Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Ulrich, Ricky L.; Amemiya, Kei; Waag, David M.; Roy, Chad J.; DeShazer, David

doi:10.1016/j.vaccine.2004.10.017

Cited by 52 publications

(66 citation statements)

References 30 publications

(36 reference statements)

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“…However, purchasing and handling of Syrian hamsters is costlier than mice. The BALB/c mouse model has been effective only when the inoculations were performed by aerosol [7,8,11]. Our results suggest that relatively inexpensive CD1 mice can be used to study B. mallei pathogenicity using intraperitoneal inoculation.…”

Section: Discussionmentioning

confidence: 87%

“…A sucrose-resistant strain of B. mallei, SR1, which harbors an IS407A-mediated deletion in sacB gene has been used in past as the platform in making other mutants [10,11]. We constructed an isogenic DsacB mutant strain of B. mallei that can also be used as a platform to delete targeted genes.…”

Section: Discussionmentioning

confidence: 99%

“…These two immunoglobulins were found in almost equal proportions suggesting that both Th1 and Th2 responses were triggered. Ulrich et al [11] showed that when BALB/c mice were inoculated aerogenically with a non-capsulated mutant of B. mallei, serum IgG1 and IgG2a are induced in equal proportions, but when the vaccinated animals were boosted (revaccinated), a greater proportion of serum IgG1 was elicited. In the same study, when BALB/c mice were aerogenically inoculated with a branched-chain amino acid auxotroph, a greater proportion of serum IgG2a was induced.…”

Section: Discussionmentioning

confidence: 99%

“…Inactivation of individual genes by allelic exchange or transposon mutagenesis has been the most precise way of elucidating the specific roles of genes in the pathogenicity of a bacterium. B. mallei strain SR1 was previously used as the platform in making genetically-defined deletion mutants of this bacterium [10,11]. Strain SR1 was resistant to sucrose, a feature that was used as a non-antibiotic selection marker in these genetic manipulations.…”

Section: Subject Termsmentioning

confidence: 99%

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A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 mice

Bandara

DeShazer

Inzana

et al. 2008

Microbial Pathogenesis

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Subject Termsmentioning

confidence: 99%

See 2 more Smart Citations

A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 mice

Bandara

DeShazer

Inzana

et al. 2008

Microbial Pathogenesis

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“…Mice have proven to be valuable models for Burkholderia vaccine development (Fritz et al, 2000;Jeddeloh et al, 2003;Lever et al, 2003;Ulrich et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Pathological findings and diagnostic implications of a rhesus macaque (Macacca mulatta) model of aerosol exposure to Burkholderia mallei (glanders)

Yingst

Facemire

Chuvala

et al. 2015

Journal of Medical Microbiology

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Burkholderia mallei is a Gram-negative bacillus that causes a pneumonic disease known as glanders in equids and humans, and a lymphatic infection known as farcy, primarily in equids. With the potential to infect humans by the respiratory route, aerosol exposure can result in severe, occasionally fatal, pneumonia. Today, glanders infections in humans are rare, likely due to less frequent contact with infected equids than in the past. Acutely ill humans often have non-specific clinical signs and in order to diagnose cases, especially in scenarios of multiple cases in an unexpected setting, rapid diagnostics for B. mallei may be critical. The pathogenesis of acute glanders in the rhesus macaque (Macaca mulatta) was studied as an initial effort to improve diagnostic methods. In the study described here, the diagnostic techniques of PCR, culture and histopathology were compared. The results indicated that PCR may provide rapid, non-invasive diagnosis of glanders in some cases. As expected, PCR results were positive in lung tissue in 11/12 acutely infected rhesus macaques, but more importantly in terms of diagnostic algorithm development, PCR results were frequently positive in non-invasive samples such as broncho-alveolar lavage or nasal swabs (7/12) and occasionally in blood (3/12). However, conventional bacterial culture failed to recover bacteria in many of these samples. The study showed that the clinical presentation of aerosol-exposed rhesus macaques is similar to descriptions of human glanders and that PCR has potential for rapid diagnosis of outbreaks, if not individual cases.

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Burkholderia mallei and Glanders

Khakhum

Tapia

Torres

2019

Defense Against Biological Attacks

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Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Cited by 52 publications

References 30 publications

A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 mice

A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 mice

Pathological findings and diagnostic implications of a rhesus macaque (Macacca mulatta) model of aerosol exposure to Burkholderia mallei (glanders)

Burkholderia mallei and Glanders

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