Development of protamine-bonded phase for separation of saccharides in liquid chromatography

Biologically active compounds such as vitamins, steroids, nucleosides, peptides and proteins play a very important role in coordinating living organism functions. Determination of those substances is indispensable in pathogenesis. Their complex structure and physico-chemical properties cause many analytical problems. Chromatography is the most common technique used in pharmaceutical and biomedical analysis. The interaction between analyte and stationary phase plays a major role in the separation process. The structure of the packing has a significant influence on the results of the separation process. Various types of spectroscopic techniques, such as nuclear magnetic resonance spectroscopy, infrared spectroscopy, fluorescence spectroscopy and photoacoustic spectroscopy can be useful tools for the characterization of packings. Surface area measurements, elemental analysis, thermal analysis and microcalorimetric measurements are also helpful in this field. Part of the paper contains a description of chromatographic tests used for the determination of column properties. The description of the possibilities of surface characterization is not complete, but is focused on the most popular techniques and practical chromatographic tests. All the presented methods made possible the design and quality control of a new generation stationary phases, which are the future of high-performance liquid chromatography.

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Some remarks on characterization and application of stationary phases for RP-HPLC determination of biologically important compounds

Kowalska

Krupczyńska

Buszewski

2005

Biomed. Chromatogr.

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Column Liquid Chromatography: Equipment and Instrumentation

LaCourse

Dasenbrock

1998

Anal. Chem.

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Computational Chemical Analysis of Newly Developed Guanidino-Phase for Quantitative Analysis of Saccharides in Liquid Chromatography

Hanai¹,

Miyazaki

Suzuki

et al. 1997

Journal of Liquid Chromatography & Related Technologies

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Development of protamine-bonded phase for separation of saccharides in liquid chromatography

Cited by 3 publications

References 20 publications

Some remarks on characterization and application of stationary phases for RP-HPLC determination of biologically important compounds

Some remarks on characterization and application of stationary phases for RP-HPLC determination of biologically important compounds

Column Liquid Chromatography: Equipment and Instrumentation

Computational Chemical Analysis of Newly Developed Guanidino-Phase for Quantitative Analysis of Saccharides in Liquid Chromatography

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