Progress in immunoassays for nitrofurans detection

Wu, Weilin; Yang, Shuyu; Liu, Jialiang; Mi, Jiafei; Dou, Leina; Pan, Yantong; Mari, Ghulam Mujtaba; Wang, Zhanhui

doi:10.1080/09540105.2020.1786672

Cited by 23 publications

(5 citation statements)

References 40 publications

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“…Wu et al et al ( Wu et al, 2020 ) gave a detailed introduction to ELISA studies to detect AOZ, SEM, AHD, and AMOZ before 2020. Yan et al ( Yan et al, 2020b ) combined Au NPs into conventional ELISA for sensitive detection of AMOZ, thereby improving detection sensitivity.…”

Section: Research Progress Of Immunosensorsmentioning

confidence: 99%

Immunosensor of Nitrofuran Antibiotics and Their Metabolites in Animal-Derived Foods: A Review

Jia

Zhang

et al. 2022

Front. Chem.

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Nitrofuran antibiotics have been widely used in the prevention and treatment of animal diseases due to the bactericidal effect. However, the residual and accumulation of their metabolites in vivo can pose serious health hazards to both humans and animals. Although their usage in feeding and process of food-derived animals have been banned in many countries, their metabolic residues are still frequently detected in materials and products of animal-derived food. Many sensitive and effective detection methods have been developed to deal with the problem. In this work, we summarized various immunological methods for the detection of four nitrofuran metabolites based on different types of detection principles and signal molecules. Furthermore, the development trend of detection technology in animal-derived food is prospected.

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Section: Research Progress Of Immunosensorsmentioning

confidence: 99%

Immunosensor of Nitrofuran Antibiotics and Their Metabolites in Animal-Derived Foods: A Review

Jia

Zhang

et al. 2022

Front. Chem.

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“…Nitrofurans break down rapidly in the organism, and their metabolites bind to proteins, including those in muscle tissue, and can persist there for several weeks [ 14 , 15 ]. Nitrofurans have four persistent metabolites (NM): 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AHD), and semicarbazide (SEM) [ 16 ]. AOZ is a marker metabolite for furazolidone, AMOZ—for furaltadone, AHD—for nitrofurantoin, and SEM—for nitrofurazone [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Exposure Assessment of Nitrofuran Metabolites in Fish and Honey Produced in Armenia: A Pilot Investigation

Pipoyan,

Beglaryan,

Chirkova

et al. 2023

Foods

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In Armenia, the presence of nitrofuran residues in food products is unacceptable for both domestic sales and export. However, food may contain nitrofuran metabolites (NMs) due to the illegal use of these drugs in the agrofarming practice. This study aimed to identify NMs as the marker residues for nitrofurans in fish and honey produced in Armenia and assess the potential health risks associated with consuming these foods. The commodities studied were natural honey and three species of farmed fish produced by various regions nationwide. Concentrations of the marker metabolites (3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AHD), and semicarbazide (SEM)) were determined through an enzyme-linked immunosorbent assay (ELISA) and verified using liquid chromatography–mass spectrometry (LC-MS/MS). Consumer groups were identified based on their average daily intake of foods. Health risk was assessed by calculating the margin of exposure (MOE). Reference values for health risk assessment were obtained from the European Food Safety Authority (EFSA). Results showed that 33.3% of fish samples and 44.4% of honey samples contained NMs, the mean concentrations ranging from 0.05 μg/kg to 0.52 μg/kg. All MOE values obtained were over 10,000, indicating that the detected concentrations of NMs in fish and honey produced in Armenia pose no health risk to consumers. However, these results highlight the illicit use of highly toxic substances and the need for improved control of farming practices.

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“…Toxicology research indicated that NFs and their metabolites are potentially hazardous substances, with possible carcinogenic, teratogenic, and mutagenic properties [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Determination of Nitrofuran Metabolites in Complex Food Matrices Using a Rough, Cheap, Easy-Made Wooden-Tip-Based Solid-Phase Microextraction Probe and LC-MS/MS

Gong

et al. 2022

Journal of Chemistry

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In this study, a rough, cheap, easy-made wooden-tip-based solid-phase microextraction (SPME) probe was first developed for simultaneous determination of 4 nitrofuran metabolite derivatives in complex food matrices via LC-MS/MS. A simple dip-coating method was used to coat wooden tips with biocompatible polyacrylonitrile (PAN) and N-vinylpyrrolidone-co-divinylbenzene, also known as HLB particles, which served as the extractive substrate in the proposed device. Compared with the traditional solid-phase extraction (SPE) method, the proposed device shortens sample clean-up time, reduces solvent consumption, and decreases testing costs. In addition, the main parameters affecting the SPME procedure efficiency were investigated in detail and the optimal conditions were found. The method was validated using three different food matrixes (pork, croaker, and honey) by spiking with the four metabolites at 0.5, 1.0, and 5.0 μg/kg, as well as their internal standards. The average recovery of all nitrofuran metabolite derivatives ranges from 97.4–109.5% (pork), 87.5–112.7% (croaker), and 98.6–109.0% (honey). Relative standard deviations were all <10% for intraday and interday precision. The values of limit of detection and limit of quantification were, respectively, ranging from 0.011 to 0.123 and 0.033 to 0.369 μg/kg (pork), 0.009 to 0.112 and 0.027 to 0.339 μg/kg (croaker), and 0.010 to 0.131 and 0.030 to 0.293 μg/kg (honey). The presented method was applied to the analysis of real positive samples.

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Progress in immunoassays for nitrofurans detection

Cited by 23 publications

References 40 publications

Immunosensor of Nitrofuran Antibiotics and Their Metabolites in Animal-Derived Foods: A Review

Immunosensor of Nitrofuran Antibiotics and Their Metabolites in Animal-Derived Foods: A Review

Exposure Assessment of Nitrofuran Metabolites in Fish and Honey Produced in Armenia: A Pilot Investigation

Determination of Nitrofuran Metabolites in Complex Food Matrices Using a Rough, Cheap, Easy-Made Wooden-Tip-Based Solid-Phase Microextraction Probe and LC-MS/MS

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