-of-the-art of both methods of CO2 uptake is assessed, highlighting the biotechnological approaches that have been developed using both enzymes.

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Structural Rearrangements of Carbonic Anhydrase Entrapped in Sol-Gel Magnetite Determined by ATR–FTIR Spectroscopy

Cited by 7 publications

References 53 publications

Bio-Inspired Surface Modification of Magnetite Nanoparticles with Dopamine Conjugates

Bio-Inspired Surface Modification of Magnetite Nanoparticles with Dopamine Conjugates

Design, Fabrication, in Vitro, and Ex-Vivo Permeation Study of Micro-Emulsified Hydrogel of Fluconazole (Mhg-Flcz) Using a Central Composite Design (Ccd)

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

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