2002
DOI: 10.1016/s0301-4770(02)80040-9
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Chapter 15 Preparative HPLC of carbohydrates

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“…Depending on the application, a trade-off should be considered between purity and product yield, decreasing reaction time if the former is favored and increasing the reaction time if the latter is preferred. Alternatively, either preparative high-performance liquid chromatography (HPLC) [ 36 , 37 ] or simulated moving bed chromatography might be used to separate monosaccharides, as established by the production of psicose from sucrose with a three-enzyme cascade reaction [ 9 ]. A more elegant solution might also be provided by adding an additional enzyme to the reaction mixture, creating a coupled process.…”
Section: Discussionmentioning
confidence: 99%
“…Depending on the application, a trade-off should be considered between purity and product yield, decreasing reaction time if the former is favored and increasing the reaction time if the latter is preferred. Alternatively, either preparative high-performance liquid chromatography (HPLC) [ 36 , 37 ] or simulated moving bed chromatography might be used to separate monosaccharides, as established by the production of psicose from sucrose with a three-enzyme cascade reaction [ 9 ]. A more elegant solution might also be provided by adding an additional enzyme to the reaction mixture, creating a coupled process.…”
Section: Discussionmentioning
confidence: 99%