Proposal of Burkholderia gen. nov. and Transfer of Seven Species of the Genus Pseudomonas Homology Group II to the New Genus, with the Type Species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov.

Abstract

Based on the 16S rRNA sequences, DNA‐DNA homology values, cellular lipid and fatty acid composition, and phenotypic characteristics, a new genus Burkholderia is proposed for the RNA homology group II of genus Pseudomonas. Seven species in this group were transfered to the new genus. Thus seven new combinations, Burkholderia cepacia (Palleroni and Holmes 1981), Burkholderia mallei (Zopf 1885), Burkholderia pseudomallei (Whitmore 1913), Burkholderia caryophylli (Burkholder 1942), Burkholderia gladioli (Severini 1913), Burkholderia pickettii (Ralston et al 1973) and Burkholderia solanacearum (Smith 1896) were proposed.

The Phylogeny of the Genera Chryseomonas, Flavimonas, and Pseudomonas Supports Synonymy of These Three Genera

“…There was a good correlation between rRNA groups I to V and groups 1 to 5 based on cellular fatty acids and quinone systems. In the past two decades Pseudomonas species belonging to rRNA groups I1 to V or fatty acid and quinone groups 2 to 9 have been transferred to other genera (10,13,14,16,17,19,20). At this time, the genus Pseudomonas is restricted to Palleroni rRNA group I, and only the group I strains belong to the genus Pseudomonas sensu stricto.…”

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

“…The B. mallei mutant strains used in this study were derivatives of ATCC 23344, a highly pathogenic clinical isolate and type strain of the species [18][19][20]. B. mallei SR1, (BMAA0437-BMAA0497), is a sucrose-resistant mutant that harbors an IS407A-mediated deletion of a 78-kb region of chromosome 2 encompassing the sacB gene [19].…”

Pseudomonas aeruginosa under Phosphorus-Poor Culture Conditions: Phospholipid-Poor Bacterial Membranes, and Susceptibility to Antibacterial Chemicals, High Temperature and Low pH.

“…PE-1 and OL-1 are identical to PE and OL, respectively. PE-2 and OL-2 contain 2-hydroxy fatty acids 37) . OLs resulted in only minor changes in the susceptibility to various antibiotics.…”

“…The second development for two-dimensional TLC was carried out in solvent system II (chloroform-methanol-water [65:23:4, vol/vol]). Spots on the plates were visualized with 0.3% ninhydrin-buthanolacetic acid solution 31) , a-naphtol reagent 38) , Dittmer reagent 8) , or 50% H 2 SO 4 and identified by referring to standard substances and data in previous reports 36,37) . For analyses of fatty acids and amino acids of OLs, the lipid purified from a spot on a TLC plate was acid-hydrolyzed, and the n-hexane-soluble fraction and HCl-soluble fraction were examined by high pressure liquid chromatography (HPLC) as described previously 24,26) .…”