Proliferative activity in oral salivary gland tumors: the role of PCNA and AgNOR assessed by a double staining technique

Rivero, Elena Riet Correa; Caliari, Marcelo Vidigal; Tarquínio, Sandra Beatriz Chaves; Loyola, Adriano Mota; Aguiar, Maria Cássia Ferreira de

doi:10.2334/josnusd.46.87

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…The sections were dewaxed in xylene and hydrated through decreasing grades of ethanol. The silver staining was applied according to the method of Ploton et al [ 10 ] modified by Rivero et al [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of AgNORs in Oral Potentially Malignant Lesions

Tomazelli

Modolo

Rivero

2015

Journal of Oncology

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Oral squamous cell carcinoma (OSCC) is usually preceded by detectable mucosal changes, as leukoplakias and erythroplakia. Histologically, these lesions can range from hyperkeratosis and acanthosis to epithelial dysplasia and even OSCC. The aim of this study was to investigate the proliferative activity, using AgNORs quantification proteins, in low- and high-risk oral epithelial dysplasia, OSCC, and nondysplastic epithelium (inflammatory fibrous hyperplasia). The sample was divided into 4 groups: G1: 10 cases of inflammatory fibrous hyperplasia (IFH), G2: 11 cases of low-risk epithelial dysplasia (LD), G3: 10 cases of high-risk epithelial dysplasia (HD), and G4: 11 cases of OSCC. The quantitative analysis was performed using an image processing software in photomicrographs at 1000x magnification. The one-way ANOVA was used for comparison of the mean AgNORs counts between the study groups. The mean AgNORs count was significantly higher (P ≤ 0.01) in OSCC when compared to IFH and the LD; however, it was not statistically different from HD. The mean number of LD was significantly lower than the HD and OSCC, with no difference related to IFH. AgNORs quantification can be an important and cheap method to help in the determination of the degree of epithelial dysplasia and, consequently, in the analysis of their potential for malignant transformation.

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Section: Methodsmentioning

confidence: 99%

Evaluation of AgNORs in Oral Potentially Malignant Lesions

Tomazelli

Modolo

Rivero

2015

Journal of Oncology

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“…Higher expression of this marker has been shown in more aggressive tumours [23]. For the evaluation of salivary gland cancer, PCNA appears to have predictive significance, although some conflicting results have been reported in studies of small group numbers of different histological subtypes [17,24,25]. Against this background, PCNA seems to be one of the few hopeful prognostic markers.…”

Section: Introductionmentioning

confidence: 95%

Prognostic value of proliferating cell nuclear antigen in parotid gland cancer

Stenner

Demgensky

Molls

et al. 2011

Eur Arch Otorhinolaryngol

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Although cell proliferation is related to tumour aggressiveness and prognosis, there are few studies describing the expression of proliferative markers in salivary gland cancer. Our aim was to assess the long-term prognostic value of the proliferating cell nuclear antigen (PCNA) in a large group of histologically different salivary gland cancers. We analysed the expression of PCNA in 159 patients with parotid gland cancer by means of immunohistochemistry. The mean follow-up time was 56.6 months. A high expression of PCNA showed a significant correlation to the patients' pathological lymph node stage (p = 0.004). A high PCNA expression significantly indicated a poor 5-year disease-free (p = 0.046) and overall survival rate (p = 0.018). The PCNA expression was the only prognostic factor for a worse 5-year disease-free and overall survival in acinic cell carcinomas (p = 0.004, p = 0.022). The correlation between PCNA expression and survival probabilities of salivary gland cancer might make proliferation markers helpful tools in patient follow-up, prognosis and targeted therapy in salivary gland cancer in future.

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Proliferative Activity in Oral Carcinomas Studied with Ag-NOR and Electron Microscopy

Kummoona

Jabbar

Al-Rahal

2008

Ultrastructural Pathology

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This study was conducted on 61 patients (27 males and 34 females). The age ranged from 27 to 81 years (mean 54 years). All suffered from oral squamous cell carcinoma and were treated by surgery and deep X-ray therapy (DXT). The UICC and TNM classification and staging recommendations were used for evaluation of the patients. All sections were stained with Ag-NORs stain for examination of the proliferative activity of the squamous cell carcinomas. Biopsies were also taken from another 6 cases--3 cases with normal striated muscle and 3 cases from normal oral mucosa--and served as controls. Statistical studies of Ag-NOR scores were classified into 3 scores: the p values of score I (ANOVA test) were .0001, score II (ANOVA test) was .0001, and score III (ANOVA test) was 06. Both scores I and II were highly significant and score III was significant. Electron microscopy (EM) showed tumor cells with irregular shape and size and with remarkable division of nuclei and chromatin clumps emarginated toward nuclear membrane, and some cases showed chromatin condensed at one pole of the nucleus. Few mitochondria with dilated cristae (?) and abundant rough endoplasmic reticulum (RER) were observed. Few apoptotic changes were noted. This study showed a high proliferation in poorly differentiated squamous cell carcinomas. The amount of Ag-NOR in poorly differentiated squamous cell carcinomas was a prognostic factor and represented an unfavorable prognostic feature in squamous cell carcinoma of the oral mucosa.

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Proliferative activity in oral salivary gland tumors: the role of PCNA and AgNOR assessed by a double staining technique

Cited by 4 publications

References 44 publications

Evaluation of AgNORs in Oral Potentially Malignant Lesions

Evaluation of AgNORs in Oral Potentially Malignant Lesions

Prognostic value of proliferating cell nuclear antigen in parotid gland cancer

Proliferative Activity in Oral Carcinomas Studied with Ag-NOR and Electron Microscopy

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