EnterohemorrhagicEscherichia coliHybrid Pathotype O80:H2 as a New Therapeutic Challenge

Abstract

This emerging clonal group harbors the extraintestinal virulence–associated plasmid pS88 and can induce invasive infections and death.

Unusual severe case of hemolytic uremic syndrome due to Shiga toxin 2d-producing E. coli O80:H2

“…Moreover, the Stx2 d gene is known to cause a more severe course of disease. This together with the rare eae ξ and the plasmid of the O80:H2 STEC, which showed high resemblance with the previous described plasmid associated with extra intestinal virulence, resulted in an increased association with HUS [8]. Only recently, Soysal et al described the STEC serotype O80 as a new therapeutic challenge in patients with STEC-HUS due to its capacity to cause a bacteremia [8].…”

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

“…Another antibiotic‐resistant hybrid STEC serotype, O80:H2, was isolated in France from 54 patients between 2005 and 2014 (Soysal et al, ). These STEC isolates were found to carry several variants of the stx2 gene, a rare variant of the intimin gene (eae‐ξ) and genetic determinants related to the pS88 plasmid associated with extraintestinal‐virulence pathogenic E. coli (ExPEC), avian pathogenic E. coli (APEC) and E. coli strains that cause neonatal meningitis (NMEC; Soysal et al, ).…”

“…Another antibiotic‐resistant hybrid STEC serotype, O80:H2, was isolated in France from 54 patients between 2005 and 2014 (Soysal et al, ). These STEC isolates were found to carry several variants of the stx2 gene, a rare variant of the intimin gene (eae‐ξ) and genetic determinants related to the pS88 plasmid associated with extraintestinal‐virulence pathogenic E. coli (ExPEC), avian pathogenic E. coli (APEC) and E. coli strains that cause neonatal meningitis (NMEC; Soysal et al, ). Although STEC O80:H2 was found to be resistant to amoxicillin, nalidixic acid, cotrimoxazole and kanamycin with 52% of the isolates being resistant to all four antibiotics, subsequent in vitro studies demonstrated that this STEC was susceptible to azithromycin with no induction of Stx suggesting that this antibiotic could probably have been used to effectively treat patients (Soysal et al, ).…”

“…Another antibiotic-resistant hybrid STEC serotype, O80:H2, was isolated in France from 54 patients between 2005 and 2014 (Soysal et al, 2016). These STEC isolates were found to carry several variants of the stx2 gene, a rare variant of the intimin gene (eae-ξ) and…”

“…STEC persist at the recto-anal junction (RAJ) of cattle GIT and survive in manure for several months (Kudva, 2012;Kudva & Dean-Nystrom, 2011;Naylor et al, 2003). STEC also possess a plastic genome as evidenced by the step-wise evolution (loss and gain of certain genetic materials) of pathogenic O157 from a non-toxigenic E. coli O55:H7 serotype (Wick, Qi, Lacher, & Whittam, 2005) and the emergence of new AR-STEC genotypes (Frank et al, 2011;Soysal et al, 2016). Given the ability of STEC to persist over an extended period of time, to assimilate foreign DNA and that HGT can occur in STEC as in other bacteria (Ahmed & Shimamoto, 2015;von Wintersdorff et al, 2016), there is a possibility that STEC may acquire antibiotic resistance genes during its persistence in the bovine gut or in manure in the environment.…”