Profile of Aliarcobacter spp. from edible giblets: Genetic diversity, antibiotic resistance, biofilm formation

Güngör, Candan; Hızlısoy, Harun; Onmaz, Nurhan Ertaş; Gundog, Dursun Alp; Barel, Mukaddes; Disli, H. Burak; Dışhan, Adalet; Al, Serhat; Yıldırım, Yeliz; Gönülalan, Zafer

doi:10.1016/j.ijfoodmicro.2022.110047

Cited by 2 publications

(5 citation statements)

References 49 publications

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“…When the tetracycline resistance genes (tetW and tetO) were evaluated, it was determined that 7 (31.8%) A. butzleri isolates were positive for the tetW gene, while no isolates were positive for the tetO gene. According toGungor et al, (2023), Twenty-four (85.7%) of 28 isolates were positive for the tetW gene, but none of the isolates harbour the tetO gene, similar to our ndings. presence of tetO and tetW genes, which are known to be coding for tetracycline resistance, and simultaneous antibiotic resistance(Gungor et al 2023).…”

supporting

confidence: 88%

“…According toGungor et al, (2023), Twenty-four (85.7%) of 28 isolates were positive for the tetW gene, but none of the isolates harbour the tetO gene, similar to our ndings. presence of tetO and tetW genes, which are known to be coding for tetracycline resistance, and simultaneous antibiotic resistance(Gungor et al 2023). It might be explained by the presence of other genetic elements playing an important role in antibiotic resistance(Hedayatianfard et al 2014).When considered that a strain is multidrug resistant (MDR) if it is resistant to three or more classes of antibiotics; 81.8% of the strains isolated in this study presented an MDR phenotype, of which 84.2% were among A. butzleri and 100% were among A. cryaerophilus and A. skirrowii isolates.…”

supporting

confidence: 88%

“…Resistance to ampicillin has been reported previously for A. butzleri isolated from different sources (Ferreira et Gungor et al 2023). Due to the deterioration of treatment of serious infections, also to the possible transfer of resistance markers through the consumption of contaminated food with microorganisms resistant to different antibiotics, resistance to antibiotics can be assumed as a relevant public health problem (Rahimi 2014).…”

Section: Discussionsupporting

confidence: 56%

“…It was observed that almost half of the isolates (47.3%) were able to produce bio lm in three different levels. In many studies on the bio lm ability of Aliarcobacter spp., it has been reported that they have different levels of bio lm production capacity (Girbau et Gungor et al 2023). The ability to form bio lms can vary greatly between strains of the same species, depending on exogenous factors such as nutrient availability, environmental conditions, and surface properties (Girbau et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…isolated from slaughterhouses samples showed genetic diversity and heterogeneous populations despite their ancestry. Gungor et al (2023) grouped Aliarcobacter spp. isolates obteined from edible giblets into three main clusters (A-C) and one singleton.…”

Section: Discussionmentioning

confidence: 99%

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Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

Disli,

Hizlisoy,

Gungor

et al. 2024

Int Microbiol

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Aliarcobacter spp. have has been isolated from numerous food products at retail and from animal carcasses and feces at slaughter. The objectives of this study were: i) to isolate Aliarcobacter species (Aliarcobacter butzleri, Aliarcobacter skirrowii, Aliarcobacter cryaerophilus) from different slaughterhouses' samples, ii) to detect genetic diversity, antibiotic resistance, bio lm ability and putative virulence gene pro les of the isolates. The antibiotic susceptibility to antimicrobials was examined using the Kirby-Bauer disc diffusion method. A molecular investigation of antibiotic resistance and virulence factors was also conducted using polymerase chain reaction (PCR).A total of 22 (14.6%) Aliarcobacter spp. isolates were obtained, with varying levels of antibiotic resistance observed. The genes tetO, tetW, and gyrA were detected in 0%, 31.8%, and 27.2% of the isolates, respectively. All isolates were resistant to ampicillin, rifampin, and erythromycin, while tetracycline was found to be the most effective antibiotic, with 81.8% of the isolates showing susceptibility to it. In terms of virulence factors, all isolates (100%) harbored more than one of the nine putative virulence genes tested, with 18.1% of isolates carrying more than three.Regarding bio lm formation, 7 (31.8%) and 4 (18.1%) isolates were found to form strong and moderate bio lms, respectively, while one (4.5%) isolate was classi ed as a weak bio lm producer. ERIC-PCR band patterns suggested that the isolated Aliarcobacter spp. from slaughterhouses had different sources of contamination. These ndings highlight the potential risk posed by pathogenic and multidrug-resistant Aliarcobacter spp. in food, and the need for control measures throughout the food chain to prevent the spread of these strains. The results indicate that foods of animal origin and cattle slaughterhouses are signi cant sources of antimicrobial resistant Aliarcobacters.

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supporting

confidence: 88%

supporting

confidence: 88%

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

Disli,

Hizlisoy,

Gungor

et al. 2024

Int Microbiol

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Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

Disli

Hızlısoy

Güngör

et al. 2023

Preprint

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Aliarcobacter spp. have has been isolated from numerous food products at retail and from animal carcasses and feces at slaughter. The objectives of this study were: i) to isolate Aliarcobacter species (Aliarcobacter butzleri, Aliarcobacter skirrowii, Aliarcobacter cryaerophilus) from different slaughterhouses’ samples, ii) to detect genetic diversity, antibiotic resistance, biofilm ability and putative virulence gene profiles of the isolates. The antibiotic susceptibility to antimicrobials was examined using the Kirby-Bauer disc diffusion method. A molecular investigation of antibiotic resistance and virulence factors was also conducted using polymerase chain reaction (PCR). A total of 22 (14.6%) Aliarcobacter spp. isolates were obtained, with varying levels of antibiotic resistance observed. The genes tetO, tetW, and gyrA were detected in 0%, 31.8%, and 27.2% of the isolates, respectively. All isolates were resistant to ampicillin, rifampin, and erythromycin, while tetracycline was found to be the most effective antibiotic, with 81.8% of the isolates showing susceptibility to it. In terms of virulence factors, all isolates (100%) harbored more than one of the nine putative virulence genes tested, with 18.1% of isolates carrying more than three. Regarding biofilm formation, 7 (31.8%) and 4 (18.1%) isolates were found to form strong and moderate biofilms, respectively, while one (4.5%) isolate was classified as a weak biofilm producer. ERIC-PCR band patterns suggested that the isolated Aliarcobacter spp. from slaughterhouses had different sources of contamination. These findings highlight the potential risk posed by pathogenic and multidrug-resistant Aliarcobacter spp. in food, and the need for control measures throughout the food chain to prevent the spread of these strains. The results indicate that foods of animal origin and cattle slaughterhouses are significant sources of antimicrobial resistant Aliarcobacters.

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Profile of Aliarcobacter spp. from edible giblets: Genetic diversity, antibiotic resistance, biofilm formation

Cited by 2 publications

References 49 publications

Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

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