The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Sørensen, Anja Elaine; Poppel, Mireille N. M. van; Desoyé, Gernot; Damm, Peter; Simmons, David; Jensen, Dorte Møller; Dalgaard, Louise Torp

doi:10.3390/cells10010170

Cited by 37 publications

(89 citation statements)

References 96 publications

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“…Another study revealed increased expression of miR16-5p in overweight women before the 20 th week of pregnancy. In contrast to previously cited reports, that study was conducted on European women (34). On the other hand, Martinez-Ibarra et al demonstrated no significant changes in miR16-5p expression in serum collected in the 2 nd trimester from GDM patients compared to NGT (35).…”

Section: Discussionmentioning

confidence: 59%

miRNAs as Predictive Factors in Early Diagnosis of Gestational Diabetes Mellitus

et al. 2022

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IntroductionCirculating miRNAs are important mediators in epigenetic changes. These non-coding molecules regulate post-transcriptional gene expression by binding to mRNA. As a result, they influence the development of many diseases, such as gestational diabetes mellitus (GDM). Therefore, this study investigates the changes in the miRNA profile in GDM patients before hyperglycemia appears.Materials and MethodsThe study group consisted of 24 patients with GDM, and the control group was 24 normoglycemic pregnant women who were matched for body mass index (BMI), age, and gestational age. GDM was diagnosed with an oral glucose tolerance test between the 24th and 26th weeks of pregnancy. The study had a prospective design, and serum for analysis was obtained in the first trimester of pregnancy. Circulating miRNAs were measured using the NanoString quantitative assay platform. Validation with real time-polymerase chain reaction (RT-PCR) was performed on the same group of patients. Mann-Whitney U-test and Spearman correlation were done to assess the significance of the results.ResultsAmong the 800 miRNAs, 221 miRNAs were not detected, and 439 were close to background noise. The remaining miRNAs were carefully investigated for their average counts, fold changes, p-values, and false discovery rate (FDR) scores. We selected four miRNAs for further validation: miR-16-5p, miR-142-3p, miR-144-3p, and miR-320e, which showed the most prominent changes between the studied groups. The validation showed up-regulation of miR-16-5p (p<0.0001), miR-142-3p (p=0.001), and miR-144-3p (p=0.003).ConclusionWe present changes in miRNA profile in the serum of GDM women, which may indicate significance in the pathophysiology of GDM. These findings emphasize the role of miRNAs as a predictive factor that could potentially be useful in early diagnosis.

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Section: Discussionmentioning

confidence: 59%

miRNAs as Predictive Factors in Early Diagnosis of Gestational Diabetes Mellitus

et al. 2022

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“…We revealed that leptin was one of the molecular mechanisms underlying miR-29a control of systemic adiposity as HFD-induced leptin signaling was compromised in miR-29aTg bone. Serum or exosomal miR-29a involves the development of obesity or HFD-induced diabetes [53,54]. Other groups and we have revealed that low serum miR-29-3p or miR-29a levels are present in aged patients with osteoporosis [55] or postmenopausal women with osteoporotic spine fracture [56].…”

Section: Discussionmentioning

confidence: 69%

MicroRNA-29a in Osteoblasts Represses High-Fat Diet-Mediated Osteoporosis and Body Adiposis through Targeting Leptin

Lian

Chen

et al. 2021

IJMS

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Skeletal tissue involves systemic adipose tissue metabolism and energy expenditure. MicroRNA signaling controls high-fat diet (HFD)-induced bone and fat homeostasis dysregulation remains uncertain. This study revealed that transgenic overexpression of miR-29a under control of osteocalcin promoter in osteoblasts (miR-29aTg) attenuated HFD-mediated body overweight, hyperglycemia, and hypercholesterolemia. HFD-fed miR-29aTg mice showed less bone mass loss, fatty marrow, and visceral fat mass together with increased subscapular brown fat mass than HFD-fed wild-type mice. HFD-induced O2 underconsumption, respiratory quotient repression, and heat underproduction were attenuated in miR-29aTg mice. In vitro, miR-29a overexpression repressed transcriptomic landscapes of the adipocytokine signaling pathway, fatty acid metabolism, and lipid transport, etc., of bone marrow mesenchymal progenitor cells. Forced miR-29a expression promoted osteogenic differentiation but inhibited adipocyte formation. miR-29a signaling promoted brown/beige adipocyte markers Ucp-1, Pgc-1α, P2rx5, and Pat2 expression and inhibited white adipocyte markers Tcf21 and Hoxc9 expression. The microRNA also reduced peroxisome formation and leptin expression during adipocyte formation and downregulated HFD-induced leptin expression in bone tissue. Taken together, miR-29a controlled leptin signaling and brown/beige adipocyte formation of osteogenic progenitor cells to preserve bone anabolism, which reversed HFD-induced energy underutilization and visceral fat overproduction. This study sheds light on a new molecular mechanism by which bone integrity counteracts HFD-induced whole-body fat overproduction.

