Resveratrol against lung injury in an ischemia/reperfusion model of abdominal aortic rupture

Abstract

Background: The aim of this study was to examine the effects on the lungs of ischemia/reperfusion injury in a ruptured abdominal aortic aneurysm model in rats and to investigate the potential protective effects of resveratrol. Methods: Thirty-two male Sprague-Dawley rats were randomly divided into four groups: control, ischemia/reperfusion, sham (ischemia/ reperfusion + solvent/dimethyl sulfoxide), and ischemia/reperfusion + resveratrol. In the groups subjected to ischemia/reperfusion, following 60-min shock to the abdominal aorta, vascular clamps were attached from the levels of the infrarenal and iliac bifurcation. A total of 60-min ischemia was applied, followed by 120-min reperfusion. In the ischemia/ reperfusion + resveratrol group, intraperitoneal 10 mg/kg resveratrol was administered 15 min before ischemia and immediately after reperfusion. Malondialdehyde, glutathione, and catalase levels were analyzed and histopathological examination of the lung tissues was performed. Results: Malondialdehyde levels increased in the ischemia/reperfusion and ischemia/reperfusion + dimethyl sulfoxide groups, compared to the control group, while catalase levels decreased, and no significant difference was observed in the glutathione levels. Malondialdehyde levels decreased with the administration of resveratrol, while glutathione levels increased, and catalase levels remained unchanged. The increased inflammation in interstitial spaces, thickening in the alveolar septal walls, increased numbers of cleaved caspase-3 apoptotic pneumocytes, and increased histopathological lung damage scores observed in the ischemia/reperfusion and ischemia/reperfusion + dimethyl sulfoxide groups improved with the application of resveratrol. Conclusion: These findings suggest that resveratrol may exhibit a protective effect in preventing acute lung injury developing due to ischemia/reperfusion in ruptured abdominal aortic aneurysm surgery by reducing oxidative damage.

Protective effect of clotrimazole on lung injury in an experimental model of ruptured abdominal aortic aneurysm

“…7 Total body hypoperfusion due to hemorrhagic shock or lower body ischemia due to aortic clamping alone does not cause sufficient organ damage and increased mortality. 6,[23][24][25] Due to these 2 crucial ischemic conditions and subsequent reperfusion in RAAA operations, mortality remains high, and multiple organ failures occur. 5,7 Although many physiopathological factors that cause shock and I/R injury are considered, the main factor is free oxygen radicals that appear in the reperfusion phase.…”

Hypoxic Conditioning: A Potential Perioperative Strategy to Reduce Abdominal Aortic Occlusion-Related Injury in Mouse Proximal and Distal Organs

“…Abdominal aortic occlusion (AAO), also referred to as aortic clamping, is a surgical procedure that involves temporary or permanent occlusion of the abdominal aorta [1]. This procedure has various clinical applications such as controlling bleeding from the abdominal organs, abdominal aortic aneurysm repair, organ transplantation and providing a bloodless field for surgical procedures [2].…”

Abdominal Aortic Occlusion and the Inflammatory Effects in Heart and Brain

“…There are several clinical circumstances that can lead to abdominal aortic occlusion (AAO)-induced ischemia/reperfusion (I/R) injury, such as perivascular surgery, repair of abdominal aortic aneurysms or dissections, acute abdominal aortic thromboembolism, emergency trauma surgery, or balloon occlusion therapy after abdominal blood loss [ 1 , 2 ]. Even though such clinical phenomena are known to cause ischemic and ischemia/reperfusion injuries in organs directly affected by reduced blood supply from the aorta (kidneys, gastrointestinal tract, lower limb muscles), other remote organs may also be affected, such as the heart and lung [ 3 , 4 ].…”