Propofol attenuates formation of lipid peroxides in tourniquet-induced ischaemia-reperfusion injury.

Abstract

We studied 20 adult ASA I patients undergoing elective peripheral surgery allocated randomly to one of two groups. In the propofol group (n = 9) anaesthesia was induced with propofol and fentanyl followed by continuous infusion of propofol. In the control group (n = 11), after induction of anaesthesia with thiopentone and fentanyl, anaesthesia was maintained with isoflurane. Concentrations of lipid peroxides in both plasma and muscle tissue samples were measured as thiobarbituric acid-reacting substances (TBARS). Plasma TBARS concentrations increased significantly in the control group at 1, 5, 15, 30 and 45 min after release of the tourniquet to mean 1.83 (SD 0.13), 2.00 (0.12), 2.25 (0.14), 2.30 (0.12) and 2.41 (0.14) mumol litre-1, respectively, compared with pre-reperfusion values (1.64 (0.14) mumol litre-1). In the propofol group this was significant only at 30 min (1.85 (0.03) vs 1.74 (0.04) mumol litre-1). TBARS concentrations of reperfused muscle tissue were significantly higher than pre-reperfusion concentrations in the control group (70.30(10.06) vs 52.13 (5.73) nmol/g wet tissue). We conclude that propofol attenuated ischaemia-reperfusion-induced lipid peroxidation in the therapeutic doses used in anaesthesia.

Comparison between sevoflurane and isoflurane anesthesia in pig hepatic ischemia-reperfusion injury

“…Therefore, α-GST is considered to be a more sensitive marker of acute hepatic damage and its recovery than either AST or ALT [10,11]. (2) Plasma lipid peroxides were measured both to evaluate oxidative stress, which is one of the underlying mechanisms for IRI, and to enable the inhibitory effect of anesthetics on this stress to be evaluated [12,13]. Oxygen radicals, which cause cell damage via lipid peroxidation, are considered to be generated during liver ischemia-reperfusion [18,19].…”

The Effect of Dexmedetomidine and Propofol on Oxidative Stress and Antioxidizing System Studied on Liver Ischemia-reperfusion Model on Rats

“…Aarts et al has reported that propofol has a preventive role in cases such as coronary artery transplantation surgery, sepsis and burns, which of all lead into a decreased ontioxidising defense mechanism [13]. Also in some recent studies propofol has been reported to be a free radical scavenger due to its antioxidising property at anesthetic doses [21][22][23].…”

Ischaemic preconditioning attenuates haemodynamic response and lipid peroxidation in lower-extremity surgery with unilateral pneumatic tourniquet application: A clinical pilot study

“…4 A significant increase in MDA levels have been observed in the lung tissue during systemic inflammation induced by sepsis 22 and in plasma during lower-limb IR in patients. 5 The generation of MDA is believed to originate in the inflammatory cells. 4 In our study, the significant rise in plasma TBARS level in the T group compared with baseline values and not in the IP-T group supports the supposition that a lipid peroxidation process occurs during IR, and the IP attenuates this process.…”

“…4 Significant increase in TBARS level has been observed in the plasma of patients undergoing lower-limb IR. 5 Ischaemic preconditioning (IP) is defined as a brief period of ischaemia followed by tissue perfusion, thereby increasing ischaemic tolerance of tissue for a subsequent longer ischaemic period. This phenomenon was first described by Murry et al, 6 in a canine model of myocardial ischaemia.…”