Regulation of glycolipid synthesis during differentiation of clonal murine muscle cells

Leskawa, Kenneth C.; Hogan, Edward L.

doi:10.1007/bf00420908

Cited by 14 publications

(10 citation statements)

References 63 publications

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“…In contrast, very little is known about the role of glycosphingolipids in myogenesis. Pioneering studies indeed showed that myoblast differentiation was paralleled by increased biosynthesis of lactosylceramide and ganglioside GM3 [56,57]. More recently, cytosolic sialidase Neu2 was reported to be upregulated during C2C12 myoblast differentiation and, importantly, its overexpression was found to enhance myogenesis [58].…”

Section: Overview Of Sphingolipid Metabolismmentioning

confidence: 95%

Pleiotropic effects of sphingolipids in skeletal muscle

Bruni¹,

Donati²

2008

Cell. Mol. Life Sci.

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Studies of the last two decades have demonstrated that sphingolipids are important signalling molecules exerting key roles in the control of fundamental biological processes including proliferation, differentiation, motility and survival. Here we review the role of bioactive sphingolipids such as ceramide, sphingosine, sphingosine 1-phosphate, ganglioside GM3, in the regulation of skeletal muscle biology. The emerging picture is in favour of a complex role of these molecules, which appear implicated in the activation of muscle resident stem cells, their proliferation and differentiation, finalized at skeletal muscle regeneration. Moreover, they are involved in the regulation of contractile properties, tissue responsiveness to insulin and muscle fiber trophism. Hopefully, this article will provide a framework for future investigation into the field, aimed at establishing whether altered sphingolipid metabolism is implicated in the onset of skeletal muscle diseases and identifying new pharmacological targets for the therapy of multiple illnesses, including muscular dystrophies and diabetes.

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Section: Overview Of Sphingolipid Metabolismmentioning

confidence: 95%

Pleiotropic effects of sphingolipids in skeletal muscle

Bruni¹,

Donati²

2008

Cell. Mol. Life Sci.

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“…In vitro liposome-reconstitution studies suggest a different functional role for gangliosides as modulators of cytosolic calcium levels in muscle (93). Glucosphingolipid biosynthesis is tightly regulated during myogenesis in vitro, suggesting a role for membrane gangliosides in muscle cell differentiation (6,75). One of the better-understood functions of glycosphingolipids is in regulation of insulin response of muscle.…”

Section: Synthesis Of Complex Sphingolipids and Glycosphingolipidsmentioning

confidence: 99%

Sphingolipid Metabolism, Oxidant Signaling, and Contractile Function of Skeletal Muscle

Nikolova‐Karakashian

Reid

2011

Antioxidants & Redox Signaling

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Significance: Sphingolipids are a class of bioactive lipids that regulate diverse cell functions. Ceramide, sphingosine, and sphingosine-1-phosphate accumulate in tissues such as liver, brain, and lung under conditions of cellular stress, including oxidative stress. The activity of some sphingolipid metabolizing enzymes, chiefly the sphingomyelinases, is stimulated during inflammation and in response to oxidative stress. Ceramide, the sphingomyelinase product, as well as the ceramide metabolite, sphingosine-1-phosphate, can induce the generation of more reactive oxygen species, propagating further inflammation. Recent Advances: This review article summarizes information on sphingolipid biochemistry and signaling pertinent to skeletal muscle and describes the potential influence of sphingolipids on contractile function. Critical Issues: It encompasses topics related to (1) the pathways for complex sphingolipid biosynthesis and degradation, emphasizing sphingolipid regulation in various muscle fiber types and subcellular compartments; (2) the emerging evidence that implicates ceramide, sphingosine, and sphingosine-1-phosphate as regulators of muscle oxidant activity, and (3) sphingolipid effects on contractile function and fatigue. Future Directions: We propose that prolonged inflammatory conditions alter ceramide, sphingosine, and sphingosine-1-phosphate levels in skeletal muscle and that these changes promote the weakness, premature fatigue, and cachexia that plague individuals with heart failure, cancer, diabetes, and other chronic inflammatory diseases. Antioxid. Redox Signal. 15, 2501-2517.

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“…Each cell line displayed characteristic changes in the synthesis of specific GSL structures, but the exact nature of these changes were unique to each myogenic line. Only a transient increase in total neutral GSL synthesis was common to both [26].…”

Section: Discussionmentioning

confidence: 94%

“…We have recently suggested that these discrepancies may be due to the different species of origin of the cells examined because skeletal muscle GSLs exhibit a marked species specificity [24,25]. Several studies from our laboratory have consistently reported a transient increase in the synthesis of total neutral GSLs, or gangliosides, or both during in vitro myogenesis [24][25][26]. This increased synthesis was shown to coincide with myoblast fusion and was greatly exaggerated during differentiation of dystrophic muscle cells [25].…”

Section: Introductionmentioning

confidence: 98%

Glycosphingolipids during skeletal muscle cell differentiation: Comparison of normal and fusion-defective myoblasts

Cambron

Leskawa

1994

Mol Cell Biochem

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The regulation of glycosphingolipid (GSL) synthesis in culture by fusion-competent (E63) myoblasts and fusion-defective (fu-1) cells was examined. Upon reaching confluency E63 cells fused to form multinucleated myotubes and demonstrated many characteristics of developing skeletal muscle including induction of creatine kinase activity and a shift in creatine kinase isozymes to the MM isoform. The fu-1 cells displayed none of these characteristics, despite the fact that both cells were cloned from the same parental myoblast line (rat L8). There was a transient increase in the synthesis of total neutral GSLs by E63 cells at the time of membrane fusion. In contrast, neutral GSL synthesis by fu-1 cells gradually decreased with time in culture. The major GSLs synthesized by both cell types were lactosylceramide and ganglioside GM3, with more complex structures being observed with prolonged time in culture. Several glycosyltransferase activities were assayed at varying times in culture. Generally, the changes in activities fell into three groups. One group was maximally activated at the end of the culture period (GalT-3, GalNAcT-1 and GalT-6). Another group was maximally activated during the time of active membrane fusion (GlcT and SAT-1). A third group was maximally activated at the time of cell contact and the beginning of membrane fusion (GlcNAcT-1 and GalT-2). In terms of the times of maximal activation there were few differences between E63 and fu-1 cells, with one notable exception. The activity of GalT-2 (lactosylceramide synthase) in E63 cells increased dramatically upon contact and the beginning of membrane fusion, whereas there were no changes in GalT-2 activity in fu-1 cells during time in culture. These results support our hypothesis that membrane glycosphingolipids play an important role in the differentiation of skeletal muscle cells.

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Regulation of glycolipid synthesis during differentiation of clonal murine muscle cells

Cited by 14 publications

References 63 publications

Pleiotropic effects of sphingolipids in skeletal muscle

Pleiotropic effects of sphingolipids in skeletal muscle

Sphingolipid Metabolism, Oxidant Signaling, and Contractile Function of Skeletal Muscle

Glycosphingolipids during skeletal muscle cell differentiation: Comparison of normal and fusion-defective myoblasts

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