Protection from spinal cord ischemia-reperfusion damage with  alpha-lipoic acid preconditioning in an animal model

Kumbasar, Ulaş; Demirci, Harun; Emmez, Gökçen; Yıldırım, Zühal; Gönül, İpek Işık; Emmez, Hakan; Kaymaz, Memduh

doi:10.5606/tgkdc.dergisi.2018.14432

Cited by 7 publications

(4 citation statements)

References 44 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The GSH protects cells against oxidative damage due to the reaction to free radicals and peroxides. Kumbasar et al [25] reported that they detected the GSH level in their study using ALA. In our study, blood GSH values were found to be significantly higher in the ALA group compared to the other groups.…”

Section: Discussionmentioning

confidence: 96%

The comparison of alpha lipoic acid with methylprednisolone and sucralfate in subacute wound healing corrosive esophagus-induced rats: An experimental study

Gültekin¹,

Ceran

Gültekin

2023

Turk Gogus Kalp Dama

View full text Add to dashboard Cite

Background: This study aims to compare methylprednisolone frequently used in the therapeutic practices of corrosive esophagus burns, sucralfate, a protective material of mucosal surfaces, and alpha lipoic acid, the most potent antioxidant in a rat model. Methods: A total of 40 female Sprague-Dawley rats were used in this study. The rats were equally divided into control, alpha lipoic acid, methylprednisolone, and sucralfate groups (n=10). A corrosive esophagus burn was created by using 10% pH:12 sodium hydroxide. No treatment was applied to the control group, and each group was given their own treatment. The treatment was continued regularly until the eighth day, when they were sacrificed. The corrosive esophagus burn lines were removed and tissue sections were stained with hematoxylin and eosin. Results: The difference in ulceration in the group treated with alpha lipoic acid was significant, compared to the other groups. The most excellent complete epithelialization and complete re-epithelialization were observed in the alpha lipoic acid group. The difference between the groups was significant, with complete re-epithelialization being the lowest in the control and methylprednisolone groups (42.9% and 12.5%, respectively) and the highest in the alpha lipoic acid group (77.8%). In terms of ulceration and re-epithelialization, comparable values were found in the alpha lipoic acid group. The main difference was that the inflammation levels in the sucralfate group were lower and more favorable than the other groups in this period. The glutathione level was significantly higher in the alpha lipoic acid group and decreased the tissue hydroxyproline level. Conclusion: Alpha lipoic acid reduces esophageal ulceration, severity and prevalence of inflammation, severity and prevalence of fibrosis, decreases tissue damage by increasing blood glutathione level, and also reduces stricture in corrosive esophagus burns in rats.

show abstract

Section: Discussionmentioning

confidence: 96%

The comparison of alpha lipoic acid with methylprednisolone and sucralfate in subacute wound healing corrosive esophagus-induced rats: An experimental study

Gültekin¹,

Ceran

Gültekin

2023

Turk Gogus Kalp Dama

View full text Add to dashboard Cite

show abstract

“…It provides reliable information about the every of the peroxidation reaction and tissue damage, and is used as a marker in determining oxidative damage. [ 4 ] The I /Rep has been reported to cause a significant increase in MDA levels in lung tissues in studies involving clamping of the IAA and in studies involving pulmonary hilus clamping. [ 4 , 14 , 16 , 17 , 20 ] Similarly, in the present study, and consistent with previous research, we observed that I/Rep increased the lung tissue MDA levels.…”

Section: Discussionmentioning

confidence: 99%

“…[ 15 , 16 ] Yaman et al[ 16 ] reported depletion of endogenous GSH due to I/Rep and low GSH levels in lung tissue in I/Rep group. Kumbasar et al[ 4 ] reported significant decreases in both GSH and CAT levels in lung tissue after I/Rep. On the other hand, Cevirme et al[ 15 ] did not observe that I/Rep damage caused by IAA occlusion caused a decrease in GSH levels in the lung tissue.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol against lung injury in an ischemia/reperfusion model of abdominal aortic rupture

