Propidium iodide for making heterochromatin more evident in the C-banding technique

Lui, RL; Blanco,; Moreira‐Filho, Orlando; Margarido, Vladimir Pavan

doi:10.3109/10520295.2012.696700

Cited by 86 publications

(42 citation statements)

References 54 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metaphasic cells were obtained from the kidney (Bertollo et al 1978;Foresti et al 1993). Heterochromatin was revealed through C banding (Sumner 1972;Lui et al 2012) and nucleolar organizer regions (AgNORs) were revealed through silver nitrate impregnation (Howell and Black 1980). Chromosome types were classified as metacentric (m), submetacentric (sm), subtelocentric (st) or acrocentric (a) based on the arm relationship criteria proposed by Levan et al (1964).…”

Section: Methodsmentioning

confidence: 99%

Karyotypic diversity and chromosome evolution in the armored catfishes Callichthyinae (Siluriformes, Callichthyidae)

et al. 2014

View full text Add to dashboard Cite

The Callichthyinae subfamily is composed of five genera with a small number of described species. Molecular cytogenetics studies show the scarcity of records for this subfamily. The aim of the present study was to employ cytogenetic parameters in the analysis of three species belonging to the subfamily Callichthyinae sampled from the Paraná River (Brazil). Callichthys callichthys had 2n = 56 chromosomes (26 m-sm + 30 st-a); Lepthoplosternum pectorale had 2n = 64 chromosomes (8 m-sm + 56 st-a); and Hoplosternum littorale had 2n = 60 chromosomes (8 m-sm + 52 st-a). Regarding the location of the nucleolar organizer regions (NORs) (Ag-staining and 18S rDNA-FISH), different situations were found: single NORs in H. littorale and C. callichthys; multiple NORs in L. pectorale, with intra-individual and inter-individual variation. Heterochromatin was observed in the centromeric region in the chromosomes of the three species. Equilocal, interstitial bands were also found in H. littorale. Physical mapping of 5S ribosomal genes by FISH shows eight 5S rDNA sites in C. callichthys; four 5S rDNA sites in H. littorale and six 5S rDNA sites in L. pectorale. Using cytogenetic markers (diploid number, chromosome formula, NORs, heterochromatin distribution pattern and 5S and 18S rDNA sites), the chromosomal evolution in Callichthyinae is presented: Callichthys shows mostly basal chromosomal conditions, with mostly derived chromosomal conditions verified for the Hoplosternum-Dianema clade and Megalechis-Lepthoplosternum clade.

show abstract

Section: Methodsmentioning

confidence: 99%

Karyotypic diversity and chromosome evolution in the armored catfishes Callichthyinae (Siluriformes, Callichthyidae)

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The fundamental number (FN) was calculated considering metacentric (m), submetacentric (sm) and subtelocentric (st) chromosomes as having two arms, and acrocentric (a) chromosomes as having only one arm. The heterochromatic pattern was determined by Sumner (1972), with modifications (Lui et al, 2012a) in slides previously analyzed with Giemsa staining (sequential analysis). The Ag-NORs were identified using impregnation by silver nitrate (Howell and Black, 1980), and this preparation was performed on metaphases previously stained by Giemsa and C-banded (sequential analysis).…”

Section: Methodsmentioning

confidence: 99%

Karyotypic variation of Glanidium ribeiroi Haseman, 1911 (Siluriformes, Auchenipteridae) along the Iguazu river basin

et al. 2015

View full text Add to dashboard Cite

The Iguazu river is a tributary of the left margin of the Paraná river, isolated from this basin about 22 million years ago with the appearance of the Iguazu Falls. The Iguazu river is characterized by high endemism due to two factors: its rugged topography and the old isolation caused by formation of the Iguazu Falls. This study analyzed cytogenetically a population of Glanidium ribeiroi collected in a region at the final stretch of this basin, by Giemsa staining, C-banding, impregnation by silver nitrate, and FISH with probes of 5S rDNA, 18S rDNA, telomeric sequence [TTAGGG] n , and [GATA] n repeats. The diploid number was equal to 58 chromosomes. The heterochromatin was present in the terminal region of almost all chromosomes. The Ag-NORs were simple and presented interstitially on the short arm of the submetacentric pair 14, which was confirmed by FISH with 18S rDNA probe. The 5S rDNA-FISH marked only the submetacentric pair 16 on the long arm in interstitial position. The FISH with [TTAGGG] n probe presented all telomeres labeled as expected, with an absence of Interstitial Telomeric Sequence (ITS). The repetitive [GATA] n sequence was dispersed throughout the genome, with preferential location in the terminal region of all chromosomes. The data obtained are discussed herein with other species of Auchenipteridae, and other previously analyzed populations of G. ribeiroi from the Iguazu river, verifying differences among these populations, which should be mainly related to the rugged topography of this basin.Keywords: geographical barriers, ribosomal DNA, Glanidium ribeiroi, Iguazu river. Variação cariotípica de Glanidium ribeiroi

show abstract

“…The heterochromatic distribution pattern was determined according to the protocol described by Sumner (1972), with modifications in the coloring step (Lui et al, 2012). The NORs were identified by AgNOR, described by Howell and Black (1980).…”

Section: Methodsmentioning

confidence: 99%

Chromosomal diversity in Hypostomus (Siluriformes, Loricariidae) with emphasis on physical mapping of 18S and 5S rDNA sites

Traldi¹,

Blanco²,

Vicari³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. We examined chromosomes of three species of the genus Hypostomus, in order to contribute to the understanding of the karyotype evolution of this group. Specimens of H. ancistroides and H. nigromaculatus displayed differences in karyotype formulas, distribution and location of heterochromatin and nucleolus organizer regions when compared to other populations of the same species. We made the first cytogenetic characterization of H. tapijara, an endemic species in the Ribeira de Iguape River. These specimens had 2n = 66 chromosomes, while H. ancistroides showed 2n = 68 and H. nigromaculatus 2n = 76 chromosomes. Physical mapping of 18S and 5S rDNA sites of the three species showed simple, multiple and syntenic clusters. Synteny of ribosomal sites was found in H. ancistroides and H. tapijara, and an interspersed pattern between these sites in all chromosomes bearing the synteny was observed. We conclude that the genus Hypostomus has a high chromosome complexity that is accompanied by great morphological variation. It is evident that this group comprises an interesting model for understanding the chromosome evolution of Neotropical ichthyofauna.

show abstract

Propidium iodide for making heterochromatin more evident in the C-banding technique

Cited by 86 publications

References 54 publications

Karyotypic diversity and chromosome evolution in the armored catfishes Callichthyinae (Siluriformes, Callichthyidae)

Karyotypic diversity and chromosome evolution in the armored catfishes Callichthyinae (Siluriformes, Callichthyidae)

Karyotypic variation of Glanidium ribeiroi Haseman, 1911 (Siluriformes, Auchenipteridae) along the Iguazu river basin

Chromosomal diversity in Hypostomus (Siluriformes, Loricariidae) with emphasis on physical mapping of 18S and 5S rDNA sites

Contact Info

Product

Resources

About