Properties of Selected Hemicellulases of a Multi-Enzymatic System fromPenicillium funiculosum

Abstract

A multi-enzymatic system from Penicillium funiculosum displayed -L-arabinofuranosidase, endo-1,4--Dxylanase, -D-xylosidase and endo-1,3-1,4--D-glucanase activities at high levels over a wide acidic pH range of 2.0 to 5.5. Moreover, the pH stability was particularly extended over the wide range of pH of 2.0 to 8.0 with endo-1,3-1,4--D-glucanase and endo-1,4--D-xylanase; however, -L-arabinofuranosidase and -D-xylosidase exhibited higher stability in the pH range of 2.0 to 5.5. The results indicate that the optimal temperature of -L-arabinofuranosidase (65 C) and -D-xylosidase (70 C) as well as their thermal stability were higher than those of endo-1,3-1,4--D-glucanase (60 C) and endo-1,4--D-xylanase (50 C). Although V maxapp of -Dxylosidase and endo-1,4--D-xylanase was higher than that of -L-arabinofuranosidase and endo-1,3-1,4--Dglucanase, respectively, their catalytic efficiency was lower. High levels of ferulolyl esterase, -D-galactosidase, -D-mannosidase and endo-1,4--D-mannanase activities were also detected in the multi-enzymatic system. The overall features of the multi-enzymatic system from P. funiculosum reveal its potential for degrading and modifying plant cell walls from a variety of food and feedstuffs.

Effects of dietary supplementation of exogenous multi-enzyme mixture containing carbohydrases and phytase on growth performance, energy and nutrient digestibility, and selected mucosal gene expression in the small intestine of weanling pigs fed nutrient deficient diets

“…One FTU is defined as the amount of enzyme that liberates one micromole of inorganic ortho-phosphate from phytic acid per minute at pH 5.5 and 37°C (Engelen et al 1994). In addition to endo-β-1,4-xylanase, endo-β-1,3(4)-glucanase and phytase activities, other enzymes (cellulases, pectinases, mannanase, and others) are also present and active in the preparation (Karboune et al 2008(Karboune et al , 2009. The presence of the enzyme preparation in the supplemented diets was confirmed by analyzing the corresponding xylanase and phytase activities.…”

Wheat-barley-rye- or corn-fed growing pigs respond differently to dietary supplementation with a carbohydrase complex

“…the amount of enzyme, which hydrolyzes wheat arabinoxylans, reducing the viscosity of the solution to give a change in relative ñuidity of 1 arbitrary unit-min"û nder the assay conditions (pH 5.5 and 30°C) for xylanase activity, and the quantity of enzyme, which hydrolyzes barley ß-glucan (bound to a chromophore), releasing ethanol-soluble oligomers to give an absorbance of 0.820 units at 590 nm for endo-ß-l,3(4)-glucanase activity. In addition to endo-ß-l,4-xylanase and endoß-l,3(4)-glucanase activities, other enzymes (cellulases, pectinases, mannanase, and others) are also active in the preparation (Karboune et al, 2008(Karboune et al, , 2009. The presence of the enzyme preparation in the supplemented diets was confirmed by analyzing the corresponding ß-glucanase and xylanase activities (Cosson et al, 1999).…”

High-level expression of a novel Penicillium endo-1,3(4)-β-d-glucanase with high specific activity in Pichia pastoris

“…Penicillium [20] has been proved to be an excellent producer of cellulases and hemicellulases for industrial application as well as the genera Aspergillus and Trichoderma. Several endo-b-1,4-glucanases from Penicillium pinophilum KMJ601 [19], P. purpurogenum [22], and P. echinulatum [32] have been reported, but all of them only have the ability to hydrolyze the b-(1 ?…”