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Introduction. Cellulose-containing parts of herbs are an excellent source of alternative energy and can be used to produce biological ethanol. The present research aims at improving this fundamental and promising area of biotechnology. It introduces a new consortium of microorganisms that can saccharify while fermenting the substrate. Study objects and methods. The research featured technical cellulose obtained from Miscanthus sinensis using hydrotropic delignification and oxidation with pertrifluoroacetic acid. The ethanol content in the culture liquid was determined using an Agilent 7890B gas chromatograph with a flame ionization detector. The biocompatibility of the strains was studied by growing a direct co-culture in a dense nutrient medium. Results and discussion. The research objective was to create a new microbial consortium for the single-step production of bioethanol from Miscanthus sinensis cellulose. A set of biocompatibility experiments and cultivation conditions made it possible to select the optimal producers. The two developed microbial consortia required optimal compositions of culture media, which were determined by varying the ratio of components and measuring the yield of ethanol in the resulting culture liquid. Conclusion. The best consortium for Miscanthus sinensis cellulose consisted of Pichia stipites Y7124, Candida shehatae NCL3501, Kluyveromyces marxianus Y-4290, and Zymomonas mobilis 113 at a ratio of 1:1:1:1. The optimal parameters of bioethanol production included: temperature = 35 ± 1°C, pH = 5.2, time = 16 ± 1 h. The most efficient culture medium had the following composition (g/l): glucose – 5.0; peptone – 5.0; yeast extract – 0.4; K2HPO4 – 1.5; (NH)2 HPO4 – 1.5; MgSO4 – 0.5.
Introduction. Cellulose-containing parts of herbs are an excellent source of alternative energy and can be used to produce biological ethanol. The present research aims at improving this fundamental and promising area of biotechnology. It introduces a new consortium of microorganisms that can saccharify while fermenting the substrate. Study objects and methods. The research featured technical cellulose obtained from Miscanthus sinensis using hydrotropic delignification and oxidation with pertrifluoroacetic acid. The ethanol content in the culture liquid was determined using an Agilent 7890B gas chromatograph with a flame ionization detector. The biocompatibility of the strains was studied by growing a direct co-culture in a dense nutrient medium. Results and discussion. The research objective was to create a new microbial consortium for the single-step production of bioethanol from Miscanthus sinensis cellulose. A set of biocompatibility experiments and cultivation conditions made it possible to select the optimal producers. The two developed microbial consortia required optimal compositions of culture media, which were determined by varying the ratio of components and measuring the yield of ethanol in the resulting culture liquid. Conclusion. The best consortium for Miscanthus sinensis cellulose consisted of Pichia stipites Y7124, Candida shehatae NCL3501, Kluyveromyces marxianus Y-4290, and Zymomonas mobilis 113 at a ratio of 1:1:1:1. The optimal parameters of bioethanol production included: temperature = 35 ± 1°C, pH = 5.2, time = 16 ± 1 h. The most efficient culture medium had the following composition (g/l): glucose – 5.0; peptone – 5.0; yeast extract – 0.4; K2HPO4 – 1.5; (NH)2 HPO4 – 1.5; MgSO4 – 0.5.
Forests being mostly non-renewable, miscanthus seems to be a promising alternative energy resource. This study investigates the enzymatic hydrolysis of pretreated miscanthus biomass. The research featured Miscanthus sinensis Strictus biomass. The methods included raw material pretreatment, enzymatic hydrolysis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) of monosaccharide residues, and nuclear magnetic resonance (1H NMR) spectrometry of non-hydrolyzed lignin. A hammer mill with a particle size of 0.2–0.3 mm proved to be effective for preliminary mechanical processing of miscanthus. This type of treatment provided the maximal yield of carbohydrate-containing biomass (62.33 ± 1.87%). The optimal multi-enzyme composition included Cellulase Ultra obtained from Trichoderma reeseii, xylanase obtained from Thermomyces lanuginosus, and β-gluconase obtained from Myceliophtorafergusii. The fermentation temperature was 50 ± 1°C (72 ± 1 h). At these parameters, the conversion of holocellulose of miscanthus biomass was 96.0 ± 4.8%, and the yield of reducing substances was 97.00 ± 4.85%. The article introduces the optimal enzymatic hydrolysis parameters for pretreating Miscanthus sinensis Strictus biomass as a source of carbohydrate-containing substrates and describes their subsequent use for bacterial cellulose biosynthesis. The carbohydrate composition of hydrolysates included residual lignin, such polysaccharides as glucan and xylan, and various monosaccharides, namely arabinose, xylose, galactose, glucose, and uronic acids. Hydrolyzed miscanthus proved to be an effective renewable and environmentally friendly biodiesel.
МИНИСТЕРСТВО НАУКИ И ВЫСШЕГО ОБРАЗОВАНИЯ РФ Бийский технологический институт (филиал) федерального государственного бюджетного образовательного учреждения высшего образования «Алтайский государственный технический университет им. И.И. Ползунова» ТЕХНОЛОГИИ И ОБОРУДОВАНИЕ ХИМИЧЕСКОЙ, БИОТЕХНОЛОГИЧЕСКОЙ И ПИЩЕВОЙ ПРОМЫШЛЕННОСТИ Материалы XIV Всероссийской научно-практической конференциистудентов, аспирантов и молодых ученых с международным участием, посвященной 90-летнему юбилею академика Саковича Г.В. (1921 мая 2021 года, г. Бийск) Бийск Издательство Алтайского государственного технического университета им. И.И. Ползунова 2021 УДК 66.02(045) ББК 34.7 Т38 Т38 Технологии и оборудование химической, биотехнологической и пищевой промышленности: материалы XIV Всероссийской научно-практической конференции студентов, аспирантов и молодых ученых с международным участием, посвященной 90-летнему юбилею академика Саковича Г.В. (1921 мая 2021 года, г. Бийск) / Алт. гос. техн. ун-т, БТИ. -Бийск: Изд-во Алт. гос. техн. ун-та, 2021. -458 с. ISBN 978-5-9257-0327-4 В сборнике представлены материалы конференции в виде научных статей преподавателей, инженеров, аспирантов и студентов вузов России, а также работников научных и производственных объединений, подготовленных в рамках тематики XIV Всероссийской научно-практической конференции студентов, аспирантов и молодых ученых с международным участием «Технологии и оборудование химической, биотехнологической и пищевой промышленности», посвященной 90летнему юбилею академика Саковича Г.В. (1921 мая 2021 года, г. Бийск). Освещены актуальные вопросы по проблемам моделирования и интенсификации технологических процессов, подготовке кадров, оптимальному выбору рецептур, технологических режимов и оборудования с последующей экономической оценкой при их адаптации для промышленных предприятий. В сборнике материалы расположены по секциям: -химические технологии и аппаратурное оформление процессов; -биотехнологии и аппаратурное оформление процессов; -пищевые технологии и аппаратурное оформление процессов. УДК 66.02(045) ББК 34.7 Редакционная коллегия конференции «Технологии и оборудование химической, биотехнологической и пищевой промышленности» д.т.н., профессор А.Н. Блазнов к.х.н., доцент В.В. Будаева к.т.н., доцент И.Н. Павлов Часть докладов воспроизведена в виде, представленном авторами ISBN 978-5-9257-0327-4 БТИ АлтГТУ, 2021 3 МИНИСТЕРСТВО НАУКИ И ВЫСШЕГО ОБРАЗОВАНИЯ РФ Бийский технологический институт (филиал) ФГБОУ ВО «Алтайский государственный технический университет им. И.И. Ползунова»
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