Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media

Fahrbach, Florian O.; Rohrbach, Alexander

doi:10.1038/ncomms1646

Cited by 284 publications

(206 citation statements)

References 19 publications

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“…Finally, alternative illumination schemes other than the Gaussian beams modeled within this study have also been reported in the literature (structured illumination, Bessel and Airy beams, multi-photon, etc.) [49][50][51][52], which may be explored using similar methodologies in future studies.…”

Section: Discussionmentioning

confidence: 99%

Assessing the imaging performance of light sheet microscopies in highly scattering tissues

Glaser

Wang

Liu

2016

Biomed. Opt. Express

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Light sheet microscopy (LSM) has emerged as an opticalimaging method for high spatiotemporal volumetric imaging of relatively transparent samples. While this capability has allowed the technique to be highly impactful in fields such as developmental biology, applications involving highly scattering thick tissues have been largely unexplored. Herein, we employ Monte Carlo simulations to explore the use of LSM for imaging turbid media. In particular, due to its similarity to dual-axis confocal (DAC) microscopy, we compare LSM performance to pointscanned (PS-DAC) and line-scanned (LS-DAC) dual-axis confocal microscopy techniques that have been previously shown to produce highquality images at round-trip optical lengths of ~9 -10 and ~3 -4 respectively. The results of this study indicate that LSM using widefield collection (WF-LSM) provides comparable performance to LS-DAC in thick tissues, due to the fact that they both utilize an illumination beam focused in one dimension (i.e. a line or sheet). On the other hand, LSM using confocal line detection (CL-LSM) is more analogous to PS-DAC microscopy, in which the illumination beam is focused in two dimensions to a point. The imaging depth of LSM is only slightly inferior to DAC (~2 -3 and ~6 -7 optical lengths for WF-LSM and CL-LSM respectively) due to the use of a lower numerical aperture (NA) illumination beam for extended imaging along the illumination axis. Therefore, we conclude that the ability to image deeply is dictated most by the confocality of the microscope technique. In addition, we find that imaging resolution is mostly dependent on the collection NA, and is relatively invariant to imaging depth in a homogeneous scattering medium. Our results indicate that superficial imaging of highly scattering tissues using light sheet microscopy is possible.

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Section: Discussionmentioning

confidence: 99%

Assessing the imaging performance of light sheet microscopies in highly scattering tissues

Glaser

Wang

Liu

2016

Biomed. Opt. Express

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“…Once, it was shown that they are "selfreconstructing" [12] and show enhanced penetration into scattering media [13], they are increasingly investigated in microscopy [14]. Currently, they are explored for their application in neurosciences for opto-genetic stimulation.…”

Section: Discussionmentioning

confidence: 99%

Adaptive micro axicons for laser applications

Wallrabe

Brunne

Treffer

et al. 2015

MATEC Web of Conferences

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Abstract. We report on the design, fabrication and testing of novel types of low-dispersion axicons for the adaptive shaping of ultrashort laser pulses. An overview is given on the basic geometries and operating principles of our purely reflective adaptive MEMS-type devices based on thermal or piezoelectric actuation. The flexible formation of nondiffracting beams at pulse durations down to a few oscillations of the optical field enables new applications in optical communication, pulse diagnostics, laser-matter interaction and particle manipulation. As an example, we show first promising results of adaptive autocorrelation. The combination of excellent pulse transfer, self-reconstruction properties and propagation invariance of nondiffracting beams with an adaptive approach promises to extend the field of practical applications significantly.

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“…39 To exploit the detector's full dynamic range, line confocal detection coupled with scanning illumination has been proposed. 40,41 In the scheme described by Fahrbach and Rohrbach, confocal detection is achieved by exposing the camera at each position of the excitation line and subsequently retaining only the pixel line confocal to the excitation beam. 40 Although this confocal approach allows a signi¯cant enhancement of image contrast, the camera needs to be exposed and readout at each beam position to produce a single¯nal image of the whole¯eld of view, and background rejection is achieved by post-processing the acquired data.…”

Section: Whole-brain High-resolution Neuroanatomymentioning

confidence: 99%

Advanced Optical Techniques to Explore Brain Structure and Function

Silvestri

Mascaro

Lotti

et al. 2013

J. Innov. Opt. Health Sci.

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Understanding brain structure and function, and the complex relationships between them, is one of the grand challenges of contemporary sciences. Thanks to their°exibility, optical techniques could be the key to explore this complex network. In this manuscript, we brie°y review recent advancements in optical methods applied to three main issues: anatomy, plasticity and functionality. We describe novel implementations of light-sheet microscopy to resolve neuronal anatomy in whole¯xed brains with cellular resolution. Moving to living samples, we show how real-time dynamics of brain rewiring can be visualized through two-photon microscopy with the spatial resolution of single synaptic contacts. The plasticity of the injured brain can also be dissected through cutting-edge optical methods that speci¯cally ablate single neuronal processes. Finally, we report how nonlinear microscopy in combination with novel voltage sensitive dyes allow optical registrations of action potential across a population of neurons opening promising prospective in understanding brain functionality. The knowledge acquired from these complementary optical methods may provide a deeper comprehension of the brain and of its unique features.

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Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media

Cited by 284 publications

References 19 publications

Assessing the imaging performance of light sheet microscopies in highly scattering tissues

Assessing the imaging performance of light sheet microscopies in highly scattering tissues

Adaptive micro axicons for laser applications

Advanced Optical Techniques to Explore Brain Structure and Function

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