Productivity of a preparative high‐performance liquid chromatography isolation of anacardic acids from cashew nut shell liquid

Filho, Francisco Oiram; Zocolo, Guilherme Julião; Canuto, Kirley Marques; Silva, Ivanildo J. da; Brito, Edy Sousa de

doi:10.1002/sscp.201900014

Cited by 6 publications

(6 citation statements)

References 39 publications

(42 reference statements)

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“…It can be seen that the peak at about 22 min increased with each increase in the ratio of CNSL. This was estimated to be the peak of anacardic acid, one of the main components of CNSL [30]. The calibration curves were drawn with the area of the main peak represen thitsiol 16 in Figures 5 and 6 on the y-axis and the concentration of the blended lacq samples on the x-axis.…”

Section: Blind Test For Quantification Of a Mixed Lacquer Sapmentioning

confidence: 99%

Quantitative Detection of Thitsiol and Urushiol as Markers from the Gluta usitata Lacquer Tree Using HPLC

Lee,

Lee

et al. 2023

Molecules

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Lacquer sap has been traditionally used in coatings and artwork. Suitable types of lacquer are required to preserve and restore artifacts. Recently, unsuitable cashew nut shell liquid (CNSL) has often been mixed with lacquer sap, so it is necessary to identify the characteristics of lacquer sap by the production area. However, research is still focused on urushiol and laccol. In this study, Myanmarese lacquer sap collected from Gluta usitata, which contains thitsiol as the main component, was analyzed by HPLC to quantify thitsiol using the standards 3-(10-phenyldecyl) benzene-1,2-diol (thitsiol 16) and 3-(8Z,11Z-pentadecadienyl)-benzenediol (urushiol 15:2) as markers, and calibration curves were plotted. The coefficients of determination (R2) for thitsiol 16 and urushiol 15:2 were 0.9985 and 0.9983, respectively. In addition, a blind test was conducted to confirm that accurate quantitative analysis was possible even when Myanmarese lacquer was mixed with lacquer from another production area, which contained urushiol as the main component, and CNSL, which contained cardol, a completely different catechol. Quantitative analysis of thitsiol 16 and urushiol 15:2 in Myanmarese lacquer using HPLC can be used to evaluate the quality of lacquer sap and for more sophisticated activities such as restoration by classifying differences in lacquer sap by the production area.

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Section: Blind Test For Quantification Of a Mixed Lacquer Sapmentioning

confidence: 99%

Quantitative Detection of Thitsiol and Urushiol as Markers from the Gluta usitata Lacquer Tree Using HPLC

Lee,

Lee

et al. 2023

Molecules

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“…(purity ≥ 96%) were from Sigma-Aldrich (Saint Louis, MO, USA), and ultra-pure water (type 1) was obtained from a Milli-Q system (São Paulo, Brazil). The triene AnAc standard (15:3) used to obtain the analytical curve was obtained by the method described previously [11].…”

Section: Chemicals and Standardsmentioning

confidence: 99%

“…Structurally, AnAc are classified as phenolic lipids because they have a long carbon chain with structural variations. The number of carbon atoms present in this chain can vary between 2 and 17 [10,11], with the number of unsaturated C=C bonds ranging between 0 and 3 [11,12]. However, the structural differences between AnAc can give them different biological activities [13] and sensory properties [14].…”

Section: Introductionmentioning

confidence: 99%

Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector

Filho

Mitri

Zocolo

et al. 2023

Foods

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The cashew peduncle has a high nutritional value and contains a wide variety of phenolic compounds. Among these, anacardic acids (AnAc) are biologically active components; however, they influence the cashew juice flavor and, consequently, its acceptance. This study validates a high-performance liquid chromatography method for quantifying the AnAc present in cashew peduncles, using a C18 reverse-phase column and a diode-array detector. The calibration curve obtained showed satisfactory precision for intraday (CV = 0.20%) and interday (CV = 0.29%) quantification, linearity (y = 2333.5x + 2956.2; r2 = 0.9979), repeatability with respect to retention time (CV = 0.45%) and area (CV = 0.30%), and selectivity, and possessed detection and quantification limits of 0.18 and 0.85 µg·mL−1, respectively. Different cashew clones containing AnAc were extracted and analyzed using the proposed method. A recovery of >90% was achieved using two sequential extractions. The total AnAc content ranged from 128.35 to 217.00 mg·100 g−1 in peduncle samples obtained from five different cashew clones.

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“…Anacardic acids were obtained by preparative HPLC fractionation of cashew nut shell liquid (CNSL) as described by Oiram Filho, Zocolo, Canuto, Silva Junior, and de Brito, 2019. 9 The compounds present in the anacardic acid mixture were isolated on a reverse phase chromatographic column Waters SunFirePrep C18 OBD (100 x 19 mm x 5 μm). The mobile phase was used in an isocratic mode using methanol, water and acetic acid in the proportion (90:10:1), run time of 40 min, and a flow of 3 mL.min -1 , at 25 ºC.…”

Section: Fractionation Of Anacardic Acidsmentioning

confidence: 99%

Structure-Astringency Relationship of Anacardic Acids from Cashew Apple (Anacardium Occidentale L.)

Brito

Mendes²,

Garruti³

et al. 2020

Preprint

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Cashew apple presents a characteristic astringency. However, the compounds responsible for this characteristic were not described yet. A cashew apple extract was added to a BSA solution and the compounds before and after precipitation were analyzed by UPLC-QTOF/MSE. The extract astringency was measured on a 5-point scale (0: non astringent and 4: extremely astringent). Among the phenolics detected anacardic acids were identified and evaluated for their astringent effect. In the sensorial tests the cashew apple extract was considered very astringent (average of 2.5). A mixture of anacardic acids, had an average of 1.76 (astringent). The three isolated anacardic acids were evaluated. The in silicoexperiments were performed to analyze mainly the steric factor associated to the binding. The sensory results were confirmed by in silico analysis, indicating that a higher unsaturation degree of the aliphatic chain leads to an astringency increase.

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Productivity of a preparative high‐performance liquid chromatography isolation of anacardic acids from cashew nut shell liquid

Cited by 6 publications

References 39 publications

Quantitative Detection of Thitsiol and Urushiol as Markers from the Gluta usitata Lacquer Tree Using HPLC

Quantitative Detection of Thitsiol and Urushiol as Markers from the Gluta usitata Lacquer Tree Using HPLC

Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector

Structure-Astringency Relationship of Anacardic Acids from Cashew Apple (Anacardium Occidentale L.)

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