Simple, Cost-Effective, and Continuous 3D Dielectrophoretic Microchip for Concentration and Separation of Bioparticles

Tajik, Parham; Saidi, Mohammad R.; Kashaninejad, Navid; Nguyen, Nam‐Trung

doi:10.1021/acs.iecr.9b00771

Cited by 37 publications

(27 citation statements)

References 59 publications

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“…Separation between blood cells and cancer cells was done at a high cell ratio of 1:10 000 using side‐wall electrodes, but the flow rate was 0.1 μL/min [41]. 3D electrodes were applied to generate DEP for the separation of particles or yeast cells [42]. The device needed precise vertical alignment and was operated at low concentration.…”

Section: Resultsmentioning

confidence: 99%

Study on the discrete dielectrophoresis for particle–cell separation

et al. 2020

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Study on the discrete dielectrophoresis for particle-cell separationThis paper presents the application of the discrete dielectrophoretic force to separate polystyrene particles from red blood cells. The separation process employs a simple microfluidic device that is composed of interdigitated electrodes and a microchannel. The discrete dielectrophoretic force is generated by adjusting the duty cycle of the applied voltage. The electrodes make a tilt angle with the microchannel to change the moving direction of the red blood cells. By adjusting the voltage magnitude and duty cycle, we investigate the deflection of red blood cells and the variation of cell velocity along electrode edge under positive dielectrophoresis. The experiments with polystyrene particles show that the enrichment of the particles is greater than 150 times. The maximum separation efficiency is 97% for particle-to-cell number ratio equal to 1:2000 in the sample having high cell concentration. Using the appropriate applied voltage magnitude and duty cycle, the discrete dielectrophoretic force can prevent the clogging of microchannel while successfully separating the particles from the cells with high enrichment and efficiency. The proposed principle can be readily applied to dielectrophoresis-based devices for biomedical sample preparation or diagnosis such as the separation of rare or infected cells from a blood sample.

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Section: Resultsmentioning

confidence: 99%

Study on the discrete dielectrophoresis for particle–cell separation

et al. 2020

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“…( E) eDEP device used to separate particles with different properties; in this case, live and dead yeast cells. Reprinted with permission from [17] copyright (2019) American Chemical Society. ( F) Contactless dielectrophoresis device used to sort three different particles based on their size.…”

Section: Introductionmentioning

confidence: 99%

Toward low‐voltage dielectrophoresis‐based microfluidic systems: A review

2020

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Dielectrophoretically driven microfluidic devices have demonstrated great applicability in biomedical engineering, diagnostic medicine, and biological research. One of the potential fields of application for this technology is in point‐of‐care (POC) devices, ideally allowing for portable, fully integrated, easy to use, low‐cost diagnostic platforms. Two main approaches exist to induce dielectrophoresis (DEP) on suspended particles, that is, electrode‐based DEP and insulator‐based DEP, each featuring different advantages and disadvantages. However, a shared concern lies in the input voltage used to generate the electric field necessary for DEP to take place. Therefore, input voltage can determine portability of a microfluidic device. This review outlines the recent advances in reducing stimulation voltage requirements in DEP‐driven microfluidics.

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“…The model was used for parametric study. Tajik et al [ 18 ] developed a microfluidic device with four right-triangle shaped electrodes; two electrodes are placed on the top surface while the other two electrodes are placed on the bottom surface of the microchannel. Each electrode is positioned with one edge in contact with one of the sidewalls and a second edge perpendicular to the same sidewall; additionally, the leading-edge width of the electrode is zero.…”

Section: Introductionmentioning

confidence: 99%

“…With this electrode configuration, type-based separation is achieved by subjecting one type of microparticle to pDEP, which is subsequently drawn to the region between the top and bottom electrodes, while the other type of microparticle is acted upon by nDEP to be pushed toward the center of the microchannel. Tajik et al [ 18 ] modeled the microfluidic device by including the influence of phenomena such as drag and DEP.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Microfluidic Device for Separating Microparticles Based on Size with Sub-Micron Resolution

et al. 2020

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This article details the mathematical model of a microfluidic device aimed at separating any binary heterogeneous sample of microparticles into two homogeneous samples based on size with sub-micron resolution. The device consists of two sections, where the upstream section is dedicated to focusing of microparticles, while the downstream section is dedicated to separation of the focused stream of microparticles into two samples based on size. Each section has multiple planar electrodes of finite size protruding into the microchannel from the top and bottom of each sidewall; each top electrode aligns with a bottom electrode and they form a pair leading to multiple pairs of electrodes on each side. The focusing section subjects all microparticles to repulsive dielectrophoretic force, from each set of the electrodes, to focus them next to one of the sidewalls. This separation section pushes the big microparticles toward the interior, away from the wall, of the microchannel using repulsive dielectrophoretic force, while the small microparticles move unaffected to achieve the desired degree of separation. The operating frequency of the set of electrodes in the separation section is maintained equal to the cross-over frequency of the small microparticles. The working of the device is demonstrated by separating a heterogeneous mixture consisting of polystyrene microparticles of different size (radii of 2 and 2.25 μm) into two homogeneous samples. The mathematical model is used for parametric study, and the performance is quantified in terms of separation efficiency and separation purity; the parameters considered include applied electric voltages, electrode dimensions, outlet widths, number of electrodes, and volumetric flowrate. The separation efficiencies and separation purities for both microparticles are 100% for low volumetric flow rates, a large number of electrode pairs, large electrode dimensions, and high differences between voltages in both sections.

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Simple, Cost-Effective, and Continuous 3D Dielectrophoretic Microchip for Concentration and Separation of Bioparticles

Cited by 37 publications

References 59 publications

Study on the discrete dielectrophoresis for particle–cell separation

Study on the discrete dielectrophoresis for particle–cell separation

Toward low‐voltage dielectrophoresis‐based microfluidic systems: A review

Dielectrophoretic Microfluidic Device for Separating Microparticles Based on Size with Sub-Micron Resolution

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