Proliferation in the normal cervix and in preinvasive cervical lesions.

Abstract

Aims-To characterise further the proliferative compartment of the normal cervix and to document its alteration, if any, in the various grades of cervical intraepithelial neoplasia (CIN), particularly changes to the basal epithelial layer; to hypothesise as to the diagnostic and biological significance of any observed differences. (7 Clin Pathol 1996;49:667-671)

Role of apoptotic index, mitotic index and MIB-1 antibody expression as biomarkers in preneoplastic and neoplastic lesions of uterine cervix

“…14 In the present study, it was observed that MIB-1 positive cells were confined to lower one-third of ectocervix in CIN-1 and full thickness in CIN-III. This was in concordance with the study conducted by McCluggage WG et al, Payne S et al and Sansanwal P et al [23][24][25] Payne P et al observed that with increasing grade of CIN, the growth compartment stretched more superficially so that in CIN III almost the full thickness of epithelium was cycling, as shown by Ki-67 immuno-positive cells. 25 In present study, we observed increasing MIB-1 positivity while moving through different severity of CINs, difference being statistically very significant.…”

“…This was in concordance with the study conducted by McCluggage WG et al, Payne S et al and Sansanwal P et al [23][24][25] Payne P et al observed that with increasing grade of CIN, the growth compartment stretched more superficially so that in CIN III almost the full thickness of epithelium was cycling, as shown by Ki-67 immuno-positive cells. 25 In present study, we observed increasing MIB-1 positivity while moving through different severity of CINs, difference being statistically very significant. This is in concordance with the study of Payne P et al, Bulten J et al, ter Harmsel B et al, Nam JH et al, Conesa-Zamora P et al, Gupta K et al and Sansanwal P et al (Table 6).…”

“…The studies conducted by Nam JH et al, Pahuja S et al and Sansanwal P et al also showed an increase in LI from WD-SCC to PD-SCC, similar to present study. 19,23,[25][26][27] In contrast, Gupta K et al found the mean LI of MD-SCC to be less than WD-SCC, while it was maximum in PD-SCC like our study.…”

“…This is in concordance with the study of Payne P et al, Bulten J et al, ter Harmsel B et al, Nam JH et al, Conesa-Zamora P et al, Gupta K et al and Sansanwal P et al (Table 6). [23][24][25][26][27][28][29] We also observed that the mean value of MIB-1 Labelling index increased significantly as the nature of the lesion changed from dysplasia to SCC. Similar results were observed by Nam JH et al, Mehrotra A et al, Srivastava S et al and Gupta K et al 19,15,29,21 We also found an increasing trend in MIB-1 Labelling index (LI) from WD-SCC to PD-SCC.…”

Activation of the Enhancer of Zeste Homologue 2 Gene by the Human Papillomavirus E7 Oncoprotein

“…However, there was no obvious correlation between EZH2 protein levels and the degree of malignancy of the lesions. Whereas the regular detection of high EZH2 protein levels in HPV-positive lesions is fully compatible with EZH2 being a target for oncogenic HPVs, it is notable that we also clearly detected EZH2 expression in the parabasal cells of the cervical mucosa, which represents the main proliferative cell layer in normal cervix (42). Thus, EZH2 expression is unlikely to serve as a novel tumor marker for cervical cancer, in contrast to the situation reported for breast cancer where EZH2 expression is elevated in precancers and cancers but nearly undetectable in normal breast samples (43).…”