Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications

Nasir, Nur Shahira Abdul; Deivasigamani, Revathy; Wee, M. F. Mohd Razip; Hamzah, Azrul Azlan; Zaid, Mohd Hazani Mat; Rahim, Muhammad Khairulanwar Abdul; Kayani, Aminuddin Bin Ahmad; Abdulhameed, Abdullah; Buyong, Muhamad Ramdzan

doi:10.3390/mi13081308

Cited by 4 publications

(6 citation statements)

References 104 publications

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“…The Clausius-Mossotti factor (CMF) is related to the effective polarizability of the particle, E is the root-mean-square value of the applied electric field given by Equation ( 2). 33 The CMF is a unitless number that quantifies the interaction between the particle and medium in terms of their respective complex polarizabilities and determines the F DEP direction. It is written below in its complex form,…”

Section: Dep Theorymentioning

confidence: 99%

“…

F_{italicDEP} = 2 π r^{3} ε_{0} ε_{m} Re [italicCMF] \nabla E^{2}

where ε m is the absolute permittivity of the surrounding medium, r is the particle radius. The Clausius‐Mossotti factor (CMF) is related to the effective polarizability of the particle, E is the root‐mean‐square value of the applied electric field given by Equation () 33 . The CMF is a unitless number that quantifies the interaction between the particle and medium in terms of their respective complex polarizabilities and determines the F DEP direction.…”

Section: Dep Theorymentioning

confidence: 99%

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A dielectrophoresis proof of concept of polystyrene particles and in‐vitro human epidermal keratinocytes migration for wound rejuvenation

Deivasigamani

Maidin

Nasir

et al. 2022

J of Applied Polymer Sci

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Diabetes affects approximately 170 million people worldwide, is expected to double by 2030, and is a severe problem. Electrical stimulation (ES) via dielectrophoresis (DEP) technique may be an effective alternative in enhancing healing rates in diabetic patients with open ulcers. This research used DEP force (FDEP) to manipulate 3.2, 4.8, 10, and 15 μm polystyrene (PS) particles to predict the migration capability of human epidermal keratinocytes (HEKs). A numerical modeling method, MyDEP, was used to predict the interpretation of Clausius–Mossotti factors of PS particles and HEKs. The finite element method computes the electric field intensity and particle trajectory based on DEP and drag forces in their respective medium. DEP experiments on numerous size PS particles and alive HEKs were carried out in a tapered aluminium microelectrode array using a non‐uniform electric field. The distinct PS particles exhibit positive DEP (PDEP), crossover frequency (fXO), and negative DEP (NDEP), whereas the HEKs experience, only NDEP due to its high conductive medium in frequency ranges from 100 kHz to 1 MHz. Finally, the DIPP‐MotionV analysis shows that particle mobility between speed and acceleration is statistically considerable. When an appropriate frequency is applied to HEKs in random locations, the FDEP aligns at the desired target position based on its dielectric properties, which accelerates wound healing in in‐vivo conditions.

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Section: Dep Theorymentioning

confidence: 99%

“…

F_{italicDEP} = 2 π r^{3} ε_{0} ε_{m} Re [italicCMF] \nabla E^{2}

Section: Dep Theorymentioning

confidence: 99%

A dielectrophoresis proof of concept of polystyrene particles and in‐vitro human epidermal keratinocytes migration for wound rejuvenation

Deivasigamani

Maidin

Nasir

et al. 2022

J of Applied Polymer Sci

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“…Characterization of Staphylococcus aureus using DEP for DEP response pDEP, crossover frequency and nDEP in this recent years is important for detection, manipulation and isolation of the cells [3,4]. Based on past research, DEP devices focusing on 80 µm interelectrode gap for biological application [5][6][7][8][9]. In this work, we improvised DEP technique by decreasing interelectrode gap device to 20 µm for characterization of Staphylococcus aureus.…”

Section: Introductionmentioning

confidence: 99%

Characterization of 20 μm Dielectrophoretic Interelectrode Gap for Staphylococcus Aureus Rapid Detection Application

Arash Zulkarnain Ahmad Rozaini,

Abdullah Abdulhameed,

Revathy Deivasigamani

et al. 2024

IJNeaM

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We propose an improvement of dielectrophoresis technique (DEP) by designing, simulating and experiment a 20 μm interelectrode gap for Staphylococcus aureus rapid detection application. In this paper, we use MyDEP simulation for DEP polarization Staphylococcus aureus on frequencies range. COMSOL simulation is utilized for comparison of 20 μm and 80 μm interelectrode gap based on trajectory and velocity of particles for Staphylococcus aureus application. We implied 20 μm interelectrode gap for Staphylococcus aureus application produced higher magnitude DEP force. Which gives accurate trajectory characterization and higher velocity of particle movement. DEP characterization using small interelectrode gap is capable of producing stable and optimum DEP value. It is demanding to distinguish the simulation Staphylococcus aureus using experimental method. DEP technique is important for analysis and characterization of the bacteria. The result show that 20 μm interelectrode gap has higher intensity of electric field which is 1.51 x 10^6 V/m in COMSOL simulation and produced 20.1 m/s for velocity of particle trajectories which is higher compared to 80 μm interelectrode gap. The DEP response was tested on 2.5 MHz, as crossover frequency response was observed on experimental and simulation. Thus, supportsthe capability of 20 μm interelectrode gap for Staphylococcus aureus rapid detection application. Furthermore, DEP characterization can be improvised by focusing on interelectrode gap for characterization among bacteria cells, critical for bacteria detection, manipulation, and isolation.

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“…Recently Nasir et al. [42] reported on the protein albumin manipulation and electrical quantification of molecular DEP in bio‐applications.…”

Section: Introductionmentioning

confidence: 99%

“…Turcan and Olariu [32] focused on the electromanipulation and electrical characterization of various cancerous cells. Recently Nasir et al [42] reported on the protein albumin manipulation and electrical quantification of molecular DEP in bio-applications.…”

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confidence: 99%

A correlation of conductivity medium and bioparticle viability on dielectrophoresis‐based biomedical applications

et al. 2023

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Dielectrophoresis (DEP) bioparticle research has progressed from micro to nano levels. It has proven to be a promising and powerful cell manipulation method with an accurate, quick, inexpensive, and label-free technique for therapeutic purposes. DEP, an electrokinetic phenomenon, induces particle movement as a result of polarization effects in a nonuniform electrical field. This review focuses on current research in the biomedical field that demonstrates a practical approach to DEP in terms of cell separation, trapping, discrimination, and enrichment under the influence of the conductive medium in correlation with bioparticle viability. The current review aims to provide readers with an in-depth knowledge of the fundamental theory and principles of the DEP technique, which is influenced by conductive medium and to identify and demonstrate the biomedical application areas. The high conductivity of physiological fluids presents obstacles and opportunities, followed by bioparticle viability in an electric field elaborated in detail. Finally, the drawbacks of DEP-based systems

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Protein Albumin Manipulation and Electrical Quantification of Molecular Dielectrophoresis Responses for Biomedical Applications

Cited by 4 publications

References 104 publications

A dielectrophoresis proof of concept of polystyrene particles and in‐vitro human epidermal keratinocytes migration for wound rejuvenation

A dielectrophoresis proof of concept of polystyrene particles and in‐vitro human epidermal keratinocytes migration for wound rejuvenation

Characterization of 20 μm Dielectrophoretic Interelectrode Gap for Staphylococcus Aureus Rapid Detection Application

A correlation of conductivity medium and bioparticle viability on dielectrophoresis‐based biomedical applications

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