Propolis Suppresses CdCl&lt;sub&gt;2&lt;/sub&gt;-Induced Cytotoxicity of COS7 Cells through the Prevention of Intracellular Reactive Oxygen Species Accumulation

Kamiya, Tetsuro; Izumi, Misato; Hara, Hirokazu; Adachi, Tetsuo

doi:10.1248/bpb.b12-00062

Cited by 20 publications

(8 citation statements)

References 34 publications

(51 reference statements)

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“…Moreover, the co-treatment with antioxidant propolis significantly restored cell viability and reduced oxidant productions by suppressing Hcy toxicity. Previous studies have also revealed that the phenolic and flavonoid compounds of propolis, which play a crucial role in antioxidant capacity, have exhibited free radical scavenging activity and inhibited ROS formation in a dose-dependent manner [44,48,49]. It might be suggested that inhibition of Hcy-induced cell death is occurred by the reduction of intracellular ROS production.…”

Section: Discussionmentioning

confidence: 99%

Turkish propolis supresses MCF-7 cell death induced by homocysteine

Tartik

Darendelioğlu

Aykutoğlu

et al. 2016

Biomedicine & Pharmacotherapy

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Section: Discussionmentioning

confidence: 99%

Turkish propolis supresses MCF-7 cell death induced by homocysteine

Tartik

Darendelioğlu

Aykutoğlu

et al. 2016

Biomedicine & Pharmacotherapy

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“…Kamiya with co-workers showed that CdCl 2 induces cytotoxicity via intracellular ROS accumulation in COS7 cells. Pretreatment with EEP for 1 h significantly reduced intracellular ROS accumulation and suppressed CdCl 2 -induced cytotoxicity [ 23 ]. Da Silveira et al showed that ethanolic extract of yellow Brazilian propolis used at concentrations 1, 3, 10, and 30 mg/kg as dietary supplement for Wistar rats reduced the production of nitric oxide and malondialdehyde without changing level of total antioxidants, catalase, and superoxide dismutase, induced by behavioral stress [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of aqueous, polyethylene glycol-aqueous and ethanolic propolis extracts: antioxidant and mitochondria modulating properties

Kubilienė

Jekabsone

Žilius

et al. 2018

BMC Complement Altern Med

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BackgroundPropolis is multicomponent substance collected by honeybees from various plants. It is known for numerous biological effects and is commonly used as ethanolic extract because most of active substances of propolis are ethanol-soluble. However, water-based propolis extracts could be applied more safely, as this solvent is more biocompatible. On the other hand, water extracts has significantly smaller range and quantity of active compounds. The extraction power of water could be enhanced by adding co-solvent which increases both solubility and penetration of propolis compounds. However, variation of solvents results in different composition of active substances that might have distinct effects. The majority of biological effects of propolis are attributed to the antioxidant properties of its active compounds. Antioxidant effect might be a result of either direct scavenging of ROS or modulation of ROS producing organelle activity. Therefore, the aim of this study was to investigate and compare chemical composition, antioxidant properties and effects on mitochondrial respiration of aqueous (AqEP), polyethylene glycol-aqueous (Pg-AqEP) and ethanolic (EEP) propolis extracts.MethodsChemical composition of propolis extracts was determined using HPLC and Folin-Ciocalteu method. Ability to neutralize H2O2 and intracellular ROS concentration in C6 glioma cells were determined fluorometrically by using 10-acetyl-3,7-dihydroxyphenoxazine and 2′,7′-dichlorofluorescein diacetate, respectively. Mitochondrial superoxide generation was assessed under fluorescent microscope by using MitoSOX Red. Oxygen uptake rates of mitochondria were recorded by high-resolution respirometer Oxygraph-2 k.ResultsOur data revealed that phenolic acids and aldehydes make up 40–42% of all extracted and identified compounds in AqEP and Pg-AqEP and only 16% in EEP. All preparations revealed similar antioxidant activity in cell culture medium but Pg-AqEP and EEP demonstrated better mitochondrial superoxide and total intracellular ROS decreasing properties. At higher concentrations, AqEP and EEP inhibited mitochondrial respiration, but Pg-AqEP had concentration-dependent mitochondria-uncoupling effect.ConclusionsAqueous and non-aqueous propolis extracts differ by composition, but all of them possess antioxidant properties and neutralize H2O2 in solution at similar efficiency. However, both Pg-AqEP and EEP were more effective in decreasing intracellular and intramitochondrial ROS compared to AqEP. At higher concentrations, these preparations affect mitochondrial functions and change energy production in C6 cells.

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“…Cadmium exposure to primary culture of rat proximal tubular cells (1.25–40 μM CdAc 2 for 12 h), demonstrated a concentration and time-dependent loss of cell viability (mostly apoptotic). Cytotoxicity was also observed in kidney tubular epithelial cells (Cos7) exposed to CdCl 2 (0–80 μg/mL) for 24 h. This cytotoxicity was caused by Cd-induced oxidative stress and could be inhibited by antioxidant treatment of these cells with Propolis, a natural antioxidant product produced by honey bees [36]. The ability of the antioxidant N -acetylcysteine (NAC) to partially reverse apoptotic cell death implicates a definite role of oxidative stress in the apoptotic mechanism mediated by Cd [37].…”

Section: Cd-induced Pathologies: a Central Role For Oxidative Stressmentioning

confidence: 99%

Cadmium-Induced Pathologies: Where Is the Oxidative Balance Lost (or Not)?

Nair

DeGheselle

Smeets

et al. 2013

IJMS

264

157

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Over the years, anthropogenic factors have led to cadmium (Cd) accumulation in the environment causing various health problems in humans. Although Cd is not a Fenton-like metal, it induces oxidative stress in various animal models via indirect mechanisms. The degree of Cd-induced oxidative stress depends on the dose, duration and frequency of Cd exposure. Also the presence or absence of serum in experimental conditions, type of cells and their antioxidant capacity, as well as the speciation of Cd are important determinants. At the cellular level, the Cd-induced oxidative stress either leads to oxidative damage or activates signal transduction pathways to initiate defence responses. This balance is important on how different organ systems respond to Cd stress and ultimately define the pathological outcome. In this review, we highlight the Cd-induced oxidant/antioxidant status as well as the damage versus signalling scenario in relation to Cd toxicity. Emphasis is addressed to Cd-induced pathologies of major target organs, including a section on cell proliferation and carcinogenesis. Furthermore, attention is paid to Cd-induced oxidative stress in undifferentiated stem cells, which can provide information for future therapies in preventing Cd-induced pathologies.

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Propolis Suppresses CdCl<sub>2</sub>-Induced Cytotoxicity of COS7 Cells through the Prevention of Intracellular Reactive Oxygen Species Accumulation

Cited by 20 publications

References 34 publications

Turkish propolis supresses MCF-7 cell death induced by homocysteine

Turkish propolis supresses MCF-7 cell death induced by homocysteine

Comparison of aqueous, polyethylene glycol-aqueous and ethanolic propolis extracts: antioxidant and mitochondria modulating properties

Cadmium-Induced Pathologies: Where Is the Oxidative Balance Lost (or Not)?

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