Comparison between sevoflurane and isoflurane anesthesia in pig hepatic ischemia-reperfusion injury

Ishida, Hiroshi; Kadota, Yoshitami; Sameshima, T.; Nishiyama, Atsushi; Oda, Toshiyuki; Kanmura, Yuichi

doi:10.1007/s540-002-8093-x

Cited by 16 publications

(18 citation statements)

References 22 publications

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“…In this experiment, the MDA level in hepatic tissues after hepatic ischemia/reperfusion was shown to increase, which suggests that notable damage during hepatic ischemia/reperfusion is caused by free radicals. This observation is consistent with results from previous studies on ischemia/reperfusion [16,17]. During lipid peroxidation, some volatile saturated alkanes were also produced in addition to MDA.…”

Section: Discussionsupporting

confidence: 93%

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Liu¹,

Shi²,

Wang³

et al. 2012

J. Breath Res.

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The aim of this study was to determine the changes in the pentane concentration of expiratory gas as well as the relationship between this pentane concentration and hepatic oxidative stress during rabbit hepatic ischemia/reperfusion using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC/MS). 45 white male rabbits with body weights between 2.5 and 3.0 kg were randomly assigned to the following three groups: the 10 min ischemia group (group S); the 20 min ischemia group (group M); or the 30 min ischemia group (group L). Expiratory gases were collected prior to ischemia (T0) and for 1, 10, 20, 30, 60 and 120 min (T1-T6) following reperfusion. Pentane concentrations were determined using SPME and GC/MS. In addition, arterial blood samples were collected, and serum aminotransferase (AST) and malondialdehyde (MDA) concentrations were measured. In the three groups, the pentane concentrations of the expiratory gases at points T1 and T2 were significantly increased (P < 0.05) compared with those at point T0, and the serum AST and MDA concentrations at points T5 and T6 were also significantly increased (P < 0.05) compared with those at point T0. Therefore, the use of SPME in combination with GC/MS represents an improved anesthesia system that can be used to continuously measure the concentration of pentane in expiratory gases, which can reflect the degree of oxidative stress during hepatic ischemia/reperfusion.

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Section: Discussionsupporting

confidence: 93%

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Liu¹,

Shi²,

Wang³

et al. 2012

J. Breath Res.

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“…15,29 Further studies are needed to examine the effects of different concentrations of sevoflurane during the pretreatment on the subsequent protective effects of sevoflurane on the shedding of HS and Syn-1. 9,11,15,18 The reduction of the activation of various adhesion molecules during the reperfusion is likely to contribute to the protective effects of sevoflurane pretreatment on the hepatocytes. 1,27 Adhesion molecules are a class of small proteins that are also associated with the endothelial glycocalyx.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of sevoflurane in hepatic ischemia-reperfusion injury

Yuan

Zhao

et al. 2016

Int J Immunopathol Pharmacol

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The endothelial glycocalyx plays a critical role in the regulation of vascular structure and functions. Previous studies have demonstrated that sevoflurane, a volatile anesthetic, can preserve the endothelial glycocalyx in heart tissues against ischemia-reperfusion injury. However, little is known about the effects of sevoflurane pretreatment on the vascular structure and functions of liver tissues following ischemia-reperfusion injury. To this end, female Sprague-Dawley rats (n = 28) were anesthetized either with ketamine (80-120 mg/kg, i.p.) or with one minimum alveolar concentration (MAC) sevoflurane (2% v/v). Following in vivo hepatic ischemia procedure, the liver was isolated and reperfusion was produced. During the period of reperfusion, liver reperfusion samples were collected, and the concentrations of heparan sulfate and syndecan-1 (Syn-1), and the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzymes, were measured. The morphology of hepatocytes and endothelial glycocalyx were then assessed by using the light and electron microscopies, respectively. Ischemia-reperfusion increased the release of HS and Syn-1, and elevated the levels of ALT and AST in a time-dependent manner. However, sevoflurane pretreatment reduced the release of HS and Syn-1and attenuated the levels of ALT and AST, in a time-dependent manner, as compared with ketamine pretreatment. Furthermore, sevoflurane pretreatment decreased the shedding of endothelial glycocalyx and hepatocytes necrosis. Sevoflurane pretreatment preserved the endothelial glycocalyx in the liver tissue against ischemia-reperfusion injury. The effect appears to help protect hepatocytes against ischemia-reperfusion-induced necrosis.

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“…Volatile anesthetics such as isoflurane and sevoflurane were reported to have pre-conditioning or protective effects on the liver in ischemic re-perfusion or liver surgery models, but there are no reports regarding the pre-conditioning effect of propofol injection on hepatic surgery [6,[35][36][37]. In those studies, changes in expression of antioxidant proteins such as CAT, Cu-Zn SOD, and GST after anesthesia were not evaluated [36,38]. Antioxidant systems have not been sufficiently examined at molecular levels, and our findings suggest the presence of alterations in the liver protective effect of antioxidant proteins by general anesthetics.…”

Section: Discussionmentioning

confidence: 99%

Volatile and Intravenous Anesthesia Alter Rat Liver Proteins: Proteomic Time Course Analysis of Rat Liver Proteins

Watanabe¹

2012

TOPROTJ

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Abstract:Background: Our previous microarray study showed that sevoflurane anesthesia affects the expression of rat genes in multiple organs including the liver. In this study, we investigated whether liver protein expression was altered after propofol, sevoflurane, or isoflurane anesthesia. We also investigated differences in the time course of each drug 24 and 72 h after anesthesia.Methods: Rats were randomly assigned to four groups (non-anesthetized group and three groups anesthetized at each time point, n = 6 per group). A venous catheter was inserted into the caudal vein of all rats. Rats were anesthetized with each agent for 6 h, and the liver was obtained immediately after anesthesia. Proteomic analysis was performed.Results: About 4200 spots in each gel were discriminated, and at least 2619 spots were matched. Using LC-MS/MS, we identified 47 spots for propofol, 45 spots for sevoflurane, and 21 spots for isoflurane that were differentially expressed (p < 0.05) 0 h after anesthesia. The numbers of altered proteins were 14 and 19 in the isoflurane and sevoflurane groups, respectively, 72 h after anesthesia, but alterations in 40 proteins were seen in the propofol group 72 h after anesthesia. Conclusion:Volatile and intravenous anesthetics affected protein expression in the liver. Alterations were different for each drug, with isoflurane showing fewer altered proteins 0 h after anesthesia than the other two drugs. The time courses of those proteins were also different between individual anesthetics, suggesting fewer alterations in rat liver protein expression with volatile anesthetics than with propofol.

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Comparison between sevoflurane and isoflurane anesthesia in pig hepatic ischemia-reperfusion injury

Abstract: The extent of the hepatic IRI seen under sevoflurane anesthesia in pigs did not differ significantly from that seen under isoflurane, as judged from measurements of a number of parameters over a 240-min reperfusion period.

Cited by 16 publications

References 22 publications

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Protective effects of sevoflurane in hepatic ischemia-reperfusion injury

Volatile and Intravenous Anesthesia Alter Rat Liver Proteins: Proteomic Time Course Analysis of Rat Liver Proteins

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