Production of α-Amylase and Amyloglucosidase by the Fungus <i>Cylindrocladium </i>sp<i>.</i> in Semi-solid State Fermentation

Abstract

A mylases are very important and have a wide range of applications. Semi-solid state fermentation is increasingly used for the production of enzymes, since it requires simple technology and costs are low. In this regard, it is worth emphasizing the importance of rice as a substrate, due to its readily availability and low cost. This study evaluated the ability of the fungus Cylindrocladium sp. to produce α-amylase and amy loglucosidase. The studies were carried out with the fungus strain Cylindrocladium sp. with a rice-based substrate. The material was kept in an oven at 28 °C for 96 hours and measured every 24 hours. Fo r the quantification of amy loglucosidase enzymes, the DNS reagent method was adopted. The α-amylase activity was determined by measuring the concentration of starch using iodine dosage. Analysis of the data obtained in this study showed that the fungus Cylindrocladium sp. produces α-amylase and amyloglucosidase enzymes; peak production for α-amy lase was at 72 hours with 245.02 U/g, and at 92 hours for amy loglucosidase with 73.58 U/g. Through the interpretation of these results it was possible to conclude that the fungus Cylindrocladium sp. produces relevant values of the enzy mes α-amylase and amy loglucosidase.

Structural Dependence of Sulfated Polysaccharide for Diabetes Management: Fucoidan From Undaria pinnatifida Inhibiting α-Glucosidase More Strongly Than α-Amylase and Amyloglucosidase

“…The inhibition activity of fucoidan against amyloglucosidase was determined by measuring the reducing power of released reducing sugar from soluble starch according to the method by Onofre et al (2012) with slight modification. The working enzyme solution was prepared freshly prior to use by diluting the stock amyloglucosidase solution (Megazyme, Ireland) to 6.5 U/ml in sodium phosphate buffer (SPB, 0.1 M, pH 6.9).…”

Cellulase Production by Endophytic Strains of &lt;i&gt;Trichoderma reesei&lt;/i&gt; from &lt;i&gt;Baccharis dracunculifolia&lt;/i&gt; D. C. (Asteraceae)

“…Reducing sugars were determined by reaction with 3,5-dinitrosalicylic acid (DNS) [18]. In a basic medium and at high temperature, this acid becomes 3-amino-5-nitrosalicylic acid, developing a red color and absorbing light at 540 nm.…”