Protamine-coated silica gel as packing material for high-performance liquid chromatography of carbohydrates

Yamauchi, Shuji; Nimura, Noriyuki; Kinoshita, Toshio

doi:10.1039/an9931800161

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…New stationary phases for the analysis of carbohydrates such as cyclodextrin bonded phase columns [28], protamine-coated silica gel packing material [29] and graphitized carbon columns [30] have been developed recently. Refractive index detection (RI) and UV detection in the range of 190 to 210 nm are mainly used in routine carbohydrate analysis.…”

Section: Introductionmentioning

confidence: 99%

HPLC analysis of carbohydrates on POLYSPHER®CH OH columns using pulsed amperometric detection (PAD) with sodium hydroxide as post column detection reagent

Gey

Unger

Battermann

1996

Fresenius J Anal Chem

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Carbohydrates have been separated on POLYSPHER(R)CH OH columns using pulsed amperometric detection (PAD) and UV detection (lambda =196 nm) in series and pure water as mobile phase. Nearly baseline separations have been obtained for the glycoprotein carbohydrates of sialic acid ( N-acetylneuraminic acid, NANA), N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc). As carbohydrates dissolved and eluted with pure water are present in the neutral form they are not detectable with PAD in contrast to carbohydrate anions formed at high pH values. Therefore an additional NaOH post column reagent has been continuously pumped through a mixing chamber into the mobile phase to form carbohydrate anions resulting in improved detection limits. Monosaccharides as well as glycoprotein carbohydrates could be detected in the microg/ml-range. This method has been applied successfully to the analysis of sugars in fruit juice. With only 2 microl of juice per 50 ml water, the determination of the main constituents, sucrose, glucose and fructose, was possible in a few minutes without sample preparation.

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Section: Introductionmentioning

confidence: 99%