Proinflammation in maternal and fetal livers and circulating miR-122 dysregulation in a GDM rat model induced by intrauterine programming

Fornes, Daiana; Heinecke, Florencia; Roberti, Sabrina Lorena; White, Verónica; Capobianco, Evangelina; Jawerbaum, Alicia

doi:10.1016/j.mce.2020.110824

Cited by 13 publications

(12 citation statements)

References 49 publications

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“…In contradiction to our study, Carreras-Badosa et al found that maternal pre-gestational obesity and gestational weight gain were associated with decreased levels of second trimester (24-32 weeks of gestation) plasma miR-122-5p [36]. Moreover, a recent study on intrauterine fetal programming and GDM showed decreased circulating maternal and fetal miR-122-5p in a GDM rat model [84]. The reason for these discrepancies is not known, but could be due to differences in ethnicity of patients and interspecies differences in miRNA regulation.…”

Section: Discussioncontrasting

confidence: 99%

The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Sørensen

Poppel

Desoyé

et al. 2021

Cells

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Early identification of gestational diabetes mellitus (GDM) aims to reduce the risk of adverse maternal and perinatal outcomes. Currently, no circulating biomarker has proven clinically useful for accurate prediction of GDM. In this study, we tested if a panel of small non-coding circulating RNAs could improve early prediction of GDM. We performed a nested case-control study of participants from the European multicenter ‘Vitamin D and lifestyle intervention for GDM prevention (DALI)’ trial using serum samples from obese pregnant women (BMI ≥ 29 kg/m2) entailing 82 GDM cases (early- and late- GDM), and 41 age- and BMI-matched women with normal glucose tolerance (NGT) throughout pregnancy (controls). Anthropometric, clinical and biochemical characteristics were obtained at baseline (<20 weeks of gestation) and throughout gestation. Baseline serum microRNAs (miRNAs) were measured using quantitative real time PCR (qPCR). Elevated miR-16-5p, -29a-3p, and -134-5p levels were observed in women, who were NGT at baseline and later developed GDM, compared with controls who remained NGT. A combination of the three miRNAs could distinguish later GDM from NGT cases (AUC 0.717, p = 0.001, compared with fasting plasma glucose (AUC 0.687, p = 0.004)) as evaluated by area under the curves (AUCs) using Receiver Operator Characteristics (ROC) analysis. Elevated levels of individual miRNAs or a combination hereof were associated with higher odds ratios of GDM. Conclusively, circulating miRNAs early in pregnancy could serve as valuable predictive biomarkers of GDM.

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Section: Discussioncontrasting

confidence: 99%

The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Sørensen

Poppel

Desoyé

et al. 2021

Cells

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“…MiRNA could be found in the liver and blood plasma, especially in the liver. 30,31 The method was applied to the determination of miR-122 in the livers of chickens and ducks. The extracted RNA solutions were tested and the typical results are shown in Fig.…”

Section: Determination Of Mir-122 In Livermentioning

confidence: 99%

Determination of microRNA-122 in hepatocytes by two-step amplification of duplex-specific nuclease with laser-induced fluorescence detection

Zhang

Wang

2022

Anal. Methods

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“…With the downregulation of miR-873 in GDM rats, heart function was improved and myocardial apoptosis was inhibited ( 96 ). In addition, the expression of miRNA-122 was downregulated in the plasma of GDM rats, and miR-122 levels were regulated both in vitro through PPARγ activation and in vivo through a maternal diet enriched in PPAR ligands ( 87 ). Furthermore, the miRNA-221 level in the placental tissues of GDM rats was down-regulated compared with the control group; the overexpression of miRNA-221 stimulated insulin secretion, cell proliferation, and suppressed apoptosis via PAK1 in INS-1 cells ( 93 ).…”

Section: Role Of Non-coding Rnas and Evs In Gdmmentioning

confidence: 99%

Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

et al. 2021

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Gestational diabetes mellitus (GDM) is defined as glucose intolerance with onset or first recognition in the second or third trimester of pregnancy. GDM has a considerable impact on health outcomes of the mother and offspring during pregnancy, delivery, and beyond. Although the exact mechanism regarding GDM remains unclear, numerous studies have suggested that non-coding RNAs, including long non-coding (lnc)RNAs, microRNAs, and circular RNAs, were involved in the pathogenesis of GDM in which they played vital regulatory roles. Additionally, several studies have revealed that extracellular vehicles also participated in the pathogenesis of GDM, highlighting their important role in this disease. Considering the lack of effective biomarkers for the early identification of and specific treatment for GDM, non-coding RNAs and extracellular vehicles may be promising biomarkers and even targets for GDM therapies. This review provides an update on our understanding of the role of non-coding RNAs and extracellular vehicles in GDM. As our understanding of the function of lncRNAs and extracellular vehicles improves, the future appears promising for their use as potential biomarkers and treatment targets for GDM in clinical practice.

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Proinflammation in maternal and fetal livers and circulating miR-122 dysregulation in a GDM rat model induced by intrauterine programming

Cited by 13 publications

References 49 publications

The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Determination of microRNA-122 in hepatocytes by two-step amplification of duplex-specific nuclease with laser-induced fluorescence detection

Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

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