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“… 158CTRL Li Deng 21 68GDM 24–28 miR-29a/b – Human Serum Neonatal pathologic jaundice 55CTRL Songbo Fu 22 90 GDM – miR-875-5p TXNRD1 Rat Serum Reduced FBG and insulin resistance, reduced expression levels of blood lipid and pro-inflammatory markers as well as reduced oxidative stress 10CTRL Guido Sebastiani 23 21 GDM 24–33 miR-330-3p Directly be transferred from plasma to beta-cells Human Plasma Modulating key target genes involved in proliferation, differentiation, and insulin secretion. 10 CTRL Jianping Wang 24 102 GDM 24–28 miR-195-5p – Human Serum Fasting plasma glucose, one‐hour plasma glucose, two‐hour plasma glucose, and BMI 102 CRTL Sujuan Dai 25 67GDM 24–28 miR-2467 Adiponectin Human Serum Increase (BMI), TC, TG, LDL-C, FPG, HbA1c, HOMA-IR 60CTRL Anja Elaine Sørensen 26 82GDM <20,24–28,35-37 miR-16-5p, −29a-3p, and −134-5p – Human ...…”

Section: Mirnas and Gdm Pathophysiologymentioning

confidence: 99%

“…The combined application three miRNAs is more suitable than fasting blood glucose in distinguishing advanced GDM and NGT cases, and both miR-16-5p and miR-29a-3p are related to macrosomia. 26 The up-regulated level of serum miR-1323 can be used as a diagnostic biomarker for GDM disease. Furthermore, under the condition of HG, knocking out miR-1323 in trophoblast cells may promote trophoblast cells survival by targeting TP53INP1.…”

Section: Mirnas and Gdm Pathophysiologymentioning

confidence: 99%

MicroRNAs and Exosomal microRNAs May Be Possible Targets to Investigate in Gestational Diabetes Mellitus

Yang¹,

Wu²

2022

DMSO

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Gestational diabetes mellitus (GDM) is defined as glucose intolerance that occurs during the second or third trimester of pregnancy. As the incidence of GDM rises, so does the risk of maternal and fetal complications with short- and long-term consequences. As a result, early diagnosis and treatment of this condition are important to avoiding adverse pregnancy outcomes. Exosomes are tiny vesicles secreted by living cells which contain a variety of bioactive substances. They are released by cells to facilitate cell-to-cell communication and regulate a variety of biological processes such as cellular immune response, inflammatory response, and apoptosis, among others. Many studies have recently confirmed that changes in the expression and secretion of exosomal miRNAs can be used as novel markers for the diagnosis, prognosis, and treatment of GDM. In this review, we summarized the various roles of exosomal miRNAs and circulating miRNAs in GDM. We found that the changes in the expression of certain miRNAs could be used to diagnosing GDM. Exosomal miRNAs target metabolic pathways, resulting in insulin resistance. We also highlighted the potential for miRNAs and exosomal miRNAs to be used as biomarkers for diagnosis or therapeutic agents.

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The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Cited by 37 publications

References 96 publications

miRNAs as Predictive Factors in Early Diagnosis of Gestational Diabetes Mellitus

miRNAs as Predictive Factors in Early Diagnosis of Gestational Diabetes Mellitus

MicroRNA-29a in Osteoblasts Represses High-Fat Diet-Mediated Osteoporosis and Body Adiposis through Targeting Leptin

MicroRNAs and Exosomal microRNAs May Be Possible Targets to Investigate in Gestational Diabetes Mellitus

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