Programmable Building of Radially Gradient Nanofibrous Patches Enables Deployment, Bursting Bearing Capability, and Stem Cell Recruitment

Abstract

Fibrous patches capable of withstanding bursting force and recruiting endogenous stem cells are of great demand for wound treatment. A programmable strategy for development of radially gradient nanofibrous patches with rapid deployment property, robust bursting bearing capability, and excellent mesenchymal stem cell (MSC) recruitment capability, is demonstrated. Benefiting from the royal water lily‐like radially branched architecture, the gradient fibrous (GF) patches exhibit fast deployment in aqueous solution (2 s), high bursting strength of 4.6 N, as well as “center‐to‐periphery” gradient immobilization of stromal‐cell‐derived factor 1α (SDF1α). The SDF1α gradient patches direct MSC migration from the periphery to the center along the aligned nanofibers, resulting in a 4.2 times higher migrated cell number and 2.6 times greater maximum migration distance than random fibrous patches with homogenous SDF1α. The gelatin methacryloyl coated GF patches respond to matrix metalloproteinase‐9 for “on‐demand” release of anti‐inflammatory drug diclofenac sodium (DS). Furthermore, repair of the mouse full‐thickness skin incision validates that SDF1α/DS/GF patches are able to provide feasible microenvironment to attenuate inflammation and improve endogenous MSC recruitment, leading to accelerated wound healing. This work may open a new pathway for development of smart tough fibrous patches for stimulating endogenous repair mechanisms during tissue regeneration.

One-dimensional nanomaterials for cancer therapy and diagnosis

“…In 2021, alkaline phosphatase (ALP)-responsive extracellular nanofiber self-assembly was reported, which was found to accumulate after cellular internalization, thereby interrupting intracellular networks. 18 Typically, cancer cells exhibited overexpression of ALP on their membranes, which normally regulates cell signaling. Triggered by the ALPs, densely entangled bundles of elongated nanofibers formed on the surfaces of cancer cells, thereby hindering their biomolecule exchange.…”

Electrospun nanofibers for manipulating soft tissue regeneration

“…Du et al designed a radially assembled PCL/collagen electrospun nanofiber scaffold encapsulated with the anti-inflammatory agent, diclofenac sodium. 158 A gradient of SDF-1α increasing from the periphery to the center was also constructed on the surface (Fig. 5C).…”

Radially Electrospun Fibrous Membrane Incorporated with Copper Peroxide Nanodots Capable of Self-Catalyzed Chemodynamic Therapy for Angiogenesis and Healing Acceleration of Diabetic Wounds

“…Meanover, the released Cu 2+ ions could also facilitate angiogenesis by upregulating the angio-related genes. Besides, as a topographical modulator, the radially arranged fibrous structure could also mimic the intrinsic wound healing direction and induce cells to migrate and proliferate toward the center of the membrane. − By combining the dual regulations physically and chemically, the Ran@CP achieved both pH-responsive CDT and orientated cell migration and finally accelerated wound healing and vascular regeneration for diabetic wounds.…”