Properties of endogenous, membrane-associated sialidase activity (N-acetylneuraminidase) of the goldfish visual system

Leskawa, Kenneth C.; Agranoff, Bernard W.

doi:10.1007/bf00965657

Cited by 5 publications

(1 citation statement)

References 47 publications

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“…For instance, the optimal pHs for zebrafish Neu3. Interestingly, it is reported that ganglioside sialidase in the retina and optic tectum of goldfish (Carassius auratus) has an optimal pH of 4.0 (28). Desialylation occurring in the optic tectum enhances synapse activity (29), whereas optic nerve crush, which results in regeneration of retinal ganglion cell axons, does not result in significant changes in endogenous membrane sialidase activity in either the retina or the optic tectum.…”

Section: Sialidases In Cypriniformesmentioning

confidence: 99%

Functional Characterization of Fish Sialidases and Their Diversity among Different Orders

Shiozaki

Oishi

Honda

2019

TIGG

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Sialidase catalyzes the removal of sialic acids from glycoconjugates. While the physiological functions of mammalian sialidases have been elucidated, sialidases in other vertebrates, including those in fish, are not well understood. Recently, increased genomic resources in fish have become available, which will facilitate research on fish sialidase.Here, three fish orders (Cypriniformes, Beloniformes and Perciformes) have been selected to compare the properties of their sialidases. A Neu1 knockdown in medaka by a morpholino oligo resulted in the accumulation of Sia α2-3 linked sialoglycoproteins, leading to malfunctions in heart beating, embryo growth, hatching, and swimming, indicating that lysosomal Neu1 is a crucial factor for embryogenesis.Fish Neu3 and Neu4 function varies between fish species. Medaka and tilapia Neu3a localize at the plasma membrane, as is seen in humans. Zebrafish Neu3.1 is an ER sialidase, with an optimal pH (pH 2.6). Neu4 shows high diversity in subcellular localization: medaka and zebrafish Neu4 are detected at the lysosome and ER, respectively. Surprisingly, tilapia Neu4 is detected at nucleus, which makes it the first nuclear sialidase to be identified. Moreover, importin has been identified as a regulator of tilapia Neu4 functions. These results demonstrate the diversity of sialidase functions among fish species and the requirement of further fish research.

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Section: Sialidases In Cypriniformesmentioning

confidence: 99%

Functional Characterization of Fish Sialidases and Their Diversity among Different Orders

Shiozaki

Oishi

Honda

2019

TIGG

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Regulation of glycolipid synthesis during differentiation of clonal murine muscle cells

Leskawa

Hogan

1990

Mol Cell Biochem

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The two clonal murine muscle cell lines G7 and G8, originally derived from the M114 line, represent unique models for comparative studies of myogenesis. Glycolipid synthesis was examined during differentiation using [3H]-galactose and [3H]-glucosamine as precursors. Upon G7 contact glucosylceramide labeling increased and nLcOse5Cer labeling stopped. During membrane fusion, glucosylceramide labeling stopped and lactosylceramide became the major synthetic product. G8 cells presented a different pattern, with increased labeling of GbOse3Cer during myogenesis. The major ganglioside synthesized by both myoblasts was GM3, and more complex structures were observed following completion of myotube formation. Total glycopeptide labeling increased when G8 myoblasts fused and remained elevated in myotubes, whereas no differences during fusion of G7 cells were noted. Upon comparison of the two clonal lines, the only consistent observation was a significant increase in the synthesis of total gangliosides and neutral glycolipid during cell contact and membrane fusion (p less than 0.02). The results suggest that changes in the synthesis of specific glycolipid structures during myogenesis are unique to each muscle cell line examined. However, transient increases in synthesis of total myoblast gangliosides and neutral glycolipids may be a more general phenomenon, possibly by curbing proliferation or by altering myoblast membrane fluidity characteristics during differentiation.

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Glycosphingolipids of an alveolar rhabdomyosarcoma and normal human muscle

Leskawa

Buse

Hogan

et al. 1984

Neurochemical Pathology

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The distribution of neutral, or asialosyl-, glycosphingolipids and gangliosides in a rhabdomyosarcoma of alveolar type have been studied. Histologically, this muscle tumor is composed primarily of two cell types: one with oval or round hyperchromatic nuclei and very little cytoplasm, and one a giant cell, with multiple, peripherally placed nuclei and weakly staining eosinophillic cytoplasm. In comparing glycolipids of the rhabdomyosarcoma with normal muscle from the same leg, the striking alteration in the tumor was a virtual disappearance of ganglioside GM2. There was also a slight increase in GM3 and a decrease in GD1a. The asialosyl derivative of GM2 (GalNac-Gal-Glc-Cer) was markedly increased in the tumor. A loss of glucosylceramide was also observed. The results are discussed in terms of glycolipid metabolic changes in muscle oncogenesis and their implications.

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Properties of endogenous, membrane-associated sialidase activity (N-acetylneuraminidase) of the goldfish visual system

Cited by 5 publications

References 47 publications

Functional Characterization of Fish Sialidases and Their Diversity among Different Orders

Functional Characterization of Fish Sialidases and Their Diversity among Different Orders

Regulation of glycolipid synthesis during differentiation of clonal murine muscle cells

Glycosphingolipids of an alveolar rhabdomyosarcoma and normal human muscle

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