Determination of microRNA-122 in hepatocytes by two-step amplification of duplex-specific nuclease with laser-induced fluorescence detection

Abstract

MicroRNAs (miRNAs) play important roles in physiological and pathological processes of cells. To develop a fast, simple and sensitive method to determine miRNAs is significant for miRNA studies. In this...

A highly sensitive fluorescence sensor for tobacco mosaic virus RNA based on DSN cycle and ARGET ATRP double signal amplification

“…In the DSN target cycle strategy, the DSN selectively cleaves DNA from doublestranded DNA or DNA-RNA hybrids without affecting singlestranded DNA or double-stranded RNA, which could distinguish between perfect matches and full mismatches of short double genes [16][17][18]. Therefore, TMV RNA (tRNA) is used to open the hairpin DNA to form a double-stranded structure, and then, DSN catalyzes the double-stranded hydrolysis to release tRNA; tRNA continues to open other hairpin DNA, so that more hairpin DNA can be opened through a small amount of tRNA cycle [19,20]. For example, Ashraf et al [17] constructed a highly sensitive RNA detection sensor designed with RNA ligand sequences for target capture and DSNassisted fluorescence signal enhancement with a detection limit of 39 fM.…”