Profiling of water-soluble carbohydrates in pine and spruce extracts by capillary zone electrophoresis with direct UV detection

Abstract

Monitoring wood-related biorefinery process streams is important as the composition of raw material and its variations have a great impact on the production outcome as well as on the process control. In this paper, the applicability of a simple type of capillary zone electrophoresis method to profiling heterogeneous woodbased samples was studied by grouping the peaks in the overall electropherograms. A comparison between 210 and 270 nm as an example confirmed the identification of a monosaccharide peak in the carbohydrate electropherogram. The analytical method applied in this study was found suitable, firstly, in profiling the samples based on correlation of multiple detection wavelengths and, secondly, in characterizing the total carbohydrate composition by grouping detected peaks by the compound type. Compounds were detected in their extracted form without timeconsuming pretreatment procedures. Differences in the raw material and process conditions were successfully identified by comparing characteristic peaks in the sample electropherograms of an example case.

Preparation and evaluation of alpha‐cellulose sulfate based new heterogeneous catalyst for production of biodiesel

“…There were two absorption peaks of crude alpha-cellulose at 282 and 344 nm, indicating that there were two non-equivalent C O groups as previously demonstrated. 26 However, after purification of the alpha-cellulose, the C O absorption peak appears as a single peak at 331 nm. The SR-UV spectrum of the alpha-cellulose sulfate product also shows a single absorption peak at 323 nm, indicating a hypsochromic shift due to the presence of sulfate moieties as an electron-withdrawing group.…”

Investigation of photochemical reaction products of glucose formed during direct UV detection in CE

“…By now, the phenomenon is ascribed to a fast base catalyzed photochemical reaction in the detection window of the separation capillary resulting in the formation of substances that exhibit UV absorption in the mentioned region . The applicability of this novel method of saccharide detection in CE has been demonstrated in various matrices . Other investigations dealt with the underlying photochemical reaction itself .…”

Wearable Label‐Free Optical Biodetectors: Progress and Perspectives

“…Glucose has a dominant absorbance peak at 260–270 nm in the UV wavelength range with an absorption ratio of 2.4. [ 97 ] The concentration of creatinine in the sweat corresponds to the absorption peak at 520 nm, [ 98 ] and important stress biomarker cortisol has a detectable absorption peak in the UV region of 247 nm. [ 99 ] Another important biomarker bilirubin which is used to diagnose jaundice in new born babies, acute chronic liver and kidney diseases, and cardiovascular diseases has a detectable absorption peak at 460 nm.…”