Proposal for effective treatment of Shiga toxin-producing Escherichia coli infection in mice

Amran, Muhammad Yunus; Fujii, Jun; Kolling, Glynis L.; Villanueva, Sharon Y. A. M.; Kainuma, Mosaburo; Kobayashi, Hideyuki; Hideko, Kameyama; Yoshida, Shin Ichi

doi:10.1016/j.micpath.2013.09.008

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Other subtypes of Stx2 have been reported to produce neurological compromise. It has been reported that Stx2c and Stx2d caused damaged in the central nervous system (neuronal and endothelial ultrastructural alterations) and neurological symptoms (encephalopathy syndrome) in mice experimental models ( Fujii et al, 1994 ; Amran et al, 2013 ), similar to Stx2a as it was observed in the present work and in a previous study by our group ( Tironi-Farinati et al, 2013 ). In addition Stx2e induced the breakdown of the BBB and neurological disturbance in a porcine model ( Meisen et al, 2013 ), a similar finding to our results.…”

Section: Discussionsupporting

confidence: 90%

Sub-Lethal Dose of Shiga Toxin 2 from Enterohemorrhagic Escherichia coli Affects Balance and Cerebellar Cythoarquitecture

et al. 2016

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Shiga toxin producing Escherichia coli may damage the central nervous system before or concomitantly to manifested hemolytic–uremic syndrome symptoms. The cerebellum is frequently damaged during this syndrome, however, the deleterious effects of Shiga toxin 2 has never been integrally reported by ultrastructural, physiological and behavioral means. The aim of this study was to determine the cerebellar compromise after intravenous administration of a sub-lethal dose of Shiga toxin 2 by measuring the cerebellar blood–brain barrier permeability, behavioral task of cerebellar functionality (inclined plane test), and ultrastructural analysis (transmission electron microscope). Intravenous administration of vehicle (control group), sub-lethal dose of 0.5 and 1 ηg of Stx2 per mouse were tested for behavioral and ultrastructural studies. A set of three independent experiments were performed for each study (n = 6). Blood–brain barrier resulted damaged and consequently its permeability was significantly increased. Lower scores obtained in the inclined plane task denoted poor cerebellar functionality in comparison to their controls. The most significant lower score was obtained after 5 days of 1 ηg of toxin administration. Transmission electron microscope micrographs from the Stx2-treated groups showed neurons with a progressive neurodegenerative condition in a dose dependent manner. As sub-lethal intravenous Shiga toxin 2 altered the blood brain barrier permeability in the cerebellum the toxin penetrated the cerebellar parenchyma and produced cell damaged with significant functional implications in the test balance.

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Section: Discussionsupporting

confidence: 90%

Sub-Lethal Dose of Shiga Toxin 2 from Enterohemorrhagic Escherichia coli Affects Balance and Cerebellar Cythoarquitecture

et al. 2016

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“…Ciprofloxacin raises the production and release of Stx in vitro and is associated with a higher mortality rate in pigs ( 33 ). Other studies have shown that some antibiotics such as azithromycin might be associated with a decrease of the production and release of Stx in vitro ( 37 ) and with favorable outcomes in in vivo studies in piglets and mice ( 33 , 38 ). Finally, during a 2011 outbreak of EHEC O104 infection in Germany, a patient treated by ciprofloxacin plus imipenem unexpectedly had a better prognosis than all others ( 14 ).…”

Section: Discussionmentioning

confidence: 99%

EnterohemorrhagicEscherichia coliHybrid Pathotype O80:H2 as a New Therapeutic Challenge

Soysal¹,

Mariani‐Kurkdjian²,

Smail³

et al. 2016

Emerg. Infect. Dis.

103

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“…18 The efficacy of azithromycin against STEC has been demonstrated in mice. 19 In contrast, ciprofloxacin treatment has been shown to increase stress and the production of Stx. 20 The survival rate of affected neonatal pigs treated with azithromycin was higher than that of ciprofloxacin-treated animals and untreated controls.…”

Section: Discussionmentioning

confidence: 99%

Hemolytic uremic syndrome following complicated appendicitis in a child: what is the missing link?

et al. 2021

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We herein describe an 18-month-old boy who underwent initially successful surgical and antibiotic treatment of complicated appendicitis with postoperative occurrence of hemolytic uremic syndrome (HUS). This complication was due to Shiga toxin-producing Escherichia coli (STEC) found secondarily in rectal swabs but not in the peritoneal cavity. The literature indicates that a causal link may exist between these two entities, and HUS could be considered an iatrogenic complication of appendicitis management due to a multimodal stress effect in non-symptomatic STEC carriers.

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Proposal for effective treatment of Shiga toxin-producing Escherichia coli infection in mice

Cited by 6 publications

References 25 publications

Sub-Lethal Dose of Shiga Toxin 2 from Enterohemorrhagic Escherichia coli Affects Balance and Cerebellar Cythoarquitecture

Sub-Lethal Dose of Shiga Toxin 2 from Enterohemorrhagic Escherichia coli Affects Balance and Cerebellar Cythoarquitecture

EnterohemorrhagicEscherichia coliHybrid Pathotype O80:H2 as a New Therapeutic Challenge

Hemolytic uremic syndrome following complicated appendicitis in a child: what is the missing link?

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