The Phylogeny of the Genera Chryseomonas, Flavimonas, and Pseudomonas Supports Synonymy of These Three Genera

Abstract

The 16s rRNA sequences of Chryseomonas Zuteola, the type species of the genus Chryseomonas, and Flavimonas oryzihabitans, the type species of the genus Flavimonas, were determined. These sequences were compared with the sequences of 27 representative strains of the genus Pseudomonus. C. Zuteola and F. oryzihabitans were located in the cluster that contains Pseudomonas aemginosa, the type species of genus Pseudomonas Migula 1894, and the levels of 16s rRNA sequence homology between P. aemginosa and the other two species were more than 93.9%. All of the strains of the genus Pseudomonas sensu stricto whose sequences have been determined were included in the P. aemginosa cluster. These results suggested that Chryseomonas, Flavimonas, and Pseudomonas are synonymous, and we concluded that Chryseomonas and Flavimonas are junior subjective synonyms of Pseudomonas.

Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea

“…This resulted from the ambiguous definition of the genus Pseudomonas taxon as 'polarly flagellated strictly aerobic rods with a respiratory type of metabolism in which oxygen is used'. The heterogeneity of the genus Pseudomonas was significantly resolved by extensive taxonomic studies based on phenotypic (Sneath et al, 1981;Stanier et al, 1966) and genotypic tests (Anzai et al, 1997(Anzai et al, , 2000Champion et al, 1980;Moore et al, 1996;Palleroni et al, 1972Palleroni et al, , 1973Sands et al, 1970). In particular, analyses of 16S rRNA sequences contributed to the elucidation of the natural relationships of species of the genus Pseudomonas at the intrageneric level and led to the significant redefinition and restriction of the genus Pseudomonas sensu stricto.…”

“…Phylogenetic analysis based on 16S rDNA sequences has been recognized as the most important method for inferring relationships of the genus Pseudomonas (Moore et al, 1996;Anzai et al, 1997Anzai et al, , 2000. According to Anzai et al (2000), 57 valid or invalid species among 128 species of the genus Pseudomonas were affiliated to the genus Pseudomonas sensu stricto and were divided into two major groups and six subgroups.…”

Pseudomonas thermotolerans sp. nov., a thermotolerant species of the genus Pseudomonas sensu stricto

“…Z76651) and slightly less similarity to the sequences of P. stutzeri, P. balearica, Pseudomonas resinovorans and other species of the ' P. aeruginosa and P. resinovorans phylogenetic lineages ' . The similarities of the 16S rDNA sequence of strain CM3 T to those of Pseudomonas species were within the range of 16S rRNA gene sequence similarities observed between the species of Pseudomonas (93n7-99n9 %) Anzai et al, 1997Anzai et al, , 2000. The inferred phylogenetic relationships of strain CM3 T , derived from evolutionary distance estimations and least-squares clustering, neighbour-joining, maximum-likelihood and analysis of the 16S rRNA gene sequences from representative species of the major phylogenetic lineages within the γ-Proteobacteria (Fig.…”

Mucilaginibacter roseus sp. nov., isolated from a freshwater river

“…Primers FD1 (59-AGAGTTTGATCCTGGCTCAG-39) and RD1 (59-AAGGAGGTGATCCAG CC-39) were used for amplification of bacterial 16S rRNA genes by PCR (Weisburg et al, 1991;Anzai et al, 1997). These primers correspond to nucleotide positions 8-27 and 1524-1540 of the Escherichia coli 16S rRNA gene, respectively, and can be used for amplifying a nearly full-length 16S rRNA gene.…”

“…These primers correspond to nucleotide positions 8-27 and 1524-1540 of the Escherichia coli 16S rRNA gene, respectively, and can be used for amplifying a nearly full-length 16S rRNA gene. The PCR product was purified, and direct sequencing was performed by using sequencing primers FD1, RD1, 520F and 800R (Weisburg et al, 1991;Anzai et al, 1997) with a DNA sequencer (ABI Prism 3730; Applied Biosystems). The sequenced length of the 16S rRNA gene was 1418 bp for strain TTM-1 T and this gene sequence was compared to those available from the EzTaxon-e server (Kim et al, 2012b), the Ribosomal Database Project (Cole et al, 2009) and the GenBank database (http://blast.ncbi.nlm.nih.gov/Blast.cgi.).…”