Proposing a high-efficiency dielectrophoretic system for separation of dead and live cells

Shayestehpour, H.; Nazif, Koosha Nassiri; Soufi, A. M.; Saidi, Maysam

doi:10.24200/sci.2017.4502

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…Particle trajectories were simulated numerically to obtain the particle behavior. More detailed discussion regarding the numerical simulation of the same scheme was reported in our previous studies. , Finally, experimental results are presented for each category, and the effects of the experimentally controllable parameters (applied voltage amplitude and inlet flow rate) and particle size on performance are studied.…”

Section: Resultsmentioning

confidence: 99%

“…Comprehensive numerical modeling was carried out previously by our group to optimize DEP-based particle separation. , The numerical simulation indicated highly efficient separation of dead and live cells. In order to model the function of the proposed dielectrophoretic device, flow field and electric field were first solved.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Comprehensive numerical modeling was carried out previously by our group to optimize DEPbased particle separation. 49,50 The numerical simulation…”

Section: Numerical Simulationsmentioning

confidence: 99%

See 2 more Smart Citations

Simple, Cost-Effective, and Continuous 3D Dielectrophoretic Microchip for Concentration and Separation of Bioparticles

Tajik

Saidi

Kashaninejad

et al. 2019

Ind. Eng. Chem. Res.

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Dielectrophoresis is a robust approach for manipulating bioparticles in microfluidic devices. In recent years, many groups have developed dielectrophoresis-based microfluidic systems for separation and concentration of various types of bioparticles, where the gradient of the electric field causes dielectrophoresis force acting on the suspended particles. Enhancing the gradient of the electric field with three-dimensional (3D) electrodes can significantly improve the efficiency of the system. Implementing planar electrodes in a 3D arrangement is a simple option to form a 3D-electrode configuration. This paper reports the development of a novel dielectrophoretic microfluidic system for continuously manipulating microparticles such as polystyrene microbeads and Saccharomyces cerevisiae cells. The fabrication process was relatively simple, cost-effective, and precise. Moreover, the device was tested to find the impact of various parameters on the concentration of polystyrene microbeads and separation of live and dead cells. The optimum working conditions, including flow rate, applied voltage amplitude, and frequency, were obtained accordingly. Furthermore, the experimentally observed trajectories of the particles agreed well with simulated counterparts. The device was able to efficiently and continuously perform with high throughput. Under an optimum condition, an efficiency of approximately 100% was obtained, confirming the capability of the proposed design with four triangular electrodes for continuous focusing and separation of live and dead cells as well as polystyrene particles.

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Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Simple, Cost-Effective, and Continuous 3D Dielectrophoretic Microchip for Concentration and Separation of Bioparticles

Tajik

Saidi

Kashaninejad

et al. 2019

Ind. Eng. Chem. Res.

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“…DEP has been proven as an adaptable mechanism to transportation, accumulation, separation and characterization of submicron bio-particles in microfluidic systems. The integration of DEP systems into the microfluidics provides the inexpensive, fast, highly sensitive, selective and label-free detection of bio-species [15][16][17][18][19][20][21][22][23].…”

Section: Dielectrophoresismentioning

confidence: 99%

An Improved Escherichia Coli Bacterium Detection in Microchannel Based on Dielectrophoresis Impedance Measurements

2020

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Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications

et al. 2022

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Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein. Extensive studies have been carried out on protein DEP, especially on Bovine Serum Albumin (BSA). However, these studies involve the usage of dye and fluorescent probes to observe DEP responses as the physical properties of protein albumin molecular structure are translucent. The use of dye and the fluorescent probe could later affect the protein’s physiology. In this article, we review three methods of electrical quantification of DEP responses: electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and capacitance measurement for protein BSA DEP manipulation. The correlation of these methods with DEP responses is further discussed. Based on the observations on capacitance measurement, it can be deduced that the electrical quantifying method is reliable for identifying DEP responses. Further, the possibility of manipulating the protein and electrically quantifying DEP responses while retaining the original physiology of the protein and without the usage of dye or fluorescent probe is discussed.

show abstract

Proposing a high-efficiency dielectrophoretic system for separation of dead and live cells

Cited by 3 publications

References 24 publications

Simple, Cost-Effective, and Continuous 3D Dielectrophoretic Microchip for Concentration and Separation of Bioparticles

Simple, Cost-Effective, and Continuous 3D Dielectrophoretic Microchip for Concentration and Separation of Bioparticles

An Improved Escherichia Coli Bacterium Detection in Microchannel Based on Dielectrophoresis Impedance Measurements

Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications

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