Protease Production by Different Thermophilic Fungi

Abstract

A comparative study was carried out to evaluate protease production in solid-state fermentation (SSF) and submerged fermentation (SmF) by nine different thermophilic fungi--Thermoascus aurantiacus Miehe, Thermomyces lanuginosus, T. lanuginosus TO.03, Aspergillus flavus 1.2, Aspergillus sp. 13.33, Aspergillus sp. 13.34, Aspergillus sp. 13.35, Rhizomucor pusillus 13.36 and Rhizomucor sp. 13.37--using substrates containing proteins to induce enzyme secretion. Soybean extract (soybean milk), soybean flour, milk powder, rice, and wheat bran were tested. The most satisfactory results were obtained when using wheat bran in SSF. The fungi that stood out in SSF were T. lanuginosus, T. lanuginosus TO.03, Aspergillus sp. 13.34, Aspergillus sp. 13.35, and Rhizomucor sp. 13.37, and those in SmF were T. aurantiacus, T. lanuginosus TO.03, and 13.37. In both fermentation systems, A. flavus 1.2 and R. pusillus 13.36 presented the lowest levels of proteolytic activity.

Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation

“…The first production obtained a peak around 370 U/mL after 96 h of fermentation and the optimization led to an increase of 1.8 times of activity and shifted the peak for 72 h of the bioprocess. Different species of Aspergillus sp in similar culture medium showed peptidase production similar to the production in this work, with peaks of 400 to 800 U/mL, as reported by Macchione et al (2008). In a study using the same culture medium (5 g of WB) with supplementation with egg albumin and casein, the production of peptidase by Penicillium corylophilum reached a peak of 520 U/mL after 96 h using only WB, and the production by Penicillium waksmanii was slightly increased using supplementation, with peak of 545 U/mL after 72 h. However, the supplementation could be removed to generate cheaper medium because the peak with 5 g of WB alone was approximately 500 U/mL (Silva et al 2013a).…”

Production of thermostable xylanase by thermophilic fungal strains isolated from maize silage

“…Some studies report that Rh. pusillus (Macchione, Merheb, Gomes, & Da Silva, 2008;Yegin, Fernández-Lahore, Guvenc, & Goksungur, 2010) and A. fumigatus (Neustadt et al, 2009;Oguma et al, 2011) are protease producer strains.…”

Bioprocess development to add value to canola cake used as substrate for proteolytic enzyme production

“…For example, Macchione et al (2008) and Sandhya et al (2005) carried out comparative studies using different fungal strains to evaluate their performance in protease production. Thanapimmetha et al (2012) investigated the production of protease by Aspergillus oryzae cultivated under SSF using deoiled Jatropha curcas seed cake as substrate.…”