Karakişi¹,

Hemsinli²,

Tümkaya³

et al. 2021

Turk Gogus Kalp Dama

View full text Add to dashboard Cite

Background: The aim of this study was to examine the effects on the lungs of ischemia/reperfusion injury in a ruptured abdominal aortic aneurysm model in rats and to investigate the potential protective effects of resveratrol. Methods: Thirty-two male Sprague-Dawley rats were randomly divided into four groups: control, ischemia/reperfusion, sham (ischemia/ reperfusion + solvent/dimethyl sulfoxide), and ischemia/reperfusion + resveratrol. In the groups subjected to ischemia/reperfusion, following 60-min shock to the abdominal aorta, vascular clamps were attached from the levels of the infrarenal and iliac bifurcation. A total of 60-min ischemia was applied, followed by 120-min reperfusion. In the ischemia/ reperfusion + resveratrol group, intraperitoneal 10 mg/kg resveratrol was administered 15 min before ischemia and immediately after reperfusion. Malondialdehyde, glutathione, and catalase levels were analyzed and histopathological examination of the lung tissues was performed. Results: Malondialdehyde levels increased in the ischemia/reperfusion and ischemia/reperfusion + dimethyl sulfoxide groups, compared to the control group, while catalase levels decreased, and no significant difference was observed in the glutathione levels. Malondialdehyde levels decreased with the administration of resveratrol, while glutathione levels increased, and catalase levels remained unchanged. The increased inflammation in interstitial spaces, thickening in the alveolar septal walls, increased numbers of cleaved caspase-3 apoptotic pneumocytes, and increased histopathological lung damage scores observed in the ischemia/reperfusion and ischemia/reperfusion + dimethyl sulfoxide groups improved with the application of resveratrol. Conclusion: These findings suggest that resveratrol may exhibit a protective effect in preventing acute lung injury developing due to ischemia/reperfusion in ruptured abdominal aortic aneurysm surgery by reducing oxidative damage.

show abstract

“…MDA levels in the serum and femur of SCI rats in the exogenous H 2 S treatment group were also reduced, suggesting that exogenous H 2 S treatment could attenuate the oxidative stress caused by SCI. In rabbits with ischemic SCI, α‐lipoic acid administration before aortic blockade was able to reduce plasma and spinal cord levels of

{NO}^{\cdot -}

, GSH, MDA, and late oxidized protein products; it had a significant neuroprotective effect (Kumbasar et al, 2018).…”

Section: Treatment Of Sci Based On Drugs With Antioxidative Effectsmentioning

confidence: 99%

Oxidative stress following spinal cord injury: From molecular mechanisms to therapeutic targets

Yu,

Wang,

Wang

et al. 2023

J of Neuroscience Research

View full text Add to dashboard Cite

Spinal cord injury (SCI) is a medical condition that results from severe trauma to the central nervous system; it imposes great psychological and economic burdens on affected patients and their families. The dynamic balance between reactive oxygen species (ROS) and antioxidants is essential for maintaining normal cellular physiological functions. As important intracellular signaling molecules, ROS regulate numerous physiological activities, including vascular reactivity and neuronal function. However, excessive ROS can cause damage to cellular macromolecules, including DNA, lipids, and proteins; this damage eventually leads to cell death. This review discusses the mechanisms of oxidative stress in SCI and describes some signaling pathways that regulate oxidative injury after injury, with the aim of providing guidance for the development of novel SCI treatment strategies.

show abstract

Protection from spinal cord ischemia-reperfusion damage with alpha-lipoic acid preconditioning in an animal model

Cited by 7 publications

References 44 publications

The comparison of alpha lipoic acid with methylprednisolone and sucralfate in subacute wound healing corrosive esophagus-induced rats: An experimental study

The comparison of alpha lipoic acid with methylprednisolone and sucralfate in subacute wound healing corrosive esophagus-induced rats: An experimental study

Resveratrol against lung injury in an ischemia/reperfusion model of abdominal aortic rupture

Oxidative stress following spinal cord injury: From molecular mechanisms to therapeutic targets

Contact Info

Product

Resources